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. 1985 Aug;52(2):215–222. doi: 10.1038/bjc.1985.180

Colony-stimulating activity from the new metastatic TS/A cell line and its high- and low-metastatic clonal derivatives.

G Nicoletti, P Brambilla, C De Giovanni, P L Lollini, B Del Re, A Marocchi, P Mocarelli, G Prodi, P Nanni
PMCID: PMC1977095  PMID: 3875361

Abstract

We investigated the presence of colony-stimulating factor (CSF) in supernatants obtained from TS/A, a new metastatic murine cell line, and from its high-and low-metastatic clonal derivatives (E and F clones, respectively). TS/A cells produced a CSF in vitro that induced proliferation and differentiation of murine monocytic and granulocytic progenitors in agar cultures. In TS/A-bearing mice remarkable splenomegaly, blood granulocytosis and thymus depletion were observed along with a stimulatory activity in serum and a strong proliferative activity both in spleen and in bone marrow populations. Conditioned media from E clones showed an in vitro colony-stimulating activity greater than those of F clones. Mice injected subcutaneously with cells of all clones studied showed granulocytosis, splenomegaly and thymus depletion, although to varying degrees. However, no direct correlation between granulocytosis-splenomegaly and the number of spontaneous lung metastases was observed.

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Selected References

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  1. Asano S., Urabe A., Okabe T., Sato N., Kondo Y. Demonstration of granulopoietic factor(s) in the plasma of nude mice transplanted with a human lung cancer and in the tumor tissue. Blood. 1977 May;49(5):845–852. [PubMed] [Google Scholar]
  2. Balducci L., Hardy C. High proliferation of granulocyte-macrophage progenitors in tumor-bearing mice. Cancer Res. 1983 Oct;43(10):4643–4647. [PubMed] [Google Scholar]
  3. Burlington H., Cronkite E. P., Laissue J. A., Reincke U., Shadduck R. K. Colony-stimulating activity in cultures of granulocytosis-inducing tumor. Proc Soc Exp Biol Med. 1977 Jan;154(1):86–92. doi: 10.3181/00379727-154-19610. [DOI] [PubMed] [Google Scholar]
  4. Lee M. Y., Sperlin A., Dale D. C. Distribution of granulocytopoietic committed stem cells in mice with tumor induced neutrophilia. Exp Hematol. 1980 Mar;8(3):249–255. [PubMed] [Google Scholar]
  5. Lollini P. L., de Giovanni C., Eusebi V., Nicoletti G., Prodi G., Nanni P. High-metastatic clones selected in vitro from a recent spontaneous BALB/c mammary adenocarcinoma cell line. Clin Exp Metastasis. 1984 Jul-Sep;2(3):251–259. doi: 10.1007/BF00132932. [DOI] [PubMed] [Google Scholar]
  6. Milas L., Faykus M. H., Jr, McBride W. H., Hunter N., Peters L. J. Concomitant development of granulocytosis and enhancement of metastases formation in tumor-bearing mice. Clin Exp Metastasis. 1984 Jul-Sep;2(3):181–190. doi: 10.1007/BF00132924. [DOI] [PubMed] [Google Scholar]
  7. Mizoguchi H., Suda T., Miura Y., Kubota K., Takaku F. Hemopoietic stem cells in nude mice transplanted with colony-stimulating-factor-producing tumors. Exp Hematol. 1982 Nov;10(10):874–880. [PubMed] [Google Scholar]
  8. Nanni P., de Giovanni C., Lollini P. L., Nicoletti G., Prodi G. TS/A: a new metastasizing cell line from a BALB/c spontaneous mammary adenocarcinoma. Clin Exp Metastasis. 1983 Oct-Dec;1(4):373–380. doi: 10.1007/BF00121199. [DOI] [PubMed] [Google Scholar]
  9. Okabe T., Fujisawa M., Kudo H., Honma H., Ohsawa N., Takaku F. Establishment of a human colony-stimulating-factor-producing cell line from an undifferentiated large cell carcinoma of the lung. Cancer. 1984 Sep 15;54(6):1024–1029. doi: 10.1002/1097-0142(19840915)54:6<1024::aid-cncr2820540616>3.0.co;2-1. [DOI] [PubMed] [Google Scholar]
  10. Okabe T., Nomura H., Oshawa N. Establishment and characterization of a human colony-stimulating factor-producing cell line from a squamous cell carcinoma of the thyroid gland. J Natl Cancer Inst. 1982 Dec;69(6):1235–1243. [PubMed] [Google Scholar]
  11. Okabe T., Sato N., Kondo Y., Asano S., Ohsawa N., Kosaka K., Ueyama Y. Establishment and characterization of a human cancer cell line that produces human colony-stimulating factor. Cancer Res. 1978 Nov;38(11 Pt 1):3910–3917. [PubMed] [Google Scholar]
  12. Pessina A., Eridani S., Brambilla P., Cattoretti G., Marocchi A., Mocarelli P. Effect of tumor-related factors on the in vivo and in vitro colony-forming ability of normal mouse marrow cells. Neoplasma. 1981;28(5):541–548. [PubMed] [Google Scholar]
  13. Prystowsky M. B., Naujokas M. F., Ihle J. N., Goldwasser E., Fitch F. W. A microassay for colony-stimulating factor based on thymidine incorporation. Am J Pathol. 1984 Jan;114(1):149–156. [PMC free article] [PubMed] [Google Scholar]
  14. Reincke U., Burlington H., Carsten A. L., Cronkite E. P., Laissue J. A. Hemopoietic effects in mice of a transplanted, granulocytosis-inducing tumor. Exp Hematol. 1978 Apr;6(4):421–430. [PubMed] [Google Scholar]
  15. Sato N., Asano S., Ueyama Y., Mori M., Okabe T., Kondo Y., Ohsawa N., Kosaka K. Granulocytosis and colony-stimulating activity (CSA) produced by a human squamous cell carcinoma. Cancer. 1979 Feb;43(2):605–610. doi: 10.1002/1097-0142(197902)43:2<605::aid-cncr2820430230>3.0.co;2-p. [DOI] [PubMed] [Google Scholar]
  16. Sato N., Michaelides M. C., Wallack M. K. Characterization of tumorigenicity, mortality, metastasis, and splenomegaly of two cultured murine colon lines. Cancer Res. 1981 Jun;41(6):2267–2272. [PubMed] [Google Scholar]
  17. Suda T., Miura Y., Mizoguchi H., Kubota K., Takaku F. A case of lung cancer associated with granulocytosis and production of colony-stimulating activity by the tumour. Br J Cancer. 1980 Jun;41(6):980–984. doi: 10.1038/bjc.1980.177. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takeda A., Suzumori K., Sugimoto Y., Yagami Y., Miyazawa T., Yamada C., Matsuyama M. Clear cell carcinoma of the ovary with colony-stimulating-factor production. Occurrence of marked granulocytosis in a patient and nude mice. Cancer. 1984 Sep 15;54(6):1019–1023. doi: 10.1002/1097-0142(19840915)54:6<1019::aid-cncr2820540615>3.0.co;2-r. [DOI] [PubMed] [Google Scholar]

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