Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1984 Dec;50(6):809–814. doi: 10.1038/bjc.1984.260

A comparison of the expression of the DIDS-binding proteins from normal and tumorigenic human cells.

M R Banyard
PMCID: PMC1977008  PMID: 6498078

Abstract

A monoclonal antibody was prepared against DIDS, an inhibitor of anion transport, and used to compare the occurrence and distribution of DIDS-binding sites of tumorigenic and non-tumorigenic human somatic-cell hybrids. The monoclonal antibody (E8) was produced by the fusion of the mouse myeloma (NS-1) with mouse spleen cells and is of the IgG1 subclass. The apparent half-saturation of DIDS for HEp-2 cells is 16 microM and the reaction is rapid. The number of binding sites on tumorigenic and non-tumorigenic hybrid cells was the same. The DIDS-binding protein occurs homogeneously on all cells, a characteristic which distinguishes it from the possible tumour antigen recognised by the M/27 monoclonal antibody.

Full text

PDF
809

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banyard M. R., White M. K. Association of an integral membrane protein with glucose transport and with anion transport. J Cell Sci. 1984 Apr;67:45–62. doi: 10.1242/jcs.67.1.45. [DOI] [PubMed] [Google Scholar]
  2. Cabantchik Z. I., Rothstein A. Membrane proteins related to anion permeability of human red blood cells. I. Localization of disulfonic stilbene binding sites in proteins involved in permeation. J Membr Biol. 1974;15(3):207–226. doi: 10.1007/BF01870088. [DOI] [PubMed] [Google Scholar]
  3. Chen T. R. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res. 1977 Feb;104(2):255–262. doi: 10.1016/0014-4827(77)90089-1. [DOI] [PubMed] [Google Scholar]
  4. Deuticke B., Beyer E., Forst B. Discrimination of three parallel pathways of lactate transport in the human erythrocyte membrane by inhibitors and kinetic properties. Biochim Biophys Acta. 1982 Jan 4;684(1):96–110. doi: 10.1016/0005-2736(82)90053-0. [DOI] [PubMed] [Google Scholar]
  5. Gingrich R. D., Wouters M., Bramwell M. E., Harris H. Immunological delineation in normal and malignant cells of a membrane protein involved in glucose transport. II. Function of the antigen. J Cell Sci. 1981 Dec;52:121–135. doi: 10.1242/jcs.52.1.121. [DOI] [PubMed] [Google Scholar]
  6. Jennings M. L., Adams-Lackey M. A rabbit erythrocyte membrane protein associated with L-lactate transport. J Biol Chem. 1982 Nov 10;257(21):12866–12871. [PubMed] [Google Scholar]
  7. Johnson J. H., Belt J. A., Dubinsky W. P., Zimniak A., Racker E. Inhibition of lactate transport in Ehrlich ascites tumor cells and human erythrocytes by a synthetic anhydride of lactic acid. Biochemistry. 1980 Aug 5;19(16):3836–3840. doi: 10.1021/bi00557a029. [DOI] [PubMed] [Google Scholar]
  8. L'Allemain G., Franchi A., Cragoe E., Jr, Pouysségur J. Blockade of the Na+/H+ antiport abolishes growth factor-induced DNA synthesis in fibroblasts. Structure-activity relationships in the amiloride series. J Biol Chem. 1984 Apr 10;259(7):4313–4319. [PubMed] [Google Scholar]
  9. Lepke S., Fasold H., Pring M., Passow H. A study of the relationship between inhibition of anion exchange and binding to the red blood cell membrane of 4,4'-diisothiocyano stilbene-2,2'-disulfonic acid (DIDS) and its dihydro derivative (H2DIDS). J Membr Biol. 1976 Oct 20;29(1-2):147–177. doi: 10.1007/BF01868957. [DOI] [PubMed] [Google Scholar]
  10. Letarte-Muirhead M., Barclay A. N., Williams A. F. Purification of the Thy-1 molecule, a major cell-surface glycoprotein of rat thymocytes. Biochem J. 1975 Dec;151(3):685–697. doi: 10.1042/bj1510685. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roos A., Boron W. F. Intracellular pH. Physiol Rev. 1981 Apr;61(2):296–434. doi: 10.1152/physrev.1981.61.2.296. [DOI] [PubMed] [Google Scholar]
  12. Spencer T. L., Lehninger A. L. L-lactate transport in Ehrlich ascites-tumour cells. Biochem J. 1976 Feb 15;154(2):405–414. doi: 10.1042/bj1540405. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stanbridge E. J., Der C. J., Doersen C. J., Nishimi R. Y., Peehl D. M., Weissman B. E., Wilkinson J. E. Human cell hybrids: analysis of transformation and tumorigenicity. Science. 1982 Jan 15;215(4530):252–259. doi: 10.1126/science.7053574. [DOI] [PubMed] [Google Scholar]
  14. Vaupel P. W., Frinak S., Bicher H. I. Heterogeneous oxygen partial pressure and pH distribution in C3H mouse mammary adenocarcinoma. Cancer Res. 1981 May;41(5):2008–2013. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES