Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1985 Nov;52(5):747–755. doi: 10.1038/bjc.1985.253

Effect of host immune status on the spontaneous metastasis of cloned cell lines of the 13762NF rat mammary adenocarcinoma.

S M North, G L Nicolson
PMCID: PMC1977231  PMID: 4063149

Abstract

The importance of host immune status on the spontaneous metastasis of cloned cell lines of the 13762NF rat mammary adenocarcinoma was examined. Cell lines MTLn3 (high metastatic potential), MTF7 and MTLn2 (intermediate metastatic potential) and MTC (low metastatic potential) were subjected to a series of in vivo assays designed to assess how manipulation of the immune system in the syngeneic F344 host would affect the ability of these cells to metastasise. Treatment of tumour bearing rats with the immunosuppressive agents cyclosporin A or cyclophosphamide had little influence on metastasis in this system. Growth of tumours in congenitally athymic nude rats resulted in reduction of observed metastases. In addition, humoral immune response was not detectable during a 23-day period of tumour growth in F344 rats. Excision of the tumour growing in situ reduced the number of metastases when the tumours were resected early (less than 10 days), but at later times tumour resection did not influence the incidence of metastasis. The importance of initial lymphatic rather than haematogenous routes of dissemination was confirmed in experiments where the draining inguinal and axillary lymph nodes were removed at different times either before, or after, subcutaneous mammary fat pad injection of metastatic tumour cells.

Full text

PDF
747

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander P. Back to the drawing board--the need for more realistic model systems for immunotherapy. Cancer. 1977 Jul;40(1 Suppl):467–470. doi: 10.1002/1097-0142(197707)40:1+<467::aid-cncr2820400709>3.0.co;2-6. [DOI] [PubMed] [Google Scholar]
  2. Borel J. F., Feurer C., Magnée C., Stähelin H. Effects of the new anti-lymphocytic peptide cyclosporin A in animals. Immunology. 1977 Jun;32(6):1017–1025. [PMC free article] [PubMed] [Google Scholar]
  3. Dean C. J., Hobbs S. M., Hopkins J. U., North S. M., Styles J. M. Syngeneic antitumour antibodies in rats: clearance of cell-bound antibody in vivo and in vitro. Br J Cancer. 1982 Aug;46(2):190–197. doi: 10.1038/bjc.1982.183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eccles S. A., Heckford S. E., Alexander P. Effect of cyclosporin A on the growth and spontaneous metastasis of syngeneic animal tumours. Br J Cancer. 1980 Aug;42(2):252–259. doi: 10.1038/bjc.1980.224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eccles S. A., Styles J. M., Hobbs S. M., Dean C. J. Metastasis in the nude rat associated with lack of immune response. Br J Cancer. 1979 Nov;40(5):802–805. doi: 10.1038/bjc.1979.264. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Festing M. F., May D., Connors T. A., Lovell D., Sparrow S. An athymic nude mutation in the rat. Nature. 1978 Jul 27;274(5669):365–366. doi: 10.1038/274365a0. [DOI] [PubMed] [Google Scholar]
  7. Fogel M., Gorelik E., Segal S., Feldman M. Differences in cell surface antigens of tumor metastases and those of the local tumor. J Natl Cancer Inst. 1979 Mar;62(3):585–588. doi: 10.1093/jnci/62.3.585. [DOI] [PubMed] [Google Scholar]
  8. Freireich E. J., Gehan E. A., Rall D. P., Schmidt L. H., Skipper H. E. Quantitative comparison of toxicity of anticancer agents in mouse, rat, hamster, dog, monkey, and man. Cancer Chemother Rep. 1966 May;50(4):219–244. [PubMed] [Google Scholar]
  9. Hanna N. Inhibition of experimental tumor metastasis by selective activation of natural killer cells. Cancer Res. 1982 Apr;42(4):1337–1342. [PubMed] [Google Scholar]
  10. Haskill J. S., Key M., Radov L. A., Parthenais E., Korn J. H., Fett J. W., Yamamura Y., DeLustro F., Vesley J., Gant G. The importance of antibody and macrophages in spontaneous and drug-induced regression of the T1699 mammary adenocarcinoma. J Reticuloendothel Soc. 1979 Oct;26(4):417–425. [PubMed] [Google Scholar]
  11. Herberman R. B., Nunn M. E., Holden H. T., Lavrin D. H. Natural cytotoxic reactivity of mouse lymphoid cells against syngeneic and allogeneic tumors. II. Characterization of effector cells. Int J Cancer. 1975 Aug 15;16(2):230–239. doi: 10.1002/ijc.2910160205. [DOI] [PubMed] [Google Scholar]
  12. Mantovani A., Jerrells T. R., Dean J. H., Herberman R. B. Cytolytic and cytostatic activity on tumor cells of circulating human monocytes. Int J Cancer. 1979 Jan 15;23(1):18–27. doi: 10.1002/ijc.2910230105. [DOI] [PubMed] [Google Scholar]
  13. Neri A., Welch D., Kawaguchi T., Nicolson G. L. Development and biologic properties of malignant cell sublines and clones of a spontaneously metastasizing rat mammary adenocarcinoma. J Natl Cancer Inst. 1982 Mar;68(3):507–517. [PubMed] [Google Scholar]
  14. Nicolson G. L., Poste G. Tumor cell diversity and host responses in cancer metastasis--part I--properties of metastatic cells. Curr Probl Cancer. 1982 Dec;7(6):1–83. doi: 10.1016/s0147-0272(82)80017-2. [DOI] [PubMed] [Google Scholar]
  15. North S. M., Nicolson G. L. Heterogeneity in the sensitivities of the 13762NF rat mammary adenocarcinoma cell clones to cytolysis mediated by extra- and intratumoral macrophages. Cancer Res. 1985 Apr;45(4):1453–1458. [PubMed] [Google Scholar]
  16. North S. M., Styles J. M., Hobbs S. M., Dean C. J. Monoclonal antibodies to rat sarcomata. I. Immunization procedures and source of lymphoid cells for hybridoma production. Immunology. 1982 Nov;47(3):397–405. [PMC free article] [PubMed] [Google Scholar]
  17. Reading C. L., Kraemer P. M., Miner K. M., Nicolson G. L. In vivo and in vitro properties of malignant variants of RAW117 metastatic murine lymphoma/lymphosarcoma. Clin Exp Metastasis. 1983 Apr-Jun;1(2):135–151. doi: 10.1007/BF00121493. [DOI] [PubMed] [Google Scholar]
  18. Robins R. A., Baldwin R. W. Tumour-specific antibody neutralization of factors in rat hepatoma-bearer serum which abrogate lymph-node-cell cytotoxicity. Int J Cancer. 1974 Nov 15;14(5):589–597. doi: 10.1002/ijc.2910140505. [DOI] [PubMed] [Google Scholar]
  19. Schirrmacher V., Bosslet K., Shantz G., Clauer K., Hübsch D. Tumor metastases and cell-mediated immunity in a model system in DBA/2 mice. IV. Antigenic differences between a metastasizing variant and the parental tumor line revealed by cytotoxic T lymphocytes. Int J Cancer. 1979 Feb;23(2):245–252. doi: 10.1002/ijc.2910230216. [DOI] [PubMed] [Google Scholar]
  20. Shevach E. M. The effects of cyclosporin A on the immune system. Annu Rev Immunol. 1985;3:397–423. doi: 10.1146/annurev.iy.03.040185.002145. [DOI] [PubMed] [Google Scholar]
  21. Shin H. S., Economou J. S., Pasternack G. R., Johnson R. J., Hayden M. L. Antibody-mediated suppression of grafted lymphoma. IV. Influence of time of tumor residency in vivo and tumor size upon the effectiveness of suppression by syngeneic antibody. J Exp Med. 1976 Nov 2;144(5):1274–1283. doi: 10.1084/jem.144.5.1274. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Steck P. A., Nicolson G. L. Cell surface properties of spontaneously metastasizing rat mammary adenocarcinoma cell clones. Transplant Proc. 1984 Apr;16(2):355–360. [PubMed] [Google Scholar]
  23. Stutman O., Dien P., Wisun R. E., Lattime E. C. Natural cytotoxic cells against solid tumors in mice: blocking of cytotoxicity by D-mannose. Proc Natl Acad Sci U S A. 1980 May;77(5):2895–2898. doi: 10.1073/pnas.77.5.2895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Vaage J. Humoral and cellular immune factors in the systemic control of artifically induced metastases in C3Hf mice. Cancer Res. 1973 Aug;33(8):1957–1965. [PubMed] [Google Scholar]
  25. Welch D. R., Milas L., Tomasovic S. P., Nicolson G. L. Heterogeneous response and clonal drift of sensitivities of metastatic 13762NF mammary adenocarcinoma clones to gamma-radiation in vitro. Cancer Res. 1983 Jan;43(1):6–10. [PubMed] [Google Scholar]
  26. Welch D. R., Nicolson G. L. Phenotypic drift and heterogeneity in response of metastatic mammary adenocarcinoma cell clones to adriamycin, 5-fluoro-2'-deoxyuridine and methotrexate treatment in vitro. Clin Exp Metastasis. 1983 Oct-Dec;1(4):317–325. doi: 10.1007/BF00121194. [DOI] [PubMed] [Google Scholar]
  27. Woodruff M. F., Hodson B. A. The effect of passage in vitro and in vivo on the properties of murine fibrosarcomas I. Tumorigenicity and immunogenicity. Br J Cancer. 1985 Feb;51(2):161–169. doi: 10.1038/bjc.1985.25. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES