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. 1991 May;63(5):675–680. doi: 10.1038/bjc.1991.154

Microencapsulation of human cells: its effects on growth of normal and tumour cells in vitro.

S M Shimi 1, D Hopwood 1, E L Newman 1, A Cuschieri 1
PMCID: PMC1972378  PMID: 2039691

Abstract

The growth kinetics of established human colorectal tumour cell lines (HT29, HT115 and COLO 320DM) and human diploid fibroblasts (Flow 2002) were studied in conventional culture and in microcapsules formed from alginate-poly(L-lysine)-alginate membranes. The tumour lines grew rapidly in microcapsules but, in the case of the substrate-adherent lines HT29 and HT115, only after a prolonged lag phase. This phase was reduced by serial passage in microcapsules. The anchorage-independent line COLO 320DM showed no lengthening in lag phase. Microencapsulated fibroblasts underwent negligible growth but remained viable. Some evidence for functional differentiation (microvilli, cell-cell junctions) of the tumour line HT115 within the microcapsules was observed. We conclude that the use of microcapsules provides an alternative system with some advantages for the study of human cancer and its metastases in vitro.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bateman A. E., Peckham M. J., Steel G. G. Assays of drug sensitivity for cells from human tumours: in vitro and in vivo tests on a xenografted tumour. Br J Cancer. 1979 Jul;40(1):81–88. doi: 10.1038/bjc.1979.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chang T. M., MacIntosh F. C., Mason S. G. Semipermeable aqueous microcapsules. I. Preparation and properties. Can J Physiol Pharmacol. 1966 Jan;44(1):115–128. doi: 10.1139/y66-013. [DOI] [PubMed] [Google Scholar]
  3. Chen C. F., Hwang J. M., Jao S. W., Leu F. J., Chen K. Y. Microencapsulation [corrected] of tumor cells and assay for selecting anticancer drugs. Proc Natl Sci Counc Repub China B. 1988 Oct;12(4):252–261. [PubMed] [Google Scholar]
  4. Darquy S., Reach G. Immunoisolation of pancreatic B cells by microencapsulation. An in vitro study. Diabetologia. 1985 Oct;28(10):776–780. doi: 10.1007/BF00265027. [DOI] [PubMed] [Google Scholar]
  5. Edelstein M. B., Smink T., Ruiter D. J., Visser W., van Putten L. M. Improvements and limitations of the subrenal capsule assay for determining tumour sensitivity to cytostatic drugs. Eur J Cancer Clin Oncol. 1984 Dec;20(12):1549–1556. doi: 10.1016/0277-5379(84)90149-4. [DOI] [PubMed] [Google Scholar]
  6. Giovanella B. C., Stehlin J. S., Jr, Shepard R. C., Williams L. J., Jr Correlation between response to chemotherapy of human tumors in patients and in nude mice. Cancer. 1983 Oct 1;52(7):1146–1152. doi: 10.1002/1097-0142(19831001)52:7<1146::aid-cncr2820520704>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  7. Gorelik E., Ovejera A., Shoemaker R., Jarvis A., Alley M., Duff R., Mayo J., Herberman R., Boyd M. Microencapsulated tumor assay: new short-term assay for in vivo evaluation of the effects of anticancer drugs on human tumor cell lines. Cancer Res. 1987 Nov 1;47(21):5739–5747. [PubMed] [Google Scholar]
  8. Lim F., Sun A. M. Microencapsulated islets as bioartificial endocrine pancreas. Science. 1980 Nov 21;210(4472):908–910. doi: 10.1126/science.6776628. [DOI] [PubMed] [Google Scholar]
  9. O'Shea G. M., Sun A. M. Encapsulation of rat islets of Langerhans prolongs xenograft survival in diabetic mice. Diabetes. 1986 Aug;35(8):943–946. doi: 10.2337/diab.35.8.943. [DOI] [PubMed] [Google Scholar]
  10. Selby P., Buick R. N., Tannock I. A critical appraisal of the "human tumor stem-cell assay". N Engl J Med. 1983 Jan 20;308(3):129–134. doi: 10.1056/NEJM198301203080304. [DOI] [PubMed] [Google Scholar]

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