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. 1984 Feb;49(2):149–159. doi: 10.1038/bjc.1984.27

Characterisation of breast cancer infiltrates using monoclonal antibodies to human leucocyte antigens.

D J Rowe, P C Beverley
PMCID: PMC1976689  PMID: 6365132

Abstract

Serial frozen sections from eleven patients with malignant breast tumours and five patients with benign disease were studied by indirect immunoperoxidase using a panel of mouse monoclonal antibodies to human leucocyte antigens. More infiltrating leucocytes were seen in tumour sections than those of benign conditions. A considerable proportion of the infiltrating cells were T cells, and more of these were of the suppressor/cytotoxic subset than the helper/inducer subset. The T cells were apparently not all activated as indicated by lower levels of staining with anti HLA-DR than anti-leucocyte antibody. Diffuse staining was sometimes seen with HLA-DR and T cell subset antibodies. Tumour cells did not stain or were only very weakly positive with anti HLA-A, B, C.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beverley P. C., Callard R. E. Distinctive functional characteristics of human "T" lymphocytes defined by E rosetting or a monoclonal anti-T cell antibody. Eur J Immunol. 1981 Apr;11(4):329–334. doi: 10.1002/eji.1830110412. [DOI] [PubMed] [Google Scholar]
  2. Black M. M., Barclay T. H., Hankey B. F. Prognosis in breast cancer utilizing histologic characteristics of the primary tumor. Cancer. 1975 Dec;36(6):2048–2055. doi: 10.1002/cncr.2820360919. [DOI] [PubMed] [Google Scholar]
  3. Black M. M. Human breast cancer. A model for cancer immunology. Isr J Med Sci. 1973 Mar;9(3):284–299. [PubMed] [Google Scholar]
  4. Black M. M., Leis H. P., Jr, Shore B., Zachrau R. E. Cellular hypersensitivity to breast cancer. Assessment by a leukocyte migration procedure. Cancer. 1974 Apr;33(4):952–958. doi: 10.1002/1097-0142(197404)33:4<952::aid-cncr2820330409>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  5. Brodsky F. M., Parham P., Barnstable C. J., Crumpton M. J., Bodmer W. F. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979;47:3–61. doi: 10.1111/j.1600-065x.1979.tb00288.x. [DOI] [PubMed] [Google Scholar]
  6. Cutler S. J., Black M. M., Mork T., Harvei S., Freeman C. Further observations on prognostic factors in cancer of the female breast. Cancer. 1969 Oct;24(4):653–667. doi: 10.1002/1097-0142(196910)24:4<653::aid-cncr2820240402>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  7. Fleming K. A., McMichael A., Morton J. A., Woods J., McGee J. O. Distribution of HLA class 1 antigens in normal human tissue and in mammary cancer. J Clin Pathol. 1981 Jul;34(7):779–784. doi: 10.1136/jcp.34.7.779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fox R. I., Carstens S. A., Fong S., Robinson C. A., Howell F., Vaughan J. H. Use of monoclonal antibodies to analyze peripheral blood and salivary gland lymphocyte subsets in Sjögren's syndrome. Arthritis Rheum. 1982 Apr;25(4):419–426. doi: 10.1002/art.1780250410. [DOI] [PubMed] [Google Scholar]
  9. Heidenreich W., Jagla K., Schüssler J., Börner P., Dehnhard F., Kalden J. R., Leibold W., Peter H. H., Deicher H. Immunological characterization of mononuclear cells in peripheral blood and regional lymph nodes of breast cancer patients. Cancer. 1979 Apr;43(4):1308–1313. doi: 10.1002/1097-0142(197904)43:4<1308::aid-cncr2820430419>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  10. Humphrey L. J., Singla O., Volenec F. J. Immunologic responsiveness of the breast cancer patient. Cancer. 1980 Aug 15;46(4 Suppl):893–898. doi: 10.1002/1097-0142(19800815)46:4+<893::aid-cncr2820461307>3.0.co;2-v. [DOI] [PubMed] [Google Scholar]
  11. Keller S. E., Ioachim H. L., Pearse T., Siletti D. M. Decreased T-lymphocytes in patients with mammary cancer. Am J Clin Pathol. 1976 Apr;65(4):445–449. doi: 10.1093/ajcp/65.4.445. [DOI] [PubMed] [Google Scholar]
  12. LAMB D., PILNEY F., KELLY W. D., GOOD R. A. A comparative study of the incidence of anergy in patients with carcinoma, leukemia, hodgkin's disease and other lymphomas. J Immunol. 1962 Oct;89:555–558. [PubMed] [Google Scholar]
  13. Lauder I., Aherne W., Stewart J., Sainsbury R. Macrophage infiltration of breast tumours: a prospective study. J Clin Pathol. 1977 Jun;30(6):563–568. doi: 10.1136/jcp.30.6.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ledbetter J. A., Evans R. L., Lipinski M., Cunningham-Rundles C., Good R. A., Herzenberg L. A. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981 Feb 1;153(2):310–323. doi: 10.1084/jem.153.2.310. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McMichael A. HLA restriction of human cytotoxic T lymphocytes specific for influenza virus. Poor recognition of virus associated with HLA A2. J Exp Med. 1978 Dec 1;148(6):1458–1467. doi: 10.1084/jem.148.6.1458. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nemoto T., Han T., Minowada J., Angkur V., Chamberlain A., Dao L. Cell-mediated immune status of breast cancer patients: evaluation by skin tests, lymphocyte stimulation, and counts of rosette-forming cells. J Natl Cancer Inst. 1974 Sep;53(3):641–645. doi: 10.1093/jnci/53.3.641. [DOI] [PubMed] [Google Scholar]
  17. Rowe D., Isenberg D. A., Beverley P. C. Monoclonal antibodies to human leucocyte antigens in polymyositis and muscular dystrophy. Clin Exp Immunol. 1983 Nov;54(2):327–336. [PMC free article] [PubMed] [Google Scholar]
  18. Stein J. A., Adler A., Efraim S. B., Maor M. Immunocompetence, immunosuppression, and human breast cancer. I. An analysis of their relationship by known parameters of cell-mediated immunity in well-defined clinical stages of disease. Cancer. 1976 Sep;38(3):1171–1187. doi: 10.1002/1097-0142(197609)38:3<1171::aid-cncr2820380319>3.0.co;2-8. [DOI] [PubMed] [Google Scholar]
  19. Travers P. J., Arklie J. L., Trowsdale J., Patillo R. A., Bodmer W. F. Lack of expression of HLA-ABC antigens in choriocarcinoma and other human tumor cell lines. Natl Cancer Inst Monogr. 1982;60:175–180. [PubMed] [Google Scholar]

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