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. 1984 Jan;49(1):65–71. doi: 10.1038/bjc.1984.10

The nature of the ESR signal in lyophilized tissue and its relevance to malignancy.

N J Dodd, H M Swartz
PMCID: PMC1976665  PMID: 6318790

Abstract

Comparison of 9 and 35 GHz spectra, obtained from frozen and lyophilized tissues, with those from model systems containing ascorbic acid, confirm that the major component of the "lyophilization signal" of tissue is the ascorbyl radical, stabilized by adsorption on an inert matrix. The magnitude of the signal under anoxic conditions is shown to be a measure of cellular damage, which allows intracellular ascorbic acid to be oxidized. On exposure of lyophilized samples to air, the signal increases due to autoxidation of the available tissue ascorbic acid. Under moist atmospheric conditions the ascorbyl radicals readily decay, leaving other radicals, which appear to be formed by interaction of ascorbic acid or ascorbyl radicals with some tissue component. The results show that, although widely studied, the free radical ESR signal of lyophilized tissue is not unique to tumour and has no relevance to malignancy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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