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. 1994 Aug;176(16):5177–5180. doi: 10.1128/jb.176.16.5177-5180.1994

Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autolysin operon of Bacillus subtilis.

T Tokunaga 1, M H Rashid 1, A Kuroda 1, J Sekiguchi 1
PMCID: PMC196365  PMID: 7914190

Abstract

Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene constructed in the B. subtilis chromosome. The phosphorylated form of the DegU protein seems to be a regulator for expression of the sigD gene.

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Selected References

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