Abstract
Flagellated envelopes of Halobacterium salinarium cells were prepared by lysis with taurodeoxycholate. After solubilization of the envelopes with Triton X-100 at high ionic strength, flagella and round patches from which numerous flagella emerged were isolated by gel filtration chromatography. We conclude that the flagellar bundle of H. salinarium is inserted into a differentiated polar cap structure.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Alam M., Oesterhelt D. Morphology, function and isolation of halobacterial flagella. J Mol Biol. 1984 Jul 15;176(4):459–475. doi: 10.1016/0022-2836(84)90172-4. [DOI] [PubMed] [Google Scholar]
- Alam M., Oesterhelt D. Purification, reconstitution and polymorphic transition of halobacterial flagella. J Mol Biol. 1987 Apr 5;194(3):495–499. doi: 10.1016/0022-2836(87)90677-2. [DOI] [PubMed] [Google Scholar]
- Coulton J. W., Murray R. G. Membrane-associated components of the bacterial flagellar apparatus. Biochim Biophys Acta. 1977 Mar 1;465(2):290–310. doi: 10.1016/0005-2736(77)90080-3. [DOI] [PubMed] [Google Scholar]
- Ferris F. G., Beveridge T. J., Marceau-Day M. L., Larson A. D. Structure and cell envelope associations of flagellar basal complexes of Vibrio cholerae and Campylobacter fetus. Can J Microbiol. 1984 Mar;30(3):322–333. doi: 10.1139/m84-048. [DOI] [PubMed] [Google Scholar]
- HOUWINK A. L. Flagella, gas vacuoles and cell-wall structure in Halobacterium halobium; an electron microscope study. J Gen Microbiol. 1956 Aug;15(1):146–150. doi: 10.1099/00221287-15-1-146. [DOI] [PubMed] [Google Scholar]
- Kalmokoff M. L., Jarrell K. F., Koval S. F. Isolation of flagella from the archaebacterium Methanococcus voltae by phase separation with Triton X-114. J Bacteriol. 1988 Apr;170(4):1752–1758. doi: 10.1128/jb.170.4.1752-1758.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kamekura M., Oesterhelt D., Wallace R., Anderson P., Kushner D. J. Lysis of halobacteria in bacto-peptone by bile acids. Appl Environ Microbiol. 1988 Apr;54(4):990–995. doi: 10.1128/aem.54.4.990-995.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kupper J., Wildhaber I., Gao Z., Baeuerlein E. Basal-body-associated disks are additional structural elements of the flagellar apparatus isolated from Wolinella succinogenes. J Bacteriol. 1989 May;171(5):2803–2810. doi: 10.1128/jb.171.5.2803-2810.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marwan W., Alam M., Oesterhelt D. Rotation and switching of the flagellar motor assembly in Halobacterium halobium. J Bacteriol. 1991 Mar;173(6):1971–1977. doi: 10.1128/jb.173.6.1971-1977.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Oesterhelt D., Krippahl G. Phototrophic growth of halobacteria and its use for isolation of photosynthetically-deficient mutants. Ann Microbiol (Paris) 1983 Jul-Aug;134B(1):137–150. doi: 10.1016/s0769-2609(83)80101-x. [DOI] [PubMed] [Google Scholar]
- Schuster S. C., Baeuerlein E. Location of the basal disk and a ringlike cytoplasmic structure, two additional structures of the flagellar apparatus of Wolinella succinogenes. J Bacteriol. 1992 Jan;174(1):263–268. doi: 10.1128/jb.174.1.263-268.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spudich E. N., Spudich J. L. Control of transmembrane ion fluxes to select halorhodopsin-deficient and other energy-transduction mutants of Halobacterium halobium. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4308–4312. doi: 10.1073/pnas.79.14.4308. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sumper M. Halobacterial glycoprotein biosynthesis. Biochim Biophys Acta. 1987 Apr 27;906(1):69–79. doi: 10.1016/0304-4157(87)90005-0. [DOI] [PubMed] [Google Scholar]
- Wieland F., Paul G., Sumper M. Halobacterial flagellins are sulfated glycoproteins. J Biol Chem. 1985 Dec 5;260(28):15180–15185. [PubMed] [Google Scholar]