Abstract
We have recently succeeded in producing transgenic mice carrying a hybrid gene consisting of mouse metallothionein promoter-enhancer and the ret oncogene (MT/ret). (Iwamoto et al., 1991b). A retroperitoneal tumour developed in one of 17 MT/ret transgenic founder mice. Histological analysis revealed that the tumour consisted of undifferentiated neuroblasts and differentiated ganglion cells, the latter of which were strongly positive for neuron specific enolase. Expression of the ret transgene was observed at high levels in RNA from the tumour, but not in those of other normal tissues. In addition, a 100kDa ret protein was detected in the cell lysate of the tumour. Taken together with our previous data, these results suggest a possible role for the ret oncogene in the proliferation of neural crest cells.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
- Hamaguchi M., Grandori C., Hanafusa H. Phosphorylation of cellular proteins in Rous sarcoma virus-infected cells: analysis by use of anti-phosphotyrosine antibodies. Mol Cell Biol. 1988 Aug;8(8):3035–3042. doi: 10.1128/mcb.8.8.3035. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Heisterkamp N., Jenster G., ten Hoeve J., Zovich D., Pattengale P. K., Groffen J. Acute leukaemia in bcr/abl transgenic mice. Nature. 1990 Mar 15;344(6263):251–253. doi: 10.1038/344251a0. [DOI] [PubMed] [Google Scholar]
- Ikeda I., Ishizaka Y., Tahira T., Suzuki T., Onda M., Sugimura T., Nagao M. Specific expression of the ret proto-oncogene in human neuroblastoma cell lines. Oncogene. 1990 Sep;5(9):1291–1296. [PubMed] [Google Scholar]
- Iwamoto T., Pu M., Ito M., Takahashi M., Isobe K., Nagase F., Kawashima K., Ichihara M., Nakashima I. Preferential development of pre-B lymphomas with drastically down-regulated N-myc in the E mu-ret transgenic mice. Eur J Immunol. 1991 Aug;21(8):1809–1814. doi: 10.1002/eji.1830210805. [DOI] [PubMed] [Google Scholar]
- Iwamoto T., Takahashi M., Ito M., Hamaguchi M., Isobe K., Misawa N., Asai J., Yoshida T., Nakashima I. Oncogenicity of the ret transforming gene in MMTV/ret transgenic mice. Oncogene. 1990 Apr;5(4):535–542. [PubMed] [Google Scholar]
- Iwamoto T., Takahashi M., Ito M., Hamatani K., Ohbayashi M., Wajjwalku W., Isobe K., Nakashima I. Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene. EMBO J. 1991 Nov;10(11):3167–3175. doi: 10.1002/j.1460-2075.1991.tb04878.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Messing A., Chen H. Y., Palmiter R. D., Brinster R. L. Peripheral neuropathies, hepatocellular carcinomas and islet cell adenomas in transgenic mice. Nature. 1985 Aug 1;316(6027):461–463. doi: 10.1038/316461a0. [DOI] [PubMed] [Google Scholar]
- Nagao M., Ishizaka Y., Nakagawara A., Kohno K., Kuwano M., Tahira T., Itoh F., Ikeda I., Sugimura T. Expression of ret proto-oncogene in human neuroblastomas. Jpn J Cancer Res. 1990 Apr;81(4):309–312. doi: 10.1111/j.1349-7006.1990.tb02566.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rüther U., Garber C., Komitowski D., Müller R., Wagner E. F. Deregulated c-fos expression interferes with normal bone development in transgenic mice. 1987 Jan 29-Feb 4Nature. 325(6103):412–416. doi: 10.1038/325412a0. [DOI] [PubMed] [Google Scholar]
- Santoro M., Rosati R., Grieco M., Berlingieri M. T., D'Amato G. L., de Franciscis V., Fusco A. The ret proto-oncogene is consistently expressed in human pheochromocytomas and thyroid medullary carcinomas. Oncogene. 1990 Oct;5(10):1595–1598. [PubMed] [Google Scholar]
- Tahira T., Ishizaka Y., Itoh F., Sugimura T., Nagao M. Characterization of ret proto-oncogene mRNAs encoding two isoforms of the protein product in a human neuroblastoma cell line. Oncogene. 1990 Jan;5(1):97–102. [PubMed] [Google Scholar]
- Takahashi M., Buma Y., Hiai H. Isolation of ret proto-oncogene cDNA with an amino-terminal signal sequence. Oncogene. 1989 Jun;4(6):805–806. [PubMed] [Google Scholar]
- Takahashi M., Buma Y., Iwamoto T., Inaguma Y., Ikeda H., Hiai H. Cloning and expression of the ret proto-oncogene encoding a tyrosine kinase with two potential transmembrane domains. Oncogene. 1988 Nov;3(5):571–578. [PubMed] [Google Scholar]
- Takahashi M., Buma Y., Taniguchi M. Identification of the ret proto-oncogene products in neuroblastoma and leukemia cells. Oncogene. 1991 Feb;6(2):297–301. [PubMed] [Google Scholar]
- Takahashi M., Cooper G. M. ret transforming gene encodes a fusion protein homologous to tyrosine kinases. Mol Cell Biol. 1987 Apr;7(4):1378–1385. doi: 10.1128/mcb.7.4.1378. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takahashi M., Inaguma Y., Hiai H., Hirose F. Developmentally regulated expression of a human "finger"-containing gene encoded by the 5' half of the ret transforming gene. Mol Cell Biol. 1988 Apr;8(4):1853–1856. doi: 10.1128/mcb.8.4.1853. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takahashi M., Ritz J., Cooper G. M. Activation of a novel human transforming gene, ret, by DNA rearrangement. Cell. 1985 Sep;42(2):581–588. doi: 10.1016/0092-8674(85)90115-1. [DOI] [PubMed] [Google Scholar]
- Taniguchi M., Iwamoto T., Nakashima I., Nakayama A., Ohbayashi M., Matsuyama M., Takahashi M. Establishment and characterization of a malignant melanocytic tumor cell line expressing the ret oncogene. Oncogene. 1992 Aug;7(8):1491–1496. [PubMed] [Google Scholar]
- Wajjwalku W., Takahashi M., Miyaishi O., Lu J., Sakata K., Yokoi T., Saga S., Imai M., Matsuyama M., Hoshino M. Tissue distribution of mouse mammary tumor virus (MMTV) antigens and new endogenous MMTV loci in Japanese laboratory mouse strains. Jpn J Cancer Res. 1991 Dec;82(12):1413–1420. doi: 10.1111/j.1349-7006.1991.tb01814.x. [DOI] [PMC free article] [PubMed] [Google Scholar]