Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1991 Mar;63(3):367–371. doi: 10.1038/bjc.1991.87

Increased levels of type VIII collagen in human brain tumours compared to normal brain tissue and non-neoplastic cerebral disorders.

W Paulus 1, E H Sage 1, U Liszka 1, M L Iruela-Arispe 1, K Jellinger 1
PMCID: PMC1971869  PMID: 2003978

Abstract

The expression of type VIII collagen was examined in the normal and diseased human brain. Focal immunoreactivity was seen in histologically abnormal vessels of all four angiomas and 40 of 52 brain tumours (gliomas, meningiomas and schwannomas). An extended staining pattern, as well as a punctate distribution, was frequently observed in affected vessels. Staining was not apparent in nine normal brains and in 15 pathologic brains showing various cerebrovascular abnormalities, including Alzheimer's, Leigh's and Wernicke's diseases. Immunoblotting of glioblastomas revealed two bands at 56 kD and 67 kD which were also present at low levels in normal frontal cortex. The extracellular distribution of type VIII collagen was different from that of the other collagen types which have been described in brain and resembles patterns of expression described for certain tissues during mammalian embryogenesis (Kapoor et al., 1988). Our results provide additional evidence for the participation of type VIII collagen in some types of angiogenesis.

Full text

PDF
367

Images in this article

368Figure 1
on p.368
368Figure 2
on p.368
369Figure 3
on p.369
369Figure 4
on p.369
369Figure 5
on p.369
369Figure 6
on p.369
369Figure 7
on p.369
369Figure 8
on p.369
370Figure 9
on p.370

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alitalo K., Bornstein P., Vaheri A., Sage H. Biosynthesis of an unusual collagen type by human astrocytoma cells in vitro. J Biol Chem. 1983 Feb 25;258(4):2653–2661. [PubMed] [Google Scholar]
  2. Bellon G., Caulet T., Cam Y., Pluot M., Poulin G., Pytlinska M., Bernard M. H. Immunohistochemical localisation of macromolecules of the basement membrane and extracellular matrix of human gliomas and meningiomas. Acta Neuropathol. 1985;66(3):245–252. doi: 10.1007/BF00688590. [DOI] [PubMed] [Google Scholar]
  3. Benya P. D. EC collagen: biosynthesis by corneal endothelial cells and separation from type IV without pepsin treatment or denaturation. Ren Physiol. 1980;3(1-6):30–35. doi: 10.1159/000172738. [DOI] [PubMed] [Google Scholar]
  4. Fischer V. W., Siddiqi A., Yusufaly Y. Altered angioarchitecture in selected areas of brains with Alzheimer's disease. Acta Neuropathol. 1990;79(6):672–679. doi: 10.1007/BF00294246. [DOI] [PubMed] [Google Scholar]
  5. Hirano A., Matsui T. Vascular structures in brain tumors. Hum Pathol. 1975 Sep;6(5):611–621. doi: 10.1016/s0046-8177(75)80045-1. [DOI] [PubMed] [Google Scholar]
  6. Kapoor R., Bornstein P., Sage E. H. Type VIII collagen from bovine Descemet's membrane: structural characterization of a triple-helical domain. Biochemistry. 1986 Jul 1;25(13):3930–3937. doi: 10.1021/bi00361a029. [DOI] [PubMed] [Google Scholar]
  7. Kapoor R., Sakai L. Y., Funk S., Roux E., Bornstein P., Sage E. H. Type VIII collagen has a restricted distribution in specialized extracellular matrices. J Cell Biol. 1988 Aug;107(2):721–730. doi: 10.1083/jcb.107.2.721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Labermeier U., Kenney M. C. The presence of EC collagen and type IV collagen in bovine Descemet's membranes. Biochem Biophys Res Commun. 1983 Oct 31;116(2):619–625. doi: 10.1016/0006-291x(83)90569-7. [DOI] [PubMed] [Google Scholar]
  9. McComb R. D., Moul J. M., Bigner D. D. Distribution of type VI collagen in human gliomas: comparison with fibronectin and glioma-mesenchymal matrix glycoprotein. J Neuropathol Exp Neurol. 1987 Nov;46(6):623–633. doi: 10.1097/00005072-198711000-00002. [DOI] [PubMed] [Google Scholar]
  10. Oda Y., Kawahara E., Minamoto T., Ueda Y., Ikeda K., Nagai Y., Nakanishi I. Immunohistochemical studies on the tissue localization of collagen types I, III, IV, V and VI in schwannomas. Correlation with ultrastructural features of the extracellular matrix. Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(3):153–163. doi: 10.1007/BF02890013. [DOI] [PubMed] [Google Scholar]
  11. Ostergaard J. R., Oxlund H. Collagen type III deficiency in patients with rupture of intracranial saccular aneurysms. J Neurosurg. 1987 Nov;67(5):690–696. doi: 10.3171/jns.1987.67.5.0690. [DOI] [PubMed] [Google Scholar]
  12. Paulus W., Roggendorf W., Schuppan D. Immunohistochemical investigation of collagen subtypes in human glioblastomas. Virchows Arch A Pathol Anat Histopathol. 1988;413(4):325–332. doi: 10.1007/BF00783025. [DOI] [PubMed] [Google Scholar]
  13. Roggendorf W., Opitz H., Schuppan D. Altered expression of collagen type VI in brain vessels of patients with chronic hypertension. A comparison with the distribution of collagen IV and procollagen III. Acta Neuropathol. 1988;77(1):55–60. doi: 10.1007/BF00688243. [DOI] [PubMed] [Google Scholar]
  14. Rutka J. T., Apodaca G., Stern R., Rosenblum M. The extracellular matrix of the central and peripheral nervous systems: structure and function. J Neurosurg. 1988 Aug;69(2):155–170. doi: 10.3171/jns.1988.69.2.0155. [DOI] [PubMed] [Google Scholar]
  15. Rutka J. T., Myatt C. A., Giblin J. R., Davis R. L., Rosenblum M. L. Distribution of extracellular matrix proteins in primary human brain tumours: an immunohistochemical analysis. Can J Neurol Sci. 1987 Feb;14(1):25–30. doi: 10.1017/s0317167100026123. [DOI] [PubMed] [Google Scholar]
  16. Sage H., Balian G., Vogel A. M., Bornstein P. Type VIII collagen. Synthesis by normal and malignant cells in culture. Lab Invest. 1984 Feb;50(2):219–231. [PubMed] [Google Scholar]
  17. Sage H., Iruela-Arispe M. L. Type VIII collagen in murine development. Association with capillary formation in vitro. Ann N Y Acad Sci. 1990;580:17–31. doi: 10.1111/j.1749-6632.1990.tb17914.x. [DOI] [PubMed] [Google Scholar]
  18. Sage H., Pritzl P., Bornstein P. A unique, pepsin-sensitive collagen synthesized by aortic endothelial cells in culture. Biochemistry. 1980 Dec 9;19(25):5747–5755. doi: 10.1021/bi00566a013. [DOI] [PubMed] [Google Scholar]
  19. Sawada H., Konomi H., Hirosawa K. Characterization of the collagen in the hexagonal lattice of Descemet's membrane: its relation to type VIII collagen. J Cell Biol. 1990 Jan;110(1):219–227. doi: 10.1083/jcb.110.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schiffer D., Chiò A., Giordana M. T., Mauro A., Migheli A., Vigliani M. C. The vascular response to tumor infiltration in malignant gliomas. Morphometric and reconstruction study. Acta Neuropathol. 1989;77(4):369–378. doi: 10.1007/BF00687371. [DOI] [PubMed] [Google Scholar]
  21. Yamaguchi N., Benya P. D., van der Rest M., Ninomiya Y. The cloning and sequencing of alpha 1(VIII) collagen cDNAs demonstrate that type VIII collagen is a short chain collagen and contains triple-helical and carboxyl-terminal non-triple-helical domains similar to those of type X collagen. J Biol Chem. 1989 Sep 25;264(27):16022–16029. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES