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. 1984 Jan;49(1):11–15. doi: 10.1038/bjc.1984.3

An immunohistological study of testicular germ cell tumours using two different monoclonal antibodies against placental alkaline phosphatase.

A A Epenetos, P Travers, K C Gatter, R D Oliver, D Y Mason, W F Bodmer
PMCID: PMC1976671  PMID: 6362705

Abstract

Using two monoclonal antibodies directed against placental alkaline phosphatase (H17E2 and D20L) the immunohistological staining of testicular germ cell tumours was compared with that of a wide range of normal and malignant tissues. All seminomas and malignant teratomas tested gave strong positive labelling with H17E2 but were either negative or only patchily positive with D20L. Neither antibody gave any positive reaction on the normal tissues tested. All other malignancies were negative with both antibodies apart from two cases of ovarian and one case of endometrical cancer (strongly stained by H17E2) and three cases of colonic carcinoma (weakly and patchily stained by both H17E2 and D20L). This indicates that germ cell neoplasms generally express a form of placental alkaline phosphatase recognised by antibody H17E2.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Badger K. S., Sussman H. H. Structural evidence that human liver and placental alkaline phosphatase isoenzymes are coded by different genes. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2201–2205. doi: 10.1073/pnas.73.7.2201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berche C., Mach J. P., Lumbroso J. D., Langlais C., Aubry F., Buchegger F., Carrel S., Rougier P., Parmentier C., Tubiana M. Tomoscintigraphy for detecting gastrointestinal and medullary thyroid cancers: first clinical results using radiolabelled monoclonal antibodies against carcinoembryonic antigen. Br Med J (Clin Res Ed) 1982 Nov 20;285(6353):1447–1451. doi: 10.1136/bmj.285.6353.1447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chang C. H., Angellis D., Fishman W. H. Presence of the rare D-variant heat-stable, placental-type alkaline phosphatase in normal human testis. Cancer Res. 1980 May;40(5):1506–1510. [PubMed] [Google Scholar]
  4. Epenetos A. A., Britton K. E., Mather S., Shepherd J., Granowska M., Taylor-Papadimitriou J., Nimmon C. C., Durbin H., Hawkins L. R., Malpas J. S. Targeting of iodine-123-labelled tumour-associated monoclonal antibodies to ovarian, breast, and gastrointestinal tumours. Lancet. 1982 Nov 6;2(8306):999–1005. doi: 10.1016/s0140-6736(82)90046-0. [DOI] [PubMed] [Google Scholar]
  5. Farrands P. A., Perkins A. C., Pimm M. V., Embleton M. J., Hardy J. D., Baldwin R. W., Hardcastle J. D. Radioimmunodetection of human colorectal cancers by an anti-tumour monoclonal antibody. Lancet. 1982 Aug 21;2(8295):397–400. doi: 10.1016/s0140-6736(82)90437-8. [DOI] [PubMed] [Google Scholar]
  6. Fishman L., Miyayama H., Driscoll S. G., Fishman W. H. Developmental phase-specific alkaline phosphatase isoenzymes of human placenta and their occurrence in human cancer. Cancer Res. 1976 Jul;36(7 Pt 1):2268–2273. [PubMed] [Google Scholar]
  7. Goldstein D. J., Rogers C., Harris H. Evolution of alkaline phosphatases in primates. Proc Natl Acad Sci U S A. 1982 Feb;79(3):879–883. doi: 10.1073/pnas.79.3.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  9. Lange P. H., Millan J. L., Stigbrand T., Vessella R. L., Ruoslahti E., Fishman W. H. Placental alkaline phosphatase as a tumor marker for seminoma. Cancer Res. 1982 Aug;42(8):3244–3247. [PubMed] [Google Scholar]
  10. McLaughlin P. J., Cheng M. H., Slade M. B., Johnson P. M. Expression on cultured human tumour cells of placental trophoblast membrane antigens and placental alkaline phosphatase defined by monoclonal antibodies. Int J Cancer. 1982 Jul 15;30(1):21–26. doi: 10.1002/ijc.2910300105. [DOI] [PubMed] [Google Scholar]
  11. Millán J. L., Stigbrand T., Ruoslahti E., Fishman W. H. Characterization and use of an allotype-specific monoclonal antibody to placental alkaline phosphatase in the study of cancer-related phosphatase polymorphism. Cancer Res. 1982 Jun;42(6):2444–2449. [PubMed] [Google Scholar]
  12. Nathanson L., Fishman W. H. New observations on the Regan isoenzyme of alkaline phosphatase in cancer patients. Cancer. 1971 Jun;27(6):1388–1397. doi: 10.1002/1097-0142(197106)27:6<1388::aid-cncr2820270619>3.0.co;2-0. [DOI] [PubMed] [Google Scholar]
  13. Oi V. T., Jones P. P., Goding J. W., Herzenberg L. A., Herzenberg L. A. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens. Curr Top Microbiol Immunol. 1978;81:115–120. doi: 10.1007/978-3-642-67448-8_18. [DOI] [PubMed] [Google Scholar]
  14. Stolbach L. L., Krant M. J., Fishman W. H. Ectopic production of an alkaline phosphatase isoenzyme in patients with cancer. N Engl J Med. 1969 Oct 2;281(14):757–762. doi: 10.1056/NEJM196910022811403. [DOI] [PubMed] [Google Scholar]
  15. Stragand J. J., Bergerat J. P., White R. A., Hokanson J., Drewinko B. Biological and cell kinetic properties of a human colonic adenocarcinoma (LoVo) grown in athymic mice. Cancer Res. 1980 Aug;40(8 Pt 1):2846–2852. [PubMed] [Google Scholar]
  16. Uchida T., Shimoda T., Miyata H., Shikata T., Iino S., Suzuki H., Oda T., Hirano K., Sugiura M. Immunoperoxidase study of alkaline phosphatase in testicular tumor. Cancer. 1981 Sep 15;48(6):1455–1462. doi: 10.1002/1097-0142(19810915)48:6<1455::aid-cncr2820480630>3.0.co;2-x. [DOI] [PubMed] [Google Scholar]
  17. Wada H. G., Shindelman J. E., Ortmeyer A. E., Sussman H. H. Demonstration of placental alkaline phosphatase in human breast cancer. Int J Cancer. 1979 Jun 15;23(6):781–787. doi: 10.1002/ijc.2910230608. [DOI] [PubMed] [Google Scholar]

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