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. 2007 May 31;110(6):1887–1894. doi: 10.1182/blood-2007-04-083329

Figure 4.

Figure 4

Binding of denatured VWF to ADAMTS13 and C-terminal truncated variants. Purified VWF pretreated for 2 hours with 1.5 M guanidine-HCl at 37°C was diluted (1:10) with HBS-T buffer with (not shown) or without EDTA into various concentrations (0 to 125 μg/mL or 0 to 500 nM). The diluted VWF was then injected at 20 μL/min for 3 minutes over the CM5 chips covalently coupled by FL-A13 (A), delCUB (B), and MDTCS (C). After the equilibrium was established, the HBS-T buffer without VWF was flowed over the surface to allow the dissociation phase to be recorded. The equilibrium constant, KD, was determined similarly as described for Figure 3. The entries in panel D represent the means (± SD) of 6 repeats.