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. 1993 Feb;67(2):262–267. doi: 10.1038/bjc.1993.50

Syrian hamster embryo cell lines useful for detecting transforming genes in mouse tumours: detection of transforming genes in X-ray-related mouse tumours.

H Sasai 1, T Higashi 1, S Nakamori 1, J Miyoshi 1, F Suzuki 1, T Nomura 1, T Kakunaga 1
PMCID: PMC1968199  PMID: 8431357

Abstract

The Syrian hamster embryo cell lines, SHOK and MC-1, were used as recipient cells for DNA transfection assay to detect transforming genes in experimental mouse tumours. A mouse repeat sequence was utilised to check whether each transformed focus included mouse genomic DNA in the Hamster background. We investigated five mouse tumours that are related to X-ray radiation, and detected activated c-K-ras, c-mos, and c-cot oncogenes which induced foci of hamster cells. These results show that SHOK and MC-1 cells have unique properties for detecting transforming genes in experimental mouse tumours.

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