Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Nov;176(21):6776–6780. doi: 10.1128/jb.176.21.6776-6780.1994

A physical map of the hyperthermophilic bacterium Aquifex pyrophilus chromosome.

Z Shao 1, W Mages 1, R Schmitt 1
PMCID: PMC197038  PMID: 7961434

Abstract

A genomic map of the hyperthermophilic hydrogen-oxidizing bacterium Aquifex pyrophilus was established with NotI (GC/GGCCGC), SpeI (A/CTAGT), and XbaI (T/CTAGA). Linking clones and cross-hybridization of restriction fragments revealed a single circular chromosome of 1.6 Mbp. A single flagellin gene and six rRNA gene units were located on this map by Southern hybridization.

Full text

PDF
6776

Images in this article

6776Image
on p.6776
6778Image
on p.6778

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashby M. K., Bergquist P. L. Cloning and sequence of IS1000, a putative insertion sequence from Thermus thermophilus HB8. Plasmid. 1990 Jul;24(1):1–11. doi: 10.1016/0147-619x(90)90020-d. [DOI] [PubMed] [Google Scholar]
  2. Borges K. M., Bergquist P. L. Genomic restriction map of the extremely thermophilic bacterium Thermus thermophilus HB8. J Bacteriol. 1993 Jan;175(1):103–110. doi: 10.1128/jb.175.1.103-110.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Burggraf S., Olsen G. J., Stetter K. O., Woese C. R. A phylogenetic analysis of Aquifex pyrophilus. Syst Appl Microbiol. 1992 Aug;15(3):352–356. doi: 10.1016/S0723-2020(11)80207-9. [DOI] [PubMed] [Google Scholar]
  4. Chen H., Keseler I. M., Shimkets L. J. Genome size of Myxococcus xanthus determined by pulsed-field gel electrophoresis. J Bacteriol. 1990 Aug;172(8):4206–4213. doi: 10.1128/jb.172.8.4206-4213.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chu G., Vollrath D., Davis R. W. Separation of large DNA molecules by contour-clamped homogeneous electric fields. Science. 1986 Dec 19;234(4783):1582–1585. doi: 10.1126/science.3538420. [DOI] [PubMed] [Google Scholar]
  6. Kieser H. M., Kieser T., Hopwood D. A. A combined genetic and physical map of the Streptomyces coelicolor A3(2) chromosome. J Bacteriol. 1992 Sep;174(17):5496–5507. doi: 10.1128/jb.174.17.5496-5507.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kim C. W., Markiewicz P., Lee J. J., Schierle C. F., Miller J. H. Studies of the hyperthermophile Thermotoga maritima by random sequencing of cDNA and genomic libraries. Identification and sequencing of the trpEG (D) operon. J Mol Biol. 1993 Jun 20;231(4):960–981. doi: 10.1006/jmbi.1993.1345. [DOI] [PubMed] [Google Scholar]
  8. Le Bourgeois P., Mata M., Ritzenthaler P. Genome comparison of Lactococcus strains by pulsed-field gel electrophoresis. FEMS Microbiol Lett. 1989 May;50(1-2):65–69. doi: 10.1016/0378-1097(89)90460-6. [DOI] [PubMed] [Google Scholar]
  9. Leblond P., Demuyter P., Simonet J. M., Decaris B. Genetic instability and associated genome plasticity in Streptomyces ambofaciens: pulsed-field gel electrophoresis evidence for large DNA alterations in a limited genomic region. J Bacteriol. 1991 Jul;173(13):4229–4233. doi: 10.1128/jb.173.13.4229-4233.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Liu S. L., Hessel A., Sanderson K. E. Genomic mapping with I-Ceu I, an intron-encoded endonuclease specific for genes for ribosomal RNA, in Salmonella spp., Escherichia coli, and other bacteria. Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6874–6878. doi: 10.1073/pnas.90.14.6874. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Marshall P., Lemieux C. The I-CeuI endonuclease recognizes a sequence of 19 base pairs and preferentially cleaves the coding strand of the Chlamydomonas moewusii chloroplast large subunit rRNA gene. Nucleic Acids Res. 1992 Dec 11;20(23):6401–6407. doi: 10.1093/nar/20.23.6401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McClelland M., Bhagwat A. S. Biased DNA repair. Nature. 1992 Feb 13;355(6361):595–596. doi: 10.1038/355595b0. [DOI] [PubMed] [Google Scholar]
  13. McClelland M., Jones R., Patel Y., Nelson M. Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucleic Acids Res. 1987 Aug 11;15(15):5985–6005. doi: 10.1093/nar/15.15.5985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Noll K. M. Chromosome map of the thermophilic archaebacterium Thermococcus celer. J Bacteriol. 1989 Dec;171(12):6720–6725. doi: 10.1128/jb.171.12.6720-6725.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schmid K., Thomm M., Laminet A., Laue F. G., Kessler C., Stetter K. O., Schmitt R. Three new restriction endonucleases MaeI, MaeII and MaeIII from Methanococcus aeolicus. Nucleic Acids Res. 1984 Mar 26;12(6):2619–2628. doi: 10.1093/nar/12.6.2619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Smith C. L., Condemine G. New approaches for physical mapping of small genomes. J Bacteriol. 1990 Mar;172(3):1167–1172. doi: 10.1128/jb.172.3.1167-1172.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smith C. L., Econome J. G., Schutt A., Klco S., Cantor C. R. A physical map of the Escherichia coli K12 genome. Science. 1987 Jun 12;236(4807):1448–1453. doi: 10.1126/science.3296194. [DOI] [PubMed] [Google Scholar]
  18. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES