Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1984 Feb;49(2):193–198. doi: 10.1038/bjc.1984.31

An investigation of cellular components released from human renal cancer and foetal kidney xenografts in nude mice (nu/nu) by cross-immunization of hairy littermate relatives.

P N Matthews, J Hermon-Taylor, A G Grant
PMCID: PMC1976710  PMID: 6696819

Abstract

The release of components from human kidney tumour xenografts (GYL) and human foetal kidney explants maintained in nude mice has been studied. The GYL tumour released antigens into the serum which could be detected by the generation of antibodies following cross-immunisation of closely related hairy litter mate (HLM) mice. The production of anti-GYL antibody was monitored by an I125 binding assay using viable GYL tumour cells. In 2/16 hairy litter mate mice, cell surface antibody binding by GYL cells was twice that found with 8 other human tumour cell lines (including 2 other kidney cancer cell lines). Absorption of these antisera with 10(7) GYL tumour cells completely abolished this response, where 50%, 38% and 25% of activity remained following absorption with; a normal kidney cell line, a homogenate of normal kidney and a mixed pool of human tumour cells. Six out of 8 GYL tumour bearing nude mice tested had elevated plasma levels of HCG. Absorption of the HLM antisera with an excess of commercial HCG abrogated I125 binding by only 15%, suggesting that antibody production was not directed primarily against ectopic HCG.

Full text

PDF
193

Images in this article

196Figure 4
on p.196

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ackermann R. Tumour-associated antibodies against renal cell carcinomas detected by immunofluorescence. Eur Urol. 1975;1(3):154–156. [PubMed] [Google Scholar]
  2. Bastert G., Althoff P., Usadel K. H., Fortmeyer H. P., Schmidt-Matthiesen H. Heterotransplantation of human fetal pituitaries in nude mice. Endocrinology. 1977 Aug;101(2):365–368. doi: 10.1210/endo-101-2-365. [DOI] [PubMed] [Google Scholar]
  3. Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 13-1972. N Engl J Med. 1972 Mar 30;286(13):713–719. doi: 10.1056/NEJM197203302861308. [DOI] [PubMed] [Google Scholar]
  4. Chisholm G. D. Nephrogenic ridge tumors and their syndromes. Ann N Y Acad Sci. 1974;230:403–423. doi: 10.1111/j.1749-6632.1974.tb14474.x. [DOI] [PubMed] [Google Scholar]
  5. Davies G., Duke D., Grant A. G., Kelly S. A., Hermon-Taylor J. Growth of human digestive-tumour xenografts in athymic nude rats. Br J Cancer. 1981 Jan;43(1):53–58. doi: 10.1038/bjc.1981.7. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dekernion J. B., Ramming K. P., Gupta R. K. The detection and clinical significance of antibodies to tumor-associated antigens in patients with renal cell carcinoma. J Urol. 1979 Sep;122(3):300–305. doi: 10.1016/s0022-5347(17)56376-4. [DOI] [PubMed] [Google Scholar]
  7. DiPersio L., Dingle S., Michael J. G., Pesce A. J. Release of beta 2-microglobulin by human tumors grown in nude mice. Exp Cell Biol. 1980;48(6):429–438. doi: 10.1159/000163009. [DOI] [PubMed] [Google Scholar]
  8. Freed S. Z., Halperin J. P., Gordon M. Idiopathic regression of metastases from renal cell carcinoma. J Urol. 1977 Oct;118(4):538–542. doi: 10.1016/s0022-5347(17)58099-4. [DOI] [PubMed] [Google Scholar]
  9. Grant A. G., Duke D., Hermon-Taylor J. Establishment and characterization of primary human pancreatic carcinoma in continuous cell culture and in nude mice. Br J Cancer. 1979 Feb;39(2):143–151. doi: 10.1038/bjc.1979.24. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Grant A. G., Duke D. Production of antibodies against antigens released from human pancreatic tumour xenografts. Br J Cancer. 1981 Sep;44(3):388–395. doi: 10.1038/bjc.1981.196. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hellström I. E., Hellström K. E., Pierce G. E., Bill A. H. Demonstration of cell-bound and humoral immunity against neuroblastoma cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1231–1238. doi: 10.1073/pnas.60.4.1231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Herberman R. B. Immunogenicity of tumor antigens. Biochim Biophys Acta. 1977 Dec 23;473(2):93–119. doi: 10.1016/0304-419x(77)90002-6. [DOI] [PubMed] [Google Scholar]
  13. Kameya T., Shimosato Y., Tumuraya M., Ohsawa N., Nomura T. Human gastric choriocarcinoma serially transplanted in nude mice. J Natl Cancer Inst. 1976 Feb;56(2):325–332. doi: 10.1093/jnci/56.2.325. [DOI] [PubMed] [Google Scholar]
  14. Katsuoka Y., Baba S., Hata M., Tazaki H. Transplantation of human renal cell carcinoma to the nude mice: as an intermediate of in vivo and in vitro studies. J Urol. 1976 Apr;115(4):373–376. doi: 10.1016/s0022-5347(17)59208-3. [DOI] [PubMed] [Google Scholar]
  15. Matthews P. N., Grant A. G., Hermon-Taylor J. The growth of human bladder and kidney cancers as xenografts in nude mice and rats. Urol Res. 1982;10(6):293–299. doi: 10.1007/BF00255876. [DOI] [PubMed] [Google Scholar]
  16. Povlsen C. O., Skakkebaek N. E., Rygaard J., Jensen G. Heterotransplantation of human foetal organs to the mouse mutant nude. Nature. 1974 Mar 15;248(445):247–249. doi: 10.1038/248247a0. [DOI] [PubMed] [Google Scholar]
  17. Rigby C. C., Franks L. M. A human tissue culture cell line from a transitional cell tumour of the urinary bladder: growth, chromosone pattern and ultrastructure. Br J Cancer. 1970 Dec;24(4):746–754. doi: 10.1038/bjc.1970.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sprent J., Miller J. F. Thoracic duct lymphocytes from nude mice: migratory properties and life-span. Eur J Immunol. 1972 Aug;2(4):384–387. doi: 10.1002/eji.1830020420. [DOI] [PubMed] [Google Scholar]
  19. Sufrin G., Mirand E. A., Moore R. H., Chu T. M., Murphy G. P. Hormones in renal cancer. J Urol. 1977 Apr;117(4):433–438. doi: 10.1016/s0022-5347(17)58490-6. [DOI] [PubMed] [Google Scholar]
  20. Ueda R., Ogata S., Morrissey D. M., Finstad C. L., Szkudlarek J., Whitmore W. F., Oettgen H. F., Lloyd K. O., Old L. J. Cell surface antigens of human renal cancer defined by mouse monoclonal antibodies: identification of tissue-specific kidney glycoproteins. Proc Natl Acad Sci U S A. 1981 Aug;78(8):5122–5126. doi: 10.1073/pnas.78.8.5122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Uriel J. Retrodifferentiation and the fetal patterns of gene expression in cancer. Adv Cancer Res. 1979;29:127–174. doi: 10.1016/s0065-230x(08)60847-7. [DOI] [PubMed] [Google Scholar]
  22. Usadel K. H., Bastert G., Schwedes U., Obert I., Fortmeyer H. P., Schöffling K. Human fetal pancreas transplants in nu/nu mice. Lancet. 1977 Feb 12;1(8007):365–365. doi: 10.1016/s0140-6736(77)91166-7. [DOI] [PubMed] [Google Scholar]
  23. Woo J., Singer F. R. Radioimmunoassay for human parathyroid hormone. Clin Chim Acta. 1974 Jul 31;54(2):161–168. doi: 10.1016/0009-8981(74)90233-2. [DOI] [PubMed] [Google Scholar]
  24. Young R. K., Cailleau R. M., Mackay B., Reeves W. J. Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma. In Vitro. 1974 Jan-Feb;9(4):239–245. doi: 10.1007/BF02616069. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES