Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1991 Feb;63(2):298–302. doi: 10.1038/bjc.1991.69

DNA flow cytometry of hereditary and sporadic paragangliomas (glomus tumours).

A G van der Mey 1, C J Cornelisse 1, J Hermans 1, J L Terpstra 1, P H Schmidt 1, G J Fleuren 1
PMCID: PMC1971763  PMID: 1997109

Abstract

Paragangliomas (glomus tumours) are benign, hypervascular tumours which in general are treated by surgical excision. The indication for treatment of these often slow-growing tumours needs additional criteria for predicting tumour progressiveness. For this reason the nuclear DNA content of 99 paragangliomas, 65 of them originating from patients with a positive family history, was analysed by flow cytometry. Unequivocal evidence of DNA aneuploidy was found in 37% of these clinically and histologically benign tumours, the average duration of follow up amounting to at least 10 years. The DNA index of the aneuploid tumours ranged from 0.90 to 2.03. No correlation was found between DNA ploidy and familiality or between DNA content and clinical criteria indicative of tumour progression, which means that DNA ploidy of these tumours cannot serve as a predictor for an expected growth pattern or familiality. DNA aneuploidy in hereditary and sporadic paragangliomas is not clinically related to malignancy, but indicates that these tumours are true neoplasias cytogenetically.

Full text

PDF
298

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anniko M., Tribukait B., Wersäll J. DNA ploidy and cell phase in human pituitary tumors. Cancer. 1984 Apr 15;53(8):1708–1713. doi: 10.1002/1097-0142(19840415)53:8<1708::aid-cncr2820530815>3.0.co;2-l. [DOI] [PubMed] [Google Scholar]
  2. Arias-Stella J., Valcarcel J. Chief cell hyperplasia in the human carotid body at high altitudes; physiologic and pathologic significance. Hum Pathol. 1976 Jul;7(4):361–373. doi: 10.1016/s0046-8177(76)80052-4. [DOI] [PubMed] [Google Scholar]
  3. Barnes L., Taylor S. R. Carotid body paragangliomas. A clinicopathologic and DNA analysis of 13 tumors. Arch Otolaryngol Head Neck Surg. 1990 Apr;116(4):447–453. doi: 10.1001/archotol.1990.01870040069016. [DOI] [PubMed] [Google Scholar]
  4. Chedid A., Jao W. Hereditary tumors of the carotid bodies and chronic obstructive pulmonary disease. Cancer. 1974 Jun;33(6):1635–1641. doi: 10.1002/1097-0142(197406)33:6<1635::aid-cncr2820330625>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  5. Cornelisse C. J., van de Velde C. J., Caspers R. J., Moolenaar A. J., Hermans J. DNA ploidy and survival in breast cancer patients. Cytometry. 1987 Mar;8(2):225–234. doi: 10.1002/cyto.990080217. [DOI] [PubMed] [Google Scholar]
  6. Grimley P. M., Glenner G. G. Histology and ultrastructure of carotid body paragangliomas. Comparison with the normal gland. Cancer. 1967 Sep;20(9):1473–1488. doi: 10.1002/1097-0142(196709)20:9<1473::aid-cncr2820200914>3.0.co;2-i. [DOI] [PubMed] [Google Scholar]
  7. Hedley D. W., Friedlander M. L., Taylor I. W., Rugg C. A., Musgrove E. A. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983 Nov;31(11):1333–1335. doi: 10.1177/31.11.6619538. [DOI] [PubMed] [Google Scholar]
  8. Joensuu H., Klemi P. J. DNA aneuploidy in adenomas of endocrine organs. Am J Pathol. 1988 Jul;132(1):145–151. [PMC free article] [PubMed] [Google Scholar]
  9. Koss L. G., Czerniak B., Herz F., Wersto R. P. Flow cytometric measurements of DNA and other cell components in human tumors: a critical appraisal. Hum Pathol. 1989 Jun;20(6):528–548. doi: 10.1016/0046-8177(89)90244-x. [DOI] [PubMed] [Google Scholar]
  10. Lack E. E., Cubilla A. L., Woodruff J. M. Paragangliomas of the head and neck region. A pathologic study of tumors from 71 patients. Hum Pathol. 1979 Mar;10(2):191–218. doi: 10.1016/s0046-8177(79)80008-8. [DOI] [PubMed] [Google Scholar]
  11. Merkel D. E., McGuire W. L. Ploidy, proliferative activity and prognosis. DNA flow cytometry of solid tumors. Cancer. 1990 Mar 1;65(5):1194–1205. doi: 10.1002/1097-0142(19900301)65:5<1194::aid-cncr2820650528>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  12. Reubi J. C., Torhorst J. The relationship between somatostatin, epidermal growth factor, and steroid hormone receptors in breast cancer. Cancer. 1989 Sep 15;64(6):1254–1260. doi: 10.1002/1097-0142(19890915)64:6<1254::aid-cncr2820640615>3.0.co;2-d. [DOI] [PubMed] [Google Scholar]
  13. Rodenburg C. J., Cornelisse C. J., Heintz P. A., Hermans J., Fleuren G. J. Tumor ploidy as a major prognostic factor in advanced ovarian cancer. Cancer. 1987 Jan 15;59(2):317–323. doi: 10.1002/1097-0142(19870115)59:2<317::aid-cncr2820590225>3.0.co;2-4. [DOI] [PubMed] [Google Scholar]
  14. Rosenwasser H. Long-term results of therapy of glomus jugulare tumors. Arch Otolaryngol. 1973 Jan;97(1):49–54. doi: 10.1001/archotol.1973.00780010053013. [DOI] [PubMed] [Google Scholar]
  15. Saldana M. J., Salem L. E., Travezan R. High altitude hypoxia and chemodectomas. Hum Pathol. 1973 Jun;4(2):251–263. doi: 10.1016/s0046-8177(73)80012-7. [DOI] [PubMed] [Google Scholar]
  16. Schwartz D., Banner B. F., Roseman D. L., Coon J. S. Origin of multiple "primary" colon carcinomas. A retrospective flow cytometric study. Cancer. 1986 Nov 1;58(9):2082–2088. doi: 10.1002/1097-0142(19861101)58:9<2082::aid-cncr2820580920>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  17. Sciallero S., Bruno S., Di Vinci A., Geido E., Aste H., Giaretti W. Flow cytometric DNA ploidy in colorectal adenomas and family history of colorectal cancer. Cancer. 1988 Jan 1;61(1):114–120. doi: 10.1002/1097-0142(19880101)61:1<114::aid-cncr2820610120>3.0.co;2-i. [DOI] [PubMed] [Google Scholar]
  18. Shackney S. E., Smith C. A., Miller B. W., Burholt D. R., Murtha K., Giles H. R., Ketterer D. M., Pollice A. A. Model for the genetic evolution of human solid tumors. Cancer Res. 1989 Jun 15;49(12):3344–3354. [PubMed] [Google Scholar]
  19. Stiller D., Katenkamp D., Küttner K. Jugular body tumors: hyperplasias or true neoplasms? Light and electron microscopical investigations. Virchows Arch A Pathol Anat Histol. 1975;365(2):163–177. doi: 10.1007/BF00432387. [DOI] [PubMed] [Google Scholar]
  20. Vindeløv L. L., Christensen I. J., Nissen N. I. A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry. 1983 Mar;3(5):323–327. doi: 10.1002/cyto.990030503. [DOI] [PubMed] [Google Scholar]
  21. van Gils A. P., van der Mey A. G., Hoogma R. P., Falke T. H., Moolenaar A. J., Pauwels E. K., van Kroonenburgh M. J. Iodine-123-metaiodobenzylguanidine scintigraphy in patients with chemodectomas of the head and neck region. J Nucl Med. 1990 Jul;31(7):1147–1155. [PubMed] [Google Scholar]
  22. van den Ingh H. F., Griffioen G., Cornelisse C. J. Flow cytometric detection of aneuploidy in colorectal adenomas. Cancer Res. 1985 Jul;45(7):3392–3397. [PubMed] [Google Scholar]
  23. van der Mey A. G., Maaswinkel-Mooy P. D., Cornelisse C. J., Schmidt P. H., van de Kamp J. J. Genomic imprinting in hereditary glomus tumours: evidence for new genetic theory. Lancet. 1989 Dec 2;2(8675):1291–1294. doi: 10.1016/s0140-6736(89)91908-9. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES