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. 1994 Sep;176(18):5831–5834. doi: 10.1128/jb.176.18.5831-5834.1994

Overproduction, isolation, and DNA-binding characteristics of Xre, the repressor protein from the Bacillus subtilis defective prophage PBSX.

G E McDonnell 1, D J McConnell 1
PMCID: PMC196788  PMID: 8083175

Abstract

PBSX is a phage-like bacteriocin (phibacin) of Bacillus subtilis 168. Lysogeny is maintained by the PBSX-encoded repressor, Xre. The Xre protein was overproduced in Escherichia coli and isolated by affinity chromatography. Gel retardation and DNase I footprinting studies indicated that Xre binds to four sites close to its own gene. These sites overlap putative promoters for xre and a divergent transcriptional unit, containing the middle genes.

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Selected References

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