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. 1993 Jan;67(1):76–80. doi: 10.1038/bjc.1993.12

Sulfolipids and glycolipid sulfotransferase activities in human renal cell carcinoma cells.

T Kobayashi 1, K Honke 1, K Kamio 1, N Sakakibara 1, S Gasa 1, N Miyao 1, T Tsukamoto 1, I Ishizuka 1, T Miyazaki 1, A Makita 1
PMCID: PMC1968242  PMID: 8094007

Abstract

A cell line (SMKT-R3) established from human renal cell carcinoma was characterized for the presence of sulfolipids and glycolipid sulfotransferases. Sulfolipids were found to constitute a large part of the acidic glycolipid fraction in SMKT-R3 cells. These findings were confirmed by metabolic labelling with 35S-sulfate. These sulfolipids were expressed at the surface of SMKT-R3 cells as ascertained by cytofluorometry using a monoclonal antibody directed to sulfolipids. Furthermore, markedly high activity levels of glycolipid sulfotransferases were observed in SMKT-R3 cells compared with other cell lines. These results suggest that the increased synthesis of sulfolipids in renal cell carcinoma tissue (Sakakibara et al., 1989. Cancer Res., 49, 335-339) is due to the elevation of the sulfotransferase activities of renal carcinoma cells themselves.

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Selected References

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