Abstract
Evidence is reported here that alkalinization of Clostridium acetobutylicum cytoplasm involves hydrogenase activity. A decrease of in vivo hydrogenase activity is accompanied by intracellular accumulation of protons leading to a negative (interior acidic) pH gradient. However, the organism is able to maintain a constant proton motive force by interconverting chemical and electrical potentials.
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Selected References
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- Adams M. W., Mortenson L. E., Chen J. S. Hydrogenase. Biochim Biophys Acta. 1980 Dec;594(2-3):105–176. doi: 10.1016/0304-4173(80)90007-5. [DOI] [PubMed] [Google Scholar]
- Bakker E. P., Mangerich W. E. Interconversion of components of the bacterial proton motive force by electrogenic potassium transport. J Bacteriol. 1981 Sep;147(3):820–826. doi: 10.1128/jb.147.3.820-826.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Deppenmeier U., Blaut M., Mahlmann A., Gottschalk G. Reduced coenzyme F420: heterodisulfide oxidoreductase, a proton- translocating redox system in methanogenic bacteria. Proc Natl Acad Sci U S A. 1990 Dec 1;87(23):9449–9453. doi: 10.1073/pnas.87.23.9449. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hugenholtz J., Ljungdahl L. G. Electron transport and electrochemical proton gradient in membrane vesicles of Clostridium thermoautotrophicum. J Bacteriol. 1989 May;171(5):2873–2875. doi: 10.1128/jb.171.5.2873-2875.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kashket E. R. Stoichiometry of the H+-ATPase of Escherichia coli cells during anaerobic growth. FEBS Lett. 1983 Apr 18;154(2):343–346. doi: 10.1016/0014-5793(83)80179-3. [DOI] [PubMed] [Google Scholar]
- Kashket E. R. The proton motive force in bacteria: a critical assessment of methods. Annu Rev Microbiol. 1985;39:219–242. doi: 10.1146/annurev.mi.39.100185.001251. [DOI] [PubMed] [Google Scholar]
- Loubiere P., Salou P., Leroy M. J., Lindley N. D., Pareilleux A. Electrogenic malate uptake and improved growth energetics of the malolactic bacterium Leuconostoc oenos grown on glucose-malate mixtures. J Bacteriol. 1992 Aug;174(16):5302–5308. doi: 10.1128/jb.174.16.5302-5308.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maloney P. C. Relationship between phosphorylation potential and electrochemical H+ gradient during glycolysis in Streptococcus lactis. J Bacteriol. 1983 Mar;153(3):1461–1470. doi: 10.1128/jb.153.3.1461-1470.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michels M., Bakker E. P. Low-affinity potassium uptake system in Bacillus acidocaldarius. J Bacteriol. 1987 Sep;169(9):4335–4341. doi: 10.1128/jb.169.9.4335-4341.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mitchell P. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc. 1966 Aug;41(3):445–502. doi: 10.1111/j.1469-185x.1966.tb01501.x. [DOI] [PubMed] [Google Scholar]
- Odom J. M., Peck H. D., Jr Hydrogenase, electron-transfer proteins, and energy coupling in the sulfate-reducing bacteria Desulfovibrio. Annu Rev Microbiol. 1984;38:551–592. doi: 10.1146/annurev.mi.38.100184.003003. [DOI] [PubMed] [Google Scholar]
- Sato Y., Noji S., Suzuki R., Taniguchi S. Dual mechanism for stimulation of glutamate transport by potassium ions in Streptococcus mutans. J Bacteriol. 1989 Sep;171(9):4963–4966. doi: 10.1128/jb.171.9.4963-4966.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vasconcelos I., Girbal L., Soucaille P. Regulation of carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH on mixtures of glucose and glycerol. J Bacteriol. 1994 Mar;176(5):1443–1450. doi: 10.1128/jb.176.5.1443-1450.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]