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British Journal of Cancer logoLink to British Journal of Cancer
. 1984 Apr;49(4):453–458. doi: 10.1038/bjc.1984.72

The effect of fibre size on the in vivo activity of UICC crocidolite.

J C Wagner, D M Griffiths, R J Hill
PMCID: PMC1976765  PMID: 6324841

Abstract

Standard (UICC) crocidolite was subjected to ball milling to reduce the length of the fibre. These milled materials and the original standard sample were injected into the pleural cavity of rats to determine their ability to induce mesothelioma. Previous in vitro work on the same materials had suggested that biological activity was related to fibres greater than 6.5 microns in length and that the material milled for 4 and 8 h did not contain fibres in this range and was biologically inactive. The results of the animal work, however, did not follow this pattern; mesotheliomas occurred in rats in all treatment groups including the 4 and 8 h milled samples. Examination of the tissues and the dust recovered from them showed the presence of fibres greater than the suggested threshold. Attention is drawn to the problems associated with drawing conclusions from size distributions and in vitro studies without considering in vivo mechanisms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashcroft T., Heppleston A. G. The optical and electron microscopic determination of pulmonary asbestos fibre concentration and its relation to the human pathological reaction. J Clin Pathol. 1973 Mar;26(3):224–234. doi: 10.1136/jcp.26.3.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brown R. C., Chamberlain M., Griffiths D. M., Timbrell V. The effect of fibre size on the in vitro biological activity of three types of amphibole asbestos. Int J Cancer. 1978 Dec;22(6):721–727. doi: 10.1002/ijc.2910220614. [DOI] [PubMed] [Google Scholar]
  3. Griffiths D. M., Hill R. J. The effects of dispersion on fibrous clays in vitro and in vivo. Ann Occup Hyg. 1983;27(4):405–414. doi: 10.1093/annhyg/27.4.405. [DOI] [PubMed] [Google Scholar]
  4. Gylseth B., Baunan R. H., Bruun R. Analysis of inorganic fiber concentrations in biological samples by scanning electron microscopy. Scand J Work Environ Health. 1981 Jun;7(2):101–108. doi: 10.5271/sjweh.2562. [DOI] [PubMed] [Google Scholar]
  5. Kolev K. Experimentally induced mesothelioma in white rats in response to intraperitoneal administration of amorphous crocidolite asbestos: preliminary report. Environ Res. 1982 Oct;29(1):123–133. doi: 10.1016/0013-9351(82)90013-5. [DOI] [PubMed] [Google Scholar]
  6. Stanton M. F., Laynard M., Tegeris A., Miller E., May M., Kent E. Carcinogenicity of fibrous glass: pleural response in the rat in relation to fiber dimension. J Natl Cancer Inst. 1977 Mar;58(3):587–603. doi: 10.1093/jnci/58.3.587. [DOI] [PubMed] [Google Scholar]
  7. WAGNER J. C., SLEGGS C. A., MARCHAND P. Diffuse pleural mesothelioma and asbestos exposure in the North Western Cape Province. Br J Ind Med. 1960 Oct;17:260–271. doi: 10.1136/oem.17.4.260. [DOI] [PMC free article] [PubMed] [Google Scholar]

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