Fig. 6.

Beta cell transcription factors and pancreatic hormones expressed in transduced rat livers. a RT-PCR analysis of transcription of the genes encoding EGFP or INS-FUR in animals transduced with pHR’CMVGFP or HMD/INS-FUR LV, respectively: liver (lane 1), pancreas (lane 2), spleen (lane 3), kidney (lane 4) and brain (lane 5) at 60 days. Results for HMD/INS-FUR transduced animals at 500 days were identical (not shown). b RT-PCR analysis of transcription of the genes for: (1) beta cell transcription factors (Pdx1, Neurod1, Neurog3, Nkx2-2, Nkx6-1, Pax4, Pax6); (2) the pancreatic endocrine hormones somatostatin (Sst), glucagon (Gcg) and pancreatic polypeptide (Ppy); (3) GLUT2 (Slc2a2) and glucokinase (Gck); (4) rat insulin 1 and 2 (Ins1, Ins2); (5) insulin proconvertase PC2 (Pc2); (6) the exocrine marker p48 (P48); and (7) β-actin (Actb) in normal liver (lane 1), liver transduced with HMD alone at 60 days (lane 2), liver transduced with HMD/INS-FUR LV at 60 days (lane 3) and 500 days (lane 4), and normal pancreas (lane 5). c Western blot analysis for PDX1 and NEUROD1 in normal pancreas (lane 1), liver transduced with HMD/INS-FUR LV at 60 days (lane 2), liver transduced with HMD alone at 60 days (lane 3) and normal liver (lane 4). PDX1 and NEUROD1 generated bands at 43 and 50 kDa, respectively