Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1984 Feb;49(2):173–179. doi: 10.1038/bjc.1984.29

Cellular DNA content--a stable feature in epithelial ovarian cancer.

M L Friedlander, I W Taylor, P Russell, M H Tattersall
PMCID: PMC1976707  PMID: 6696818

Abstract

Detailed flow cytometric analysis of cellular DNA content was performed on neoplastic tissue from 33 patients with malignant common epithelial ovarian tumours in order to investigate the intratumoral stability of ploidy and proliferative fraction. There was a remarkable stability, both spatial and temporal, in the DNA pattern for any particular tumour. Of 24 tumours that were analysed in multiple areas tumour ploidy was found to be a stable marker in all but 3 cases where regional variations were evident. In 9 patients serial analyses were performed on tumour obtained either at initial diagnosis (6 patients) or second look laparotomy (3 patients) and then some time later (7-17 months) at relapse or death and in all cases the tumour ploidy remained unchanged. In addition, 10 ovarian carcinomas established in nude mice have maintained a DNA content during serial passage similar to that of the original implanted tumour. In contrast in 50% of tumours that were evaluable for S-phase analysis we demonstrated a considerable intratumoral variability in the S-phase fraction. We conclude that cellular DNA content is a stable feature of ovarian carcinoma while S-phase fraction is commonly subject to intratumoral variation.

Full text

PDF
173

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkin N. B. Modal DNA value and chromosome number in ovarian neoplasia. A clinical and histopathologic assessment. Cancer. 1971 May;27(5):1064–1073. doi: 10.1002/1097-0142(197105)27:5<1064::aid-cncr2820270510>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  2. Barlogie B., Drewinko B., Schumann J., Göhde W., Dosik G., Latreille J., Johnston D. A., Freireich E. J. Cellular DNA content as a marker of neoplasia in man. Am J Med. 1980 Aug;69(2):195–203. doi: 10.1016/0002-9343(80)90379-4. [DOI] [PubMed] [Google Scholar]
  3. Barlogie B., Göhde W., Johnston D. A., Smallwood L., Schumann J., Drewinko B., Freireich E. J. Determination of ploidy and proliferative characteristics of human solid tumors by pulse cytophotometry. Cancer Res. 1978 Oct;38(10):3333–3339. [PubMed] [Google Scholar]
  4. Barlogie B., Hittelman W., Spitzer G., Trujilo J. M., Hart J. S., Smallwood L., Drewinko B. Correlation of DNA distribution abnormalities with cytogenetic findings in human adult leukemia and lymphoma. Cancer Res. 1977 Dec;37(12):4400–4407. [PubMed] [Google Scholar]
  5. Bunn P. A., Jr, Krasnow S., Makuch R. W., Schlam M. L., Schechter G. P. Flow cytometric analysis of DNA content of bone marrow cells in patients with plasma cell myeloma: clinical implications. Blood. 1982 Mar;59(3):528–535. [PubMed] [Google Scholar]
  6. Costa A., Mazzini G., Del Bino G., Silvestrini R. DNA content and kinetic characteristics of non-Hodgkin's lymphoma: determined by flow cytometry and autoradiography. Cytometry. 1981 Nov;2(3):185–188. doi: 10.1002/cyto.990020310. [DOI] [PubMed] [Google Scholar]
  7. Dosik G. M., Barlogie B., Göhde W., Johnston D., Tekell J. L., Drewinko B. Flow cytometry of DNA content in human bone marrow: a critical reappraisal. Blood. 1980 May;55(5):734–740. [PubMed] [Google Scholar]
  8. Friedlander M. L., Taylor I. W., Russell P., Musgrove E. A., Hedley D. H., Tattersall M. H. Ploidy as a prognostic factor in ovarian cancer. Int J Gynecol Pathol. 1983;2(1):55–63. doi: 10.1097/00004347-198301000-00005. [DOI] [PubMed] [Google Scholar]
  9. Kraemer P. M., Petersen D. F., Van Dilla M. A. DNA constancy in heteroploidy and the stem line theory of tumors. Science. 1971 Nov 12;174(4010):714–717. doi: 10.1126/science.174.4010.714. [DOI] [PubMed] [Google Scholar]
  10. Mackillop W. J., Trent J. M., Stewart S. S., Buick R. N. Tumor progression studied by analysis of cellular features of serial ascitic ovarian carcinoma tumors. Cancer Res. 1983 Feb;43(2):874–878. [PubMed] [Google Scholar]
  11. Milthorpe B. FMFPAK1: a program package for routine analysis of single parameter flow microfluorimetric data on a low cost mini-computer. Comput Biomed Res. 1980 Oct;13(5):417–429. doi: 10.1016/0010-4809(80)90039-7. [DOI] [PubMed] [Google Scholar]
  12. Nervi C., Badaracco G., Maisto A., Mauro F., Tirindelli-Danesi D., Starace G. Cytometric evidence of cytogenetic and proliferative heterogeneity of human solid tumors. Cytometry. 1982 Mar;2(5):303–308. doi: 10.1002/cyto.990020507. [DOI] [PubMed] [Google Scholar]
  13. Perez D. J., Taylor I. W., Milthorpe B. K., McGovern V. J., Tattersall M. H. Identification and quantitation of tumour cells in cell suspensions: a comparison of cytology and flow cytometry. Br J Cancer. 1981 Apr;43(4):526–531. doi: 10.1038/bjc.1981.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Taylor I. W. A rapid single step staining technique for DNA analysis by flow microfluorimetry. J Histochem Cytochem. 1980 Sep;28(9):1021–1024. doi: 10.1177/28.9.6157714. [DOI] [PubMed] [Google Scholar]
  15. Taylor I. W., Milthorpe B. K. An evaluation of DNA fluorochromes, staining techniques, and analysis for flow cytometry. I. Unperturbed cell populations. J Histochem Cytochem. 1980 Nov;28(11):1224–1232. doi: 10.1177/28.11.6159392. [DOI] [PubMed] [Google Scholar]
  16. Taylor I. W., Musgrove E. A., Friedlander M. L., Foo M. S., Hedley D. W. The influence of age on the DNA ploidy levels of breast tumours. Eur J Cancer Clin Oncol. 1983 May;19(5):623–628. doi: 10.1016/0277-5379(83)90178-5. [DOI] [PubMed] [Google Scholar]
  17. Vindeløv L. L., Hansen H. H., Christensen I. J., Spang-Thomsen M., Hirsch F. R., Hansen M., Nissen N. I. Clonal heterogeneity of small-cell anaplastic carcinoma of the lung demonstrated by flow-cytometric DNA analysis. Cancer Res. 1980 Nov;40(11):4295–4300. [PubMed] [Google Scholar]
  18. Wolley R. C., Schreiber K., Koss L. G., Karas M., Sherman A. DNA distribution in human colon carcinomas and its relationship to clinical behavior. J Natl Cancer Inst. 1982 Jul;69(1):15–22. [PubMed] [Google Scholar]
  19. van Haaften-Day C., Russell P., Rugg C., Wills E. J., Tattersall M. H. Flow cytometric and morphological studies of ovarian carcinoma cell lines and xenografts. Cancer Res. 1983 Aug;43(8):3725–3731. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES