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. 1994 Aug;176(15):4750–4753. doi: 10.1128/jb.176.15.4750-4753.1994

Tn5401 disruption of the spo0F gene, identified by direct chromosomal sequencing, results in CryIIIA overproduction in Bacillus thuringiensis.

T Malvar 1, J A Baum 1
PMCID: PMC196298  PMID: 8045906

Abstract

The Bacillus thuringiensis spo0F gene was identified by chromosomal DNA sequencing of sporulation mutants derived from a B. thuringiensis transposon insertion library. A spo0F defect in B. thuringiensis, which was suppressed by multicopy hknA or kinA, resulted in the overproduction of the CryIIIA insecticidal crystal protein.

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Selected References

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  1. Agaisse H., Lereclus D. Expression in Bacillus subtilis of the Bacillus thuringiensis cryIIIA toxin gene is not dependent on a sporulation-specific sigma factor and is increased in a spo0A mutant. J Bacteriol. 1994 Aug;176(15):4734–4741. doi: 10.1128/jb.176.15.4734-4741.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Antoniewski C., Savelli B., Stragier P. The spoIIJ gene, which regulates early developmental steps in Bacillus subtilis, belongs to a class of environmentally responsive genes. J Bacteriol. 1990 Jan;172(1):86–93. doi: 10.1128/jb.172.1.86-93.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baum J. A. Tn5401, a new class II transposable element from Bacillus thuringiensis. J Bacteriol. 1994 May;176(10):2835–2845. doi: 10.1128/jb.176.10.2835-2845.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Donovan W. P., Rupar M. J., Slaney A. C., Malvar T., Gawron-Burke M. C., Johnson T. B. Characterization of two genes encoding Bacillus thuringiensis insecticidal crystal proteins toxic to Coleoptera species. Appl Environ Microbiol. 1992 Dec;58(12):3921–3927. doi: 10.1128/aem.58.12.3921-3927.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kronstad J. W., Whiteley H. R. Three classes of homologous Bacillus thuringiensis crystal-protein genes. Gene. 1986;43(1-2):29–40. doi: 10.1016/0378-1119(86)90005-3. [DOI] [PubMed] [Google Scholar]
  6. Malvar T., Gawron-Burke C., Baum J. A. Overexpression of Bacillus thuringiensis HknA, a histidine protein kinase homology, bypasses early Spo mutations that result in CryIIIA overproduction. J Bacteriol. 1994 Aug;176(15):4742–4749. doi: 10.1128/jb.176.15.4742-4749.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Perego M., Cole S. P., Burbulys D., Trach K., Hoch J. A. Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis. J Bacteriol. 1989 Nov;171(11):6187–6196. doi: 10.1128/jb.171.11.6187-6196.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Sekar V., Thompson D. V., Maroney M. J., Bookland R. G., Adang M. J. Molecular cloning and characterization of the insecticidal crystal protein gene of Bacillus thuringiensis var. tenebrionis. Proc Natl Acad Sci U S A. 1987 Oct;84(20):7036–7040. doi: 10.1073/pnas.84.20.7036. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Smith I., Dubnau E., Predich M., Bai U., Rudner R. Early spo gene expression in Bacillus subtilis: the role of interrelated signal transduction systems. Biochimie. 1992 Jul-Aug;74(7-8):669–678. doi: 10.1016/0300-9084(92)90139-6. [DOI] [PubMed] [Google Scholar]
  10. Trach K. A., Chapman J. W., Piggot P. J., Hoch J. A. Deduced product of the stage 0 sporulation gene spo0F shares homology with the Spo0A, OmpR, and SfrA proteins. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7260–7264. doi: 10.1073/pnas.82.21.7260. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. de Souza M. T., Lecadet M. M., Lereclus D. Full expression of the cryIIIA toxin gene of Bacillus thuringiensis requires a distant upstream DNA sequence affecting transcription. J Bacteriol. 1993 May;175(10):2952–2960. doi: 10.1128/jb.175.10.2952-2960.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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