Skip to main content
NCBI home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1994 Dec;176(24):7748–7753. doi: 10.1128/jb.176.24.7748-7753.1994

Cloning of the hsp70 (dnaK) genes from Rhizobium meliloti and Pseudomonas cepacia: phylogenetic analyses of mitochondrial origin based on a highly conserved protein sequence.

M Falah 1, R S Gupta 1
PMCID: PMC197237  PMID: 7528198

Abstract

The genes for hsp70 (or dnaK) have been cloned and sequenced from Rhizobium meliloti and Pseudomonas cepacia, two bacterial species belonging to the alpha- and beta-subdivisions of gram-negative proteobacteria, respectively. On the basis of global alignment of HSP70 proteins, several sequence signatures have been identified that are distinctive of mitochondrial homologs and gram-negative proteobacteria on the one hand and the chloroplasts and cyanobacteria on the other. Detailed phylogenetic analyses of HSP70 sequences from various eubacteria and eukaryotic organellar and cytosolic homologs support the inference regarding the origin of mitochondria from a member of the alpha-proteobacteria and of chloroplasts from cyanobacteria. The analysis presented here also suggests a monophyletic origin of the mitochondrial homologs.

Full text

PDF
7748

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boorstein W. R., Ziegelhoffer T., Craig E. A. Molecular evolution of the HSP70 multigene family. J Mol Evol. 1994 Jan;38(1):1–17. doi: 10.1007/BF00175490. [DOI] [PubMed] [Google Scholar]
  2. Dickerson R. E. Evolution and gene transfer in purple photosynthetic bacteria. Nature. 1980 Jan 10;283(5743):210–212. doi: 10.1038/283210a0. [DOI] [PubMed] [Google Scholar]
  3. Galley K. A., Singh B., Gupta R. S. Cloning of HSP70 (dnaK) gene from Clostridium perfringens using a general polymerase chain reaction based approach. Biochim Biophys Acta. 1992 Mar 24;1130(2):203–208. doi: 10.1016/0167-4781(92)90529-9. [DOI] [PubMed] [Google Scholar]
  4. Gray M. W., Cedergren R., Abel Y., Sankoff D. On the evolutionary origin of the plant mitochondrion and its genome. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2267–2271. doi: 10.1073/pnas.86.7.2267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gray M. W., Doolittle W. F. Has the endosymbiont hypothesis been proven? Microbiol Rev. 1982 Mar;46(1):1–42. doi: 10.1128/mr.46.1.1-42.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gray M. W. Organelle origins and ribosomal RNA. Biochem Cell Biol. 1988 May;66(5):325–348. doi: 10.1139/o88-042. [DOI] [PubMed] [Google Scholar]
  7. Gray M. W. The endosymbiont hypothesis revisited. Int Rev Cytol. 1992;141:233–357. doi: 10.1016/s0074-7696(08)62068-9. [DOI] [PubMed] [Google Scholar]
  8. Gupta R. S., Aitken K., Falah M., Singh B. Cloning of Giardia lamblia heat shock protein HSP70 homologs: implications regarding origin of eukaryotic cells and of endoplasmic reticulum. Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):2895–2899. doi: 10.1073/pnas.91.8.2895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Gupta R. S., Golding G. B. Evolution of HSP70 gene and its implications regarding relationships between archaebacteria, eubacteria, and eukaryotes. J Mol Evol. 1993 Dec;37(6):573–582. doi: 10.1007/BF00182743. [DOI] [PubMed] [Google Scholar]
  10. Gupta R. S., Singh B. Cloning of the HSP70 gene from Halobacterium marismortui: relatedness of archaebacterial HSP70 to its eubacterial homologs and a model for the evolution of the HSP70 gene. J Bacteriol. 1992 Jul;174(14):4594–4605. doi: 10.1128/jb.174.14.4594-4605.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morden C. W., Delwiche C. F., Kuhsel M., Palmer J. D. Gene phylogenies and the endosymbiotic origin of plastids. Biosystems. 1992;28(1-3):75–90. doi: 10.1016/0303-2647(92)90010-v. [DOI] [PubMed] [Google Scholar]
  12. Saitou N., Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987 Jul;4(4):406–425. doi: 10.1093/oxfordjournals.molbev.a040454. [DOI] [PubMed] [Google Scholar]
  13. Schwartz R. M., Dayhoff M. O. Origins of prokaryotes, eukaryotes, mitochondria, and chloroplasts. Science. 1978 Jan 27;199(4327):395–403. doi: 10.1126/science.202030. [DOI] [PubMed] [Google Scholar]
  14. Taylor F. J. An overview of the status of evolutionary cell symbiosis theories. Ann N Y Acad Sci. 1987;503:1–16. doi: 10.1111/j.1749-6632.1987.tb40595.x. [DOI] [PubMed] [Google Scholar]
  15. Woese C. R. Bacterial evolution. Microbiol Rev. 1987 Jun;51(2):221–271. doi: 10.1128/mr.51.2.221-271.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yang D., Oyaizu Y., Oyaizu H., Olsen G. J., Woese C. R. Mitochondrial origins. Proc Natl Acad Sci U S A. 1985 Jul;82(13):4443–4447. doi: 10.1073/pnas.82.13.4443. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES