Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Aug;176(16):5167–5170. doi: 10.1128/jb.176.16.5167-5170.1994

The Abundance of atp Gene Transcript and of the Membrane F1F0-ATPase as a Function of the Growth pH of Alkaliphilic Bacillus firmus OF4

D Mack Ivey 1,†, Michael G Sturr 1, Terry A Krulwich 1, David B Hicks 1,*
PMCID: PMC196363  PMID: 7519597

Abstract

Molecular biological and biochemical studies of the F1F0-ATP synthase of alkaliphilic Bacillus firmus OF4 show that the enzyme used at pH 7.5 and pH 10.5 is a unique product of the atp operon, expressed at the same levels and yielding an enzyme with the same subunit properties and c-subunit/holoenzyme stoichiometry.

Full text

PDF

Images in this article

5168Image
on p.5168
5168Image
on p.5168
5168Image
on p.5168
5169Image
on p.5169

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beechey R. B., Linnett P. E., Fillingame R. H. Isolation of carbodiimide-binding proteins from mitochondria and Escherichia coli. Methods Enzymol. 1979;55:426–434. doi: 10.1016/0076-6879(79)55057-5. [DOI] [PubMed] [Google Scholar]
  2. Brusilow W. S., Scarpetta M. A., Hawthorne C. A., Clark W. P. Organization and sequence of the genes coding for the proton-translocating ATPase of Bacillus megaterium. J Biol Chem. 1989 Jan 25;264(3):1528–1533. [PubMed] [Google Scholar]
  3. Cross R. L., Taiz L. Gene duplication as a means for altering H+/ATP ratios during the evolution of FoF1 ATPases and synthases. FEBS Lett. 1990 Jan 1;259(2):227–229. doi: 10.1016/0014-5793(90)80014-a. [DOI] [PubMed] [Google Scholar]
  4. Fillingame R. H. Purification of the carbodiimide-reactive protein component of the ATP energy-transducing system of Escherichia coli. J Biol Chem. 1976 Nov 10;251(21):6630–6637. [PubMed] [Google Scholar]
  5. Futai M., Noumi T., Maeda M. Molecular genetics of F1-ATPase from Escherichia coli. J Bioenerg Biomembr. 1988 Feb;20(1):41–58. doi: 10.1007/BF00762137. [DOI] [PubMed] [Google Scholar]
  6. Guffanti A. A., Finkelthal O., Hicks D. B., Falk L., Sidhu A., Garro A., Krulwich T. A. Isolation and characterization of new facultatively alkalophilic strains of Bacillus species. J Bacteriol. 1986 Sep;167(3):766–773. doi: 10.1128/jb.167.3.766-773.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hicks D. B., Krulwich T. A. Purification and reconstitution of the F1F0-ATP synthase from alkaliphilic Bacillus firmus OF4. Evidence that the enzyme translocates H+ but not Na+. J Biol Chem. 1990 Nov 25;265(33):20547–20554. [PubMed] [Google Scholar]
  8. Hicks D. B., Plass R. J., Quirk P. G. Evidence for multiple terminal oxidases, including cytochrome d, in facultatively alkaliphilic Bacillus firmus OF4. J Bacteriol. 1991 Aug;173(16):5010–5016. doi: 10.1128/jb.173.16.5010-5016.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ivey D. M., Krulwich T. A. Organization and nucleotide sequence of the atp genes encoding the ATP synthase from alkaliphilic Bacillus firmus OF4. Mol Gen Genet. 1991 Oct;229(2):292–300. doi: 10.1007/BF00272169. [DOI] [PubMed] [Google Scholar]
  10. Kobayashi H., Suzuki T., Unemoto T. Streptococcal cytoplasmic pH is regulated by changes in amount and activity of a proton-translocating ATPase. J Biol Chem. 1986 Jan 15;261(2):627–630. [PubMed] [Google Scholar]
  11. Krulwich T. A., Guffanti A. A. Proton-coupled bioenergetic processes in extremely alkaliphilic bacteria. J Bioenerg Biomembr. 1992 Dec;24(6):587–599. doi: 10.1007/BF00762351. [DOI] [PubMed] [Google Scholar]
  12. Ladin B. F., Accuroso E. M., Mielenz J. R., Wilson C. R. A rapid procedure for isolating RNA from small-scale Bacillus cultures. Biotechniques. 1992 May;12(5):672–676. [PubMed] [Google Scholar]
  13. Quirk P. G., Hicks D. B., Krulwich T. A. Cloning of the cta operon from alkaliphilic Bacillus firmus OF4 and characterization of the pH-regulated cytochrome caa3 oxidase it encodes. J Biol Chem. 1993 Jan 5;268(1):678–685. [PubMed] [Google Scholar]
  14. Schägger H., von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987 Nov 1;166(2):368–379. doi: 10.1016/0003-2697(87)90587-2. [DOI] [PubMed] [Google Scholar]
  15. Simpson R. J., Nice E. C. In situ cyanogen bromide cleavage of N-terminally blocked proteins in a gas-phase sequencer. Biochem Int. 1984 Jun;8(6):787–791. [PubMed] [Google Scholar]
  16. Sturr M. G., Guffanti A. A., Krulwich T. A. Growth and bioenergetics of alkaliphilic Bacillus firmus OF4 in continuous culture at high pH. J Bacteriol. 1994 Jun;176(11):3111–3116. doi: 10.1128/jb.176.11.3111-3116.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES