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. 1993 Jul;68(1):172–175. doi: 10.1038/bjc.1993.308

The identification of high and low risk groups for colorectal cancer using rectal mucosal crypt cell production rate (CCPR).

P S Rooney 1, P A Clarke 1, K A Gifford 1, J D Hardcastle 1, N C Armitage 1
PMCID: PMC1968319  PMID: 8318409

Abstract

Rectal mucosal proliferation was measured in 116 individuals using the metaphase arrest technique crypt cell production rate (CCPR). CCPR was found to be significantly elevated in individuals with adenomas (n = 42, CCPR = 13 cc c-1h-1, range 7-25 Cl 10-15) compared with normals (n = 21, CCPR = 10 cc c-1h-1 range 5-24 Cl 7-11, Mann-Whitney P = 0.001 z = 3.2). Mucosal proliferation was increased among individuals who were undergoing adenoma follow up but in whom no further adenomas were found (n = 37 CCPR = 12 range 5-26 cc c-1h-1 Cl 10-14) compared to controls (Mann-Whitney P = 0.01 z = 2.4) Proliferation in vegetarians i.e. low risk (n = 16) was similar to controls. Measurement of proliferative indices in rectal mucosa by the stathmokinetic technique CCPR can discriminate between high and low risk groups for colorectal cancer.

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Selected References

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  1. Allan A., Bristol J. B., Williamson R. C. Crypt cell production rate in ulcerative proctocolitis: differential increments in remission and relapse. Gut. 1985 Oct;26(10):999–1003. doi: 10.1136/gut.26.10.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appleton G. V., Wheeler E. E., Challacombe D. N., Williamson R. C. Validation of organ culture in colonic adaptation to surgical manipulation. Gut. 1991 Sep;32(9):1027–1030. doi: 10.1136/gut.32.9.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Atkin W. S., Morson B. C., Cuzick J. Long-term risk of colorectal cancer after excision of rectosigmoid adenomas. N Engl J Med. 1992 Mar 5;326(10):658–662. doi: 10.1056/NEJM199203053261002. [DOI] [PubMed] [Google Scholar]
  4. Barsoum G. H., Hendrickse C., Winslet M. C., Youngs D., Donovan I. A., Neoptolemos J. P., Keighley M. R. Reduction of mucosal crypt cell proliferation in patients with colorectal adenomatous polyps by dietary calcium supplementation. Br J Surg. 1992 Jun;79(6):581–583. doi: 10.1002/bjs.1800790639. [DOI] [PubMed] [Google Scholar]
  5. Bleiberg H., Mainguet P., Galand P. Cell renewal in familial polyposis: comparison between polyps and adjacent healthy mucosa. Gastroenterology. 1972 Aug;63(2):240–245. [PubMed] [Google Scholar]
  6. Deschner E. E., Lipkin M. Proliferative patterns in colonic mucosa in familial polyposis. Cancer. 1975 Feb;35(2):413–418. doi: 10.1002/1097-0142(197502)35:2<413::aid-cncr2820350217>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  7. Deschner E. E., Maskens A. P. Significance of the labeling index and labeling distribution as kinetic parameters in colorectal mucosa of cancer patients and DMH treated animals. Cancer. 1982 Sep 15;50(6):1136–1141. doi: 10.1002/1097-0142(19820915)50:6<1136::aid-cncr2820500617>3.0.co;2-a. [DOI] [PubMed] [Google Scholar]
  8. Fearon E. R., Vogelstein B. A genetic model for colorectal tumorigenesis. Cell. 1990 Jun 1;61(5):759–767. doi: 10.1016/0092-8674(90)90186-i. [DOI] [PubMed] [Google Scholar]
  9. Finney K. J., Ince P., Appleton D. R., Sunter J. P., Watson A. J. A comparison of crypt-cell proliferation in rat colonic mucosa in vivo and in vitro. J Anat. 1986 Dec;149:177–188. [PMC free article] [PubMed] [Google Scholar]
  10. Fireman Z., Rozen P., Fine N., Chetrit A. Reproducibility studies and effects of bowel preparations on measurements of rectal epithelial proliferation. Cancer Lett. 1989 Apr;45(1):59–64. doi: 10.1016/0304-3835(89)90036-0. [DOI] [PubMed] [Google Scholar]
  11. Hixson L. J., Fennerty M. B., Sampliner R. E., Garewal H. S. Prospective blinded trial of the colonoscopic miss-rate of large colorectal polyps. Gastrointest Endosc. 1991 Mar-Apr;37(2):125–127. doi: 10.1016/s0016-5107(91)70668-8. [DOI] [PubMed] [Google Scholar]
  12. Lipkin M., Blattner W. A., Gardner E. J., Burt R. W., Lynch H., Deschner E., Winawer S., Fraumeni J. F., Jr Classification and risk assessment of individuals with familial polyposis, Gardner's syndrome, and familial non-polyposis colon cancer from [3H]thymidine labeling patterns in colonic epithelial cells. Cancer Res. 1984 Sep;44(9):4201–4207. [PubMed] [Google Scholar]
  13. Lipkin M., Blattner W. E., Fraumeni J. F., Jr, Lynch H. T., Deschner E., Winawer S. Tritiated thymidine (phi p, phi h) labeling distribution as a marker for hereditary predisposition to colon cancer. Cancer Res. 1983 Apr;43(4):1899–1904. [PubMed] [Google Scholar]
  14. Lipkin M., Newmark H. Effect of added dietary calcium on colonic epithelial-cell proliferation in subjects at high risk for familial colonic cancer. N Engl J Med. 1985 Nov 28;313(22):1381–1384. doi: 10.1056/NEJM198511283132203. [DOI] [PubMed] [Google Scholar]
  15. Lipkin M., Uehara K., Winawer S., Sanchez A., Bauer C., Phillips R., Lynch H. T., Blattner W. A., Fraumeni J. F., Jr Seventh-Day Adventist vegetarians have a quiescent proliferative activity in colonic mucosa. Cancer Lett. 1985 Mar;26(2):139–144. doi: 10.1016/0304-3835(85)90019-9. [DOI] [PubMed] [Google Scholar]
  16. Phillips R. L., Garfinkel L., Kuzma J. W., Beeson W. L., Lotz T., Brin B. Mortality among California Seventh-Day Adventists for selected cancer sites. J Natl Cancer Inst. 1980 Nov;65(5):1097–1107. [PubMed] [Google Scholar]
  17. Phillips R. L. Role of life-style and dietary habits in risk of cancer among seventh-day adventists. Cancer Res. 1975 Nov;35(11 Pt 2):3513–3522. [PubMed] [Google Scholar]
  18. Pockros P. J., Foroozan P. Golytely lavage versus a standard colonoscopy preparation. Effect on normal colonic mucosal histology. Gastroenterology. 1985 Feb;88(2):545–548. doi: 10.1016/0016-5085(85)90519-0. [DOI] [PubMed] [Google Scholar]
  19. Ponz de Leon M., Roncucci L., Di Donato P., Tassi L., Smerieri O., Amorico M. G., Malagoli G., De Maria D., Antonioli A., Chahin N. J. Pattern of epithelial cell proliferation in colorectal mucosa of normal subjects and of patients with adenomatous polyps or cancer of the large bowel. Cancer Res. 1988 Jul 15;48(14):4121–4126. [PubMed] [Google Scholar]
  20. Potten C. S., Kellett M., Roberts S. A., Rew D. A., Wilson G. D. Measurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine. Gut. 1992 Jan;33(1):71–78. doi: 10.1136/gut.33.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Provenzale D., Garrett J. W., Condon S. E., Sandler R. S. Risk for colon adenomas in patients with rectosigmoid hyperplastic polyps. Ann Intern Med. 1990 Nov 15;113(10):760–763. doi: 10.7326/0003-4819-113-10-760. [DOI] [PubMed] [Google Scholar]
  22. Risio M., Lipkin M., Candelaresi G., Bertone A., Coverlizza S., Rossini F. P. Correlations between rectal mucosa cell proliferation and the clinical and pathological features of nonfamilial neoplasia of the large intestine. Cancer Res. 1991 Apr 1;51(7):1917–1921. [PubMed] [Google Scholar]
  23. Roncucci L., Ponz de Leon M., Scalmati A., Malagoli G., Pratissoli S., Perini M., Chahin N. J. The influence of age on colonic epithelial cell proliferation. Cancer. 1988 Dec 1;62(11):2373–2377. doi: 10.1002/1097-0142(19881201)62:11<2373::aid-cncr2820621120>3.0.co;2-y. [DOI] [PubMed] [Google Scholar]
  24. Rozen P., Fireman Z., Fine N., Wax Y., Ron E. Oral calcium suppresses increased rectal epithelial proliferation of persons at risk of colorectal cancer. Gut. 1989 May;30(5):650–655. doi: 10.1136/gut.30.5.650. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Terpstra O. T., van Blankenstein M., Dees J., Eilers G. A. Abnormal pattern of cell proliferation in the entire colonic mucosa of patients with colon adenoma or cancer. Gastroenterology. 1987 Mar;92(3):704–708. doi: 10.1016/0016-5085(87)90021-7. [DOI] [PubMed] [Google Scholar]
  26. Wilson R. G., Smith A. N., Bird C. C. Immunohistochemical detection of abnormal cell proliferation in colonic mucosa of subjects with polyps. J Clin Pathol. 1990 Sep;43(9):744–747. doi: 10.1136/jcp.43.9.744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Wright N. A., Appleton D. R. The metaphase arrest technique. A critical review. Cell Tissue Kinet. 1980 Nov;13(6):643–663. doi: 10.1111/j.1365-2184.1980.tb00503.x. [DOI] [PubMed] [Google Scholar]

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