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. 1985 Jun;51(6):865–875. doi: 10.1038/bjc.1985.132

Lung tumour cell lines synthesizing peptide hormones established from tumours of four histological types: characterization of the cell lines and analysis of their peptide hormone production.

W Luster, C Gropp, H F Kern, K Havemann
PMCID: PMC1977074  PMID: 2988594

Abstract

Thirty permanent and more than 60 primary tumour cell lines were established from pleural and pericardial exudates or wedge biopsies from human bronchial carcinoma. The permanent cell lines have their origin in 6 small cell, 5 large cell, 9 squamous and 5 adeno carcinomas of the lung. Tumour cells were purified from non tumour cells by direct cloning in fluid cultures or by soft agar cloning. In vitro secretion of ACTH, bombesin, calcitonin, and neurotensin was demonstrated for lung tumour cells belonging to the four major histological types. Cell suspensions of peptide hormone secreting permanent cell cultures were grown to solid tumours after xenotransplantation into nude mice. Comparative ultrastructural examination of the primary tumour and of cells grown in tissue culture and in xenografts demonstrated the preservation of most tumour type specific structural criteria in the ex vivo/in vitro systems. The present data show that not only tumour cells from small cell carcinoma but also from other histological types are capable of synthesizing a broad spectrum of immunoreactive peptide hormones. This result might be interpreted as indicating a common expression of hormone biosynthesis and secretion by all lung tumours.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berson S. A., Yalow R. S. Parathyroid hormone in plasma in adenomatous hyperparathyroidism, uremia, and bronchogenic carcinoma. Science. 1966 Nov 18;154(3751):907–909. doi: 10.1126/science.154.3751.907. [DOI] [PubMed] [Google Scholar]
  2. Gazdar A. F., Carney D. N., Russell E. K., Sims H. L., Baylin S. B., Bunn P. A., Jr, Guccion J. G., Minna J. D. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 1980 Oct;40(10):3502–3507. [PubMed] [Google Scholar]
  3. Gropp C., Havemann K., Scheuer A. Ectopic hormones in lung cancer patients at diagnosis and during therapy. Cancer. 1980 Jul 15;46(2):347–354. doi: 10.1002/1097-0142(19800715)46:2<347::aid-cncr2820460223>3.0.co;2-s. [DOI] [PubMed] [Google Scholar]
  4. Horai R., Nishihara H., Tateishi R., Matsuda M., Hattori S. Oat-cell carcinoma of the lung simultaneously producing ACTH and serotonin. J Clin Endocrinol Metab. 1973 Aug;37(2):212–219. doi: 10.1210/jcem-37-2-212. [DOI] [PubMed] [Google Scholar]
  5. Luster W., Gropp C., Sostmann H., Kalbfleisch H., Havemann K. Demonstration of immunoreactive calcitonin in sera and tissues of lung cancer patients. Eur J Cancer Clin Oncol. 1982 Dec;18(12):1275–1283. doi: 10.1016/0277-5379(82)90129-8. [DOI] [PubMed] [Google Scholar]
  6. McIntosh C., Arnold R., Bothe E., Becker H., Köbberling J., Creutzfeldt W. Gastrointestinal somatostatin: extraction and radioimmunoassay in different species. Gut. 1978 Jul;19(7):655–663. doi: 10.1136/gut.19.7.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moody T. W., Pert C. B., Gazdar A. F., Carney D. N., Minna J. D. High levels of intracellular bombesin characterize human small-cell lung carcinoma. Science. 1981 Dec 11;214(4526):1246–1248. doi: 10.1126/science.6272398. [DOI] [PubMed] [Google Scholar]
  8. Pettengill O. S., Curphey T. J., Cate C. C., Flint C. F., Maurer L. H., Sorenson G. D. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp Cell Biol. 1980;48(4):279–297. doi: 10.1159/000162994. [DOI] [PubMed] [Google Scholar]
  9. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roos B. A., Lindall A. W., Baylin S. B., O'Neil J. A., Frelinger A. L., Birnbaum R. S., Lambert P. W. Plasma immunoreactive calcitonin in lung cancer. J Clin Endocrinol Metab. 1980 Apr;50(4):659–666. doi: 10.1210/jcem-50-4-659. [DOI] [PubMed] [Google Scholar]
  11. Roth J., LeRoith D., Shiloach J., Rosenzweig J. L., Lesniak M. A., Havrankova J. The evolutionary origins of hormones, neurotransmitters, and other extracellular chemical messengers: implications for mammalian biology. N Engl J Med. 1982 Mar 4;306(9):523–527. doi: 10.1056/NEJM198203043060907. [DOI] [PubMed] [Google Scholar]
  12. Sherwin S. A., Minna J. D., Gazdar A. F., Todaro G. J. Expression of epidermal and nerve growth factor receptors and soft agar growth factor production by human lung cancer cells. Cancer Res. 1981 Sep;41(9 Pt 1):3538–3542. [PubMed] [Google Scholar]
  13. Silva O. L., Becker K. L., Primack A., Doppman J., Snider R. H. Ectopic secretion of calcitonin by oat-cell carcinoma. N Engl J Med. 1974 May 16;290(20):1122–1124. doi: 10.1056/NEJM197405162902007. [DOI] [PubMed] [Google Scholar]
  14. Silva O. L., Broder L. E., Doppman J. L., Snider R. H., Moore C. F., Cohen M. H., Becker K. L. Calcitonin as a marker for bronchogenic cancer: a prospective study. Cancer. 1979 Aug;44(2):680–684. doi: 10.1002/1097-0142(197908)44:2<680::aid-cncr2820440240>3.0.co;2-j. [DOI] [PubMed] [Google Scholar]
  15. Skrabanek P., Powell D. Unifying concept of non-pituitary ACTH-secreting tumors: evidence of common origin of neural-crest tumors, carcinoids, and oat-cell carcinomas. Cancer. 1978 Sep;42(3):1263–1269. doi: 10.1002/1097-0142(197809)42:3<1263::aid-cncr2820420335>3.0.co;2-o. [DOI] [PubMed] [Google Scholar]
  16. Sorenson G. D., Pettengill O. S., Brinck-Johnsen T., Cate C. C., Maurer L. H. Hormone production by cultures of small-cell carcinoma of the lung. Cancer. 1981 Mar 15;47(6):1289–1296. doi: 10.1002/1097-0142(19810315)47:6<1289::aid-cncr2820470610>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  17. Wolfsen A. R., Odell W. D. ProACTH: use for early detection of lung cancer. Am J Med. 1979 May;66(5):765–772. doi: 10.1016/0002-9343(79)91114-8. [DOI] [PubMed] [Google Scholar]

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