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. 1994 Oct;176(20):6410–6413. doi: 10.1128/jb.176.20.6410-6413.1994

Gene fusion analysis of membrane protein topology: a direct comparison of alkaline phosphatase and beta-lactamase fusions.

W A Prinz 1, J Beckwith 1
PMCID: PMC196986  PMID: 7929016

Abstract

To compare two approaches to analyzing membrane protein topology, a number of alkaline phosphatase fusions to membrane proteins were converted to beta-lactamase fusions. While some alkaline phosphatase fusions near the N terminus of cytoplasmic loops of membrane proteins have anomalously high levels of activity, the equivalent beta-lactamase fusions do not. This disparity may reflect differences in the folding of beta-lactamase and alkaline phosphatase in the cytoplasm.

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Selected References

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