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. 1994 Aug;176(16):5116–5122. doi: 10.1128/jb.176.16.5116-5122.1994

Structural and functional analyses of mutant Fur proteins with impaired regulatory function.

A M Wertheimer 1, M E Tolmasky 1, L A Actis 1, J H Crosa 1
PMCID: PMC196352  PMID: 8051025

Abstract

Vibrio anguillarum Fur mutants, 775met9 and 775met11, were characterized. V. anguillarum 775met9 had a change of D to G at position 104 located in the carboxy terminus resulting in impaired Fur activity.Computer analysis predicts perturbation of an alpha-helix in the carboxy terminus which may interfere with Fur protein conformation. Strain 775met11 had a change in the start codon resulting in no protein synthesis. The mutants are unstable, and reversion to the wild type occurs frequently.

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Selected References

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