Abstract
The zymogen of the protease (GPR) that initiates protein degradation during spore germination in Bacillus species is not activated in vitro under normal physiological conditions. However, there is rapid, acid-pH-dependent, zero-order, proteolytic activation of the purified zymogen in high concentrations of dimethyl sulfoxide. These findings provide further evidence that GPR activates itself during sporulation.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Henderson T. R., Henderson R. F., York J. L. Effects of dimethyl sulfoxide on subunit proteins. Ann N Y Acad Sci. 1975 Jan 27;243:38–53. doi: 10.1111/j.1749-6632.1975.tb25342.x. [DOI] [PubMed] [Google Scholar]
- Illades-Aguiar B., Setlow P. Studies of the processing of the protease which initiates degradation of small, acid-soluble proteins during germination of spores of Bacillus species. J Bacteriol. 1994 May;176(10):2788–2795. doi: 10.1128/jb.176.10.2788-2795.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jackson M., Mantsch H. H. Beware of proteins in DMSO. Biochim Biophys Acta. 1991 Jun 24;1078(2):231–235. doi: 10.1016/0167-4838(91)90563-f. [DOI] [PubMed] [Google Scholar]
- Loshon C. A., Setlow P. Bacillus megaterium spore protease: purification, radioimmunoassay, and analysis of antigen level and localization during growth, sporulation, and spore germination. J Bacteriol. 1982 Apr;150(1):303–311. doi: 10.1128/jb.150.1.303-311.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Magill N. G., Cowan A. E., Koppel D. E., Setlow P. The internal pH of the forespore compartment of Bacillus megaterium decreases by about 1 pH unit during sporulation. J Bacteriol. 1994 Apr;176(8):2252–2258. doi: 10.1128/jb.176.8.2252-2258.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maurel P. Relevance of dielectric constant and solvent hydrophobicity to the organic solvent effect in enzymology. J Biol Chem. 1978 Mar 10;253(5):1677–1683. [PubMed] [Google Scholar]
- Robinson C. R., Sligar S. G. Molecular recognition mediated by bound water. A mechanism for star activity of the restriction endonuclease EcoRI. J Mol Biol. 1993 Nov 20;234(2):302–306. doi: 10.1006/jmbi.1993.1586. [DOI] [PubMed] [Google Scholar]
- Sanchez-Salas J. L., Santiago-Lara M. L., Setlow B., Sussman M. D., Setlow P. Properties of Bacillus megaterium and Bacillus subtilis mutants which lack the protease that degrades small, acid-soluble proteins during spore germination. J Bacteriol. 1992 Feb;174(3):807–814. doi: 10.1128/jb.174.3.807-814.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanchez-Salas J. L., Setlow P. Proteolytic processing of the protease which initiates degradation of small, acid-soluble proteins during germination of Bacillus subtilis spores. J Bacteriol. 1993 May;175(9):2568–2577. doi: 10.1128/jb.175.9.2568-2577.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Setlow P. Purification and properties of a specific proteolytic enzyme present in spores of Bacillus magaterium. J Biol Chem. 1976 Dec 25;251(24):7853–7862. [PubMed] [Google Scholar]
- Setlow P. Small, acid-soluble spore proteins of Bacillus species: structure, synthesis, genetics, function, and degradation. Annu Rev Microbiol. 1988;42:319–338. doi: 10.1146/annurev.mi.42.100188.001535. [DOI] [PubMed] [Google Scholar]
- Sussman M. D., Setlow P. Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination. J Bacteriol. 1991 Jan;173(1):291–300. doi: 10.1128/jb.173.1.291-300.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]