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. 1985 Jun;51(6):767–774. doi: 10.1038/bjc.1985.120

Osteoclasts and the resorption of bone by transplanted mammary carcinoma in rats.

R L O'Grady, D A Cameron
PMCID: PMC1977083  PMID: 4005137

Abstract

Rat mammary tumour cells were grafted to parietal bones as an experimental model to study the nature of bone resorption around metastatic carcinomas in the skeleton. After periods of growth of from 6 to 56 days bones and tumours were removed and embedded in epoxy resin. The appearances were compared with those found when whole parathyroid glands were grafted in similar positions. Tumours were evident in all animals at the time of death and some were palpable five days after grafting. In 15 of the 21 animals with tumour, osteoclasts and resorption were found, and in only two of these were the tumour cells not separated from the bone surfaces. In 6 animals killed between 6 and 12 days after grafting there was new bone formation without resorption. There were osteoclasts and resorption under the grafted parathyroid glands which were always separated from the resorbing cells by fibrous tissue. The appearances of the bone surfaces under the tumours and the parathyroid glands suggested that the resorption in both situations was similar, was brought about by the secretion of a locally active agent and mediated by osteoclasts. This is further support for the role of osteoclasts in bone resorption around metastatic carcinomas.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnicot N. A. The local action of the parathyroid and other tissues on bone in intracerebral grafts. J Anat. 1948 Oct;82(Pt 4):233–248.2. [PMC free article] [PubMed] [Google Scholar]
  2. CHANG H. Y. Grafts of parathyroid and other tissues to bone. Anat Rec. 1951 Sep;111(1):23–47. doi: 10.1002/ar.1091110103. [DOI] [PubMed] [Google Scholar]
  3. Carter R. L., Pittam M. R. Squamous carcinomas of the head and neck: some patterns of spread. J R Soc Med. 1980 Jun;73(6):420–427. doi: 10.1177/014107688007300606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Carter R. L., Tsao S. W., Burman J. F., Pittam M. R., Clifford P., Shaw H. J. Patterns and mechanisms of bone invasion by squamous carcinomas of the head and neck. Am J Surg. 1983 Oct;146(4):451–455. doi: 10.1016/0002-9610(83)90229-5. [DOI] [PubMed] [Google Scholar]
  5. Dominguez J. H., Mundy G. R. Monocytes mediate osteoclastic bone resorption by prostaglandin production. Calcif Tissue Int. 1980;31(1):29–34. doi: 10.1007/BF02407164. [DOI] [PubMed] [Google Scholar]
  6. Eilon G., Mundy G. R. Direct resorption of bone by human breast cancer cells in vitro. Nature. 1978 Dec 14;276(5689):726–728. doi: 10.1038/276726a0. [DOI] [PubMed] [Google Scholar]
  7. Faccini J. M. The mode of growth of experimental metastases in rabbit femora. Virchows Arch A Pathol Anat Histol. 1974;364(3):249–263. doi: 10.1007/BF00433077. [DOI] [PubMed] [Google Scholar]
  8. Galasko C. S. Mechanisms of bone destruction in the development of skeletal metastases. Nature. 1976 Oct 7;263(5577):507–508. doi: 10.1038/263507a0. [DOI] [PubMed] [Google Scholar]
  9. Galasko C. S., Rawlins R., Bennett A. Timing of indomethacin in the control of prostaglandins, osteoclasts and bone destruction produced by VX2 carcinoma in rabbits. Br J Cancer. 1979 Sep;40(3):360–364. doi: 10.1038/bjc.1979.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Göthlin G., Ericsson J. L. On the histogenesis of the cells in fracture callus. Electron microscopic autoradiographic observations in parabiotic rats and studies on labeled monocytes. Virchows Arch B Cell Pathol. 1973 Mar 30;12(4):318–329. [PubMed] [Google Scholar]
  11. Hulth A., Olerud S. The reaction of bone to experimental cancer. Acta Orthop Scand. 1965;36(3):230–240. doi: 10.3109/17453676508989388. [DOI] [PubMed] [Google Scholar]
  12. MILCH R. A., CHANGUS G. W. Response of bone to tumor invasion. Cancer. 1956 Mar-Apr;9(2):340–351. doi: 10.1002/1097-0142(195603/04)9:2<340::aid-cncr2820090221>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  13. Mundy G. R., Raisz L. G., Cooper R. A., Schechter G. P., Salmon S. E. Evidence for the secretion of an osteoclast stimulating factor in myeloma. N Engl J Med. 1974 Nov 14;291(20):1041–1046. doi: 10.1056/NEJM197411142912001. [DOI] [PubMed] [Google Scholar]
  14. O'Grady R. L., Harrop P. J., Cameron D. A. Collagenolytic activity by malignant tumours. Pathology. 1982 Apr;14(2):135–138. doi: 10.3109/00313028209061286. [DOI] [PubMed] [Google Scholar]
  15. O'Grady R. L., Upfold L. I., Stephens R. W. Rat mammary carcinoma cells secrete active collagenase and activate latent enzyme in the stroma via plasminogen activator. Int J Cancer. 1981 Oct 15;28(4):509–515. doi: 10.1002/ijc.2910280418. [DOI] [PubMed] [Google Scholar]
  16. Pollard A. W., Feik S. A., Storey E. Remodelling of bone and bones: effects of translation and strain on transplants. Br J Exp Pathol. 1984 Dec;65(6):655–670. [PMC free article] [PubMed] [Google Scholar]
  17. Raisz L. G., Sandberg A. L., Goodson J. M., Simmons H. A., Mergenhagen S. E. Complement-dependent stimulation of prostaglandin synthesis and bone resorption. Science. 1974 Aug 30;185(4153):789–791. doi: 10.1126/science.185.4153.789. [DOI] [PubMed] [Google Scholar]
  18. Rodan G. A., Martin T. J. Role of osteoblasts in hormonal control of bone resorption--a hypothesis. Calcif Tissue Int. 1981;33(4):349–351. doi: 10.1007/BF02409454. [DOI] [PubMed] [Google Scholar]
  19. SHIVAS A. A., BLACK J. W., FINLAYSON N. D. THE GROWTH OF BROWN-PEARCE CARCINOMA IN THE MEDULLARY CAVITY OF THE RABBIT FEMUR. Br J Cancer. 1963 Dec;17:711–714. doi: 10.1038/bjc.1963.90. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Seyberth H. W. Prostaglandin-mediated hypercalcemia: a paraneoplastic syndrome. Klin Wochenschr. 1978 Apr 15;56(8):373–387. doi: 10.1007/BF01477292. [DOI] [PubMed] [Google Scholar]
  21. Silve C. M., Hradek G. T., Jones A. L., Arnaud C. D. Parathyroid hormone receptor in intact embryonic chicken bone: characterization and cellular localization. J Cell Biol. 1982 Aug;94(2):379–386. doi: 10.1083/jcb.94.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Storey E., Feik S. A. Remodelling of bone and bones. Effects of altered mechanical stress on anlages. Br J Exp Pathol. 1982 Apr;63(2):184–193. [PMC free article] [PubMed] [Google Scholar]
  23. Tashjian A. H., Jr Mechanisms of bone remodeling induced by tumors: tumor humors. Schweiz Med Wochenschr. 1981 Dec 5;111(49):1869–1872. [PubMed] [Google Scholar]
  24. Tsao S. W., Burman J. F., Easty D. M., Easty G. C., Carter R. L. Some mechanisms of local bone destruction by squamous carcinomas of the head and neck. Br J Cancer. 1981 Mar;43(3):392–401. doi: 10.1038/bjc.1981.60. [DOI] [PMC free article] [PubMed] [Google Scholar]

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