Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1991 Jun;63(6):953–958. doi: 10.1038/bjc.1991.208

Abnormal phosphomonoester signals in 31P MR spectra from patients with hepatic lymphoma. A possible marker of liver infiltration and response to chemotherapy.

R M Dixon 1, P W Angus 1, B Rajagopalan 1, G K Radda 1
PMCID: PMC1972561  PMID: 2069850

Abstract

Hepatic infiltration by lymphoma can be difficult to detect by conventional methods. We have studied 22 patients in vivo 31P magnetic resonance spectroscopy of the liver and compared the results with the clinical staging and assessment of liver involvement by computed tomography (CT), ultrasound (US), and liver function tests (LFTs). We find that the phosphomonoester (PME) to ATP, and the PME to Pi ratios are the best indication of liver involvement as in all the patients with liver involvement apparent on CT or US, these ratios were elevated (greater than 2 s.d. above the control mean). Of the patients with deranged LFTs but normal CT or US, five out of nine showed increased PME/ATP and PME/Pi ratios, and in the patients with normal LFTs and normal CT or US, three out of eight patients had raised PME ratios. Extracts of lymphomatous lymph nodes contain high concentrations of phosphoethanolamine which suggests that this compound is responsible for the increase in the PME peak. Eleven patients were studied again after chemotherapy, and those with initially raised PME/ATP and PME/Pi ratios all showed a decrease in these ratios towards normal. The patients with initially normal ratios showed no changes.

Full text

PDF
953

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bagley C. M., Jr, Roth J. A., Thomas L. B., Devita V. T., Jr Liver biopsy in Hodgkin's disease. Clinicopathologic correlations in 127 patients. Ann Intern Med. 1972 Feb;76(2):219–225. doi: 10.7326/0003-4819-76-2-219. [DOI] [PubMed] [Google Scholar]
  2. Berridge M. J. Inositol lipids and cell proliferation. Biochim Biophys Acta. 1987 Apr 20;907(1):33–45. doi: 10.1016/0304-419x(87)90017-5. [DOI] [PubMed] [Google Scholar]
  3. Corbett R. J., Laptook A. R., Nunnally R. L. The use of the chemical shift of the phosphomonoester P-31 magnetic resonance peak for the determination of intracellular pH in the brains of neonates. Neurology. 1987 Nov;37(11):1771–1779. doi: 10.1212/wnl.37.11.1771. [DOI] [PubMed] [Google Scholar]
  4. Cox I. J., Sargentoni J., Calam J., Bryant D. J., Iles R. A. Four-dimensional phosphorus-31 chemical shift imaging of carcinoid metastases in the liver. NMR Biomed. 1988 Feb;1(1):56–60. doi: 10.1002/nbm.1940010110. [DOI] [PubMed] [Google Scholar]
  5. Cunningham C. C., Malloy C. R., Radda G. K. Effect of fasting and acute ethanol administration on the energy state of in vivo liver as measured by 31P-NMR spectroscopy. Biochim Biophys Acta. 1986 Jan 23;885(1):12–22. doi: 10.1016/0167-4889(86)90033-9. [DOI] [PubMed] [Google Scholar]
  6. Daly P. F., Lyon R. C., Straka E. J., Cohen J. S. 31P-NMR spectroscopy of human cancer cells proliferating in a basement membrane gel. FASEB J. 1988 Jul;2(10):2596–2604. doi: 10.1096/fasebj.2.10.3384239. [DOI] [PubMed] [Google Scholar]
  7. Evanochko W. T., Sakai T. T., Ng T. C., Krishna N. R., Kim H. D., Zeidler R. B., Ghanta V. K., Brockman R. W., Schiffer L. M., Braunschweiger P. G. NMR study of in vivo RIF-1 tumors. Analysis of perchloric acid extracts and identification of 1H, 31P and 13C resonances. Biochim Biophys Acta. 1984 Sep 14;805(1):104–116. doi: 10.1016/0167-4889(84)90042-9. [DOI] [PubMed] [Google Scholar]
  8. George T. P., Morash S. C., Cook H. W., Byers D. M., Palmer F. B., Spence M. W. Phosphatidylcholine biosynthesis in cultured glioma cells: evidence for channeling of intermediates. Biochim Biophys Acta. 1989 Aug 22;1004(3):283–291. doi: 10.1016/0005-2760(89)90075-1. [DOI] [PubMed] [Google Scholar]
  9. Glaholm J., Leach M. O., Collins D. J., Mansi J., Sharp J. C., Madden A., Smith I. E., McCready V. R. In-vivo 31P magnetic resonance spectroscopy for monitoring treatment response in breast cancer. Lancet. 1989 Jun 10;1(8650):1326–1327. doi: 10.1016/s0140-6736(89)92717-7. [DOI] [PubMed] [Google Scholar]
  10. Glazer G. M., Smith S. R., Chenevert T. L., Martin P. A., Stevens A. N., Edwards R. H. Image localized 31P magnetic resonance spectroscopy of the human liver. NMR Biomed. 1989 Apr;1(4):184–189. doi: 10.1002/nbm.1940010406. [DOI] [PubMed] [Google Scholar]
  11. Goffinet D. R., Warnke R., Dunnick N. R., Castellino R., Glatstein E., Nelsen T. S., Dorfman R. F., Rosenberg S. A., Kaplan H. S. Clinical and surgical (laparotomy) evaluation of patients with non-Hodgkin's lymphomas. Cancer Treat Rep. 1977 Sep;61(6):981–992. [PubMed] [Google Scholar]
  12. Golding S. J. Use of imaging in the management of lymphoma. Br J Hosp Med. 1989 Feb;41(2):152-4, 156-7. [PubMed] [Google Scholar]
  13. Groener J. E., Klein W., Van Golde L. M. The effect of fasting and refeeding on the composition and synthesis of triacylglycerols, phosphatidylcholines, and phosphatidylethanolamines in rat liver. Arch Biochem Biophys. 1979 Nov;198(1):287–295. doi: 10.1016/0003-9861(79)90421-1. [DOI] [PubMed] [Google Scholar]
  14. Guy G. R., Murray A. W. Tumor promoter stimulation of phosphatidylcholine turnover in HeLa cells. Cancer Res. 1982 May;42(5):1980–1985. [PubMed] [Google Scholar]
  15. Maris J. M., Evans A. E., McLaughlin A. C., D'Angio G. J., Bolinger L., Manos H., Chance B. 31P nuclear magnetic resonance spectroscopic investigation of human neuroblastoma in situ. N Engl J Med. 1985 Jun 6;312(23):1500–1505. doi: 10.1056/NEJM198506063122307. [DOI] [PubMed] [Google Scholar]
  16. Miceli M. V., Kan L. S., Newsome D. A. Phosphorus-31 nuclear magnetic resonance spectroscopy of human retinoblastoma cells: correlation with metabolic indices. Biochim Biophys Acta. 1988 Jul 29;970(3):262–269. doi: 10.1016/0167-4889(88)90125-5. [DOI] [PubMed] [Google Scholar]
  17. Navon G., Navon R., Shulman R. G., Yamane T. Phosphate metabolites in lymphoid, Friend erythroleukemia, and HeLa cells observed by high-resolution 31P nuclear magnetic resonance. Proc Natl Acad Sci U S A. 1978 Feb;75(2):891–895. doi: 10.1073/pnas.75.2.891. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Ng T. C., Grundfest S., Vijayakumar S., Baldwin N. J., Majors A. W., Karalis I., Meaney T. F., Shin K. H., Thomas F. J., Tubbs R. Therapeutic response of breast carcinoma monitored by 31P MRS in situ. Magn Reson Med. 1989 Apr;10(1):125–134. doi: 10.1002/mrm.1910100112. [DOI] [PubMed] [Google Scholar]
  19. Oberhaensli R., Rajagopalan B., Galloway G. J., Taylor D. J., Radda G. K. Study of human liver disease with P-31 magnetic resonance spectroscopy. Gut. 1990 Apr;31(4):463–467. doi: 10.1136/gut.31.4.463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Somers R., Burgers J. M., Qasim M., Van Glabbeke M., Duez N., Hayat M. EORTC trial non-Hodgkin lymphomas. Eur J Cancer Clin Oncol. 1987 Mar;23(3):283–293. doi: 10.1016/0277-5379(87)90072-1. [DOI] [PubMed] [Google Scholar]
  21. Styles P. Passive electrical isolation of double coil probes for localized spectroscopy and imaging. NMR Biomed. 1988 Apr;1(2):61–66. doi: 10.1002/nbm.1940010202. [DOI] [PubMed] [Google Scholar]
  22. Sundler R., Akesson B. Biosynthesis of phosphatidylethanolamines and phosphatidylcholines from ethanolamine and choline in rat liver. Biochem J. 1975 Feb;146(2):309–315. doi: 10.1042/bj1460309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sundler R., Akesson B. Regulation of phospholipid biosynthesis in isolated rat hepatocytes. Effect of different substrates. J Biol Chem. 1975 May 10;250(9):3359–3367. [PubMed] [Google Scholar]
  24. Trewby P. N., Portmann B., Brinkley D. M., Williams R. Liver disease as presenting manifestation of Hodgkin's disease. Q J Med. 1979 Jan;48(189):137–150. [PubMed] [Google Scholar]
  25. Weinreb J. C., Brateman L., Maravilla K. R. Magnetic resonance imaging of hepatic lymphoma. AJR Am J Roentgenol. 1984 Dec;143(6):1211–1214. doi: 10.2214/ajr.143.6.1211. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES