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. 1990 Sep;62(3):368–371. doi: 10.1038/bjc.1990.299

Enhancement by neurotensin of experimental carcinogenesis induced in rat colon by azoxymethane.

M Tasuta 1, H Iishi 1, M Baba 1, H Taniguchi 1
PMCID: PMC1971434  PMID: 2206944

Abstract

The effects of neurotensin on the incidence, number, size, and histology of colon tumours induced by azoxymethane (AOM) were investigated in Wistar rats. Rats were given 10 weekly injections of AOM (7.4 mg kg-1 body weight) and were also given 200 micrograms kg-1 of neurotensin in depot form every other day until the end of the experiment. In week 40, prolonged alternate-day administration of neurotensin resulted in significant increases in the number and size of colon tumours and the incidence of adenocarcinomas penetrating muscle layer and deeper. However, neurotensin did not influence the incidence of tumour-bearing rats and the histological appearance of colon tumours. Administration of neurotensin caused a significant increase in the labelling index of the colon cancers but not that of colon mucosa. These findings indicate that neurotensin enhanced the growth of colon tumours, possibly related to its effect in increasing proliferation of colon cancer cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amar S., Kitabgi P., Vincent J. P. Activation of phosphatidylinositol turnover by neurotensin receptors in the human colonic adenocarcinoma cell line HT29. FEBS Lett. 1986 May 26;201(1):31–36. doi: 10.1016/0014-5793(86)80565-8. [DOI] [PubMed] [Google Scholar]
  2. Amar S., Mazella J., Checler F., Kitabgi P., Vincent J. P. Regulation of cyclic GMP levels by neurotensin in neuroblastoma clone N1E115. Biochem Biophys Res Commun. 1985 May 31;129(1):117–125. doi: 10.1016/0006-291x(85)91411-1. [DOI] [PubMed] [Google Scholar]
  3. Carraway R., Kitabgi P., Leeman S. E. The amino acid sequence of radioimmunoassayable neurotensin from bovine intestine. J Biol Chem. 1978 Nov 25;253(22):7996–7998. [PubMed] [Google Scholar]
  4. Carraway R., Leeman S. E. The amino acid sequence of a hypothalamic peptide, neurotensin. J Biol Chem. 1975 Mar 10;250(5):1907–1911. [PubMed] [Google Scholar]
  5. DeRubertis F. R., Chayoth R., Field J. B. The content and metabolism of cyclic adenosine 3', 5'-monophosphate and cyclic guanosine 3', 5'-monophosphate in adenocarcinoma of the human colon. J Clin Invest. 1976 Mar;57(3):641–649. doi: 10.1172/JCI108320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Feurle G. E., Müller B., Rix E. Neurotensin induces hyperplasia of the pancreas and growth of the gastric antrum in rats. Gut. 1987;28 (Suppl):19–23. doi: 10.1136/gut.28.suppl.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gratzner H. G. Monoclonal antibody to 5-bromo- and 5-iododeoxyuridine: A new reagent for detection of DNA replication. Science. 1982 Oct 29;218(4571):474–475. doi: 10.1126/science.7123245. [DOI] [PubMed] [Google Scholar]
  8. Iinuma K., Ikeda I., Takai M., Yanagawa Y., Kurata K. [Gastrin radioimmunoassay with polyethylene glycol method]. Radioisotopes. 1982 Jul;31(7):350–356. [PubMed] [Google Scholar]
  9. Kerr J. F., Searle J. Deletion of cells by apoptosis during castration-induced involution of the rat prostate. Virchows Arch B Cell Pathol. 1973 Jun 25;13(2):87–102. doi: 10.1007/BF02889300. [DOI] [PubMed] [Google Scholar]
  10. McGregor D. B., Jones R. D., Karlin D. A., Romsdahl M. M. Trophic effects of gastrin on colorectal neoplasms in the rat. Ann Surg. 1982 Feb;195(2):219–223. doi: 10.1097/00000658-198202000-00017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morstyn G., Hsu S. M., Kinsella T., Gratzner H., Russo A., Mitchell J. B. Bromodeoxyuridine in tumors and chromosomes detected with a monoclonal antibody. J Clin Invest. 1983 Nov;72(5):1844–1850. doi: 10.1172/JCI111145. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Polak J. M., Sullivan S. N., Bloom S. R., Buchan A. M., Facer P., Brown M. R., Pearse A. G. Specific localisation of neurotensin to the N cell in human intestine by radioimmunoassay and immunocytochemistry. Nature. 1977 Nov 10;270(5633):183–184. doi: 10.1038/270183a0. [DOI] [PubMed] [Google Scholar]
  13. Stefanini M., De Martino C., Zamboni L. Fixation of ejaculated spermatozoa for electron microscopy. Nature. 1967 Oct 14;216(5111):173–174. doi: 10.1038/216173a0. [DOI] [PubMed] [Google Scholar]
  14. Sunter J. P., Appleton D. R., Wright N. A., Watson A. J. Pathological features of the colonic tumours induced in rats by the administration of 1,2-dimethylhydrazine. Virchows Arch B Cell Pathol. 1978 Dec 21;29(3):211–223. doi: 10.1007/BF02899354. [DOI] [PubMed] [Google Scholar]
  15. Tatsuta M., Iishi H., Baba M., Taniguchi H. Promotion by neurotensin of gastric carcinogenesis induced by N-methyl-N'-nitro-N-nitrosoguanidine in Wistar rats. Cancer Res. 1989 Feb 15;49(4):843–846. [PubMed] [Google Scholar]
  16. Tatsuta M., Itoh T., Okuda S., Tamura H., Yamamura H. Effect of fundusectomy on serum and antral gastrin levels in rats. Gastroenterology. 1977 Jan;72(1):78–81. [PubMed] [Google Scholar]
  17. Terada N., Yamamoto R., Takada T., Miyake T., Terakawa N., Wakimoto H., Taniguchi H., Li W., Kitamura Y., Matsumoto K. Inhibitory effect of progesterone on cell death of mouse uterine epithelium. J Steroid Biochem. 1989 Dec;33(6):1091–1096. doi: 10.1016/0022-4731(89)90414-7. [DOI] [PubMed] [Google Scholar]
  18. Wood J. G., Hoang H. D., Bussjaeger L. J., Solomon T. E. Neurotensin stimulates growth of small intestine in rats. Am J Physiol. 1988 Dec;255(6 Pt 1):G813–G817. doi: 10.1152/ajpgi.1988.255.6.G813. [DOI] [PubMed] [Google Scholar]

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