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. 1985 Oct;52(4):565–573. doi: 10.1038/bjc.1985.228

DNA index of ovarian carcinomas from 56 patients: in vivo in vitro studies.

E Erba, M Vaghi, S Pepe, G Amato, M Bistolfi, P Ubezio, C Mangioni, F Landoni, L Morasca
PMCID: PMC1977253  PMID: 4063134

Abstract

Out of 130 ovarian cancer patients the DNA index of cells from ovarian carcinoma was studied in 56 cases in which cytospin preparations showed the presence of atypical cells. In 24 patients the population had a diploid DNA index (1.0) and in the others the DNA index ranged from 1.2 to 2.0 (tetraploid). No hypodiploid or hypertetraploid populations were detected. Repeated samples from the same patients did not show any significant differences and primary culture did not alter the DNA index. In contrast, cell cycle phase distribution differed greatly from sample to sample, as also the ratio between DNA diploid and DNA aneuploid populations. Primary culture was successful in 57% of the tumours, with a higher percentage of success in DNA aneuploid tumours. After primary culture the ratio between DNA aneuploid cells and DNA diploid cells increased. In relation to the histological gradings of malignancy, DNA aneuploid cells clustered in the highest grade of malignancy. The mean S-phase for tumours with a DNA index of 1.0 was 3.5 and 14.1% for those with DNA index greater than 1. Ovarian carcinomas show a large difference in DNA index between patients even after primary culture.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkin N. B., Kay R. Prognostic significance of modal DNA value and other factors in malignant tumours, based on 1465 cases. Br J Cancer. 1979 Aug;40(2):210–221. doi: 10.1038/bjc.1979.168. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baisch H., Göhde W., Linden W. A. Analysis of PCP-data to determine the fraction of cells in the various phases of cell cycle. Radiat Environ Biophys. 1975 Jun 13;12(1):31–39. doi: 10.1007/BF02339807. [DOI] [PubMed] [Google Scholar]
  3. Baisch H., Otto U., König K., Klöppel G., Köllermann M., Linden W. A. DNA content of human kidney carcinoma cells in relation to histological grading. Br J Cancer. 1982 Jun;45(6):878–886. doi: 10.1038/bjc.1982.140. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barlogie B., Drewinko B., Schumann J., Göhde W., Dosik G., Latreille J., Johnston D. A., Freireich E. J. Cellular DNA content as a marker of neoplasia in man. Am J Med. 1980 Aug;69(2):195–203. doi: 10.1016/0002-9343(80)90379-4. [DOI] [PubMed] [Google Scholar]
  5. Barlogie B., Göhde W., Drewinko B. Flow cytometric analysis of DNA content for ploidy determination in human solid tumors. J Histochem Cytochem. 1979 Jan;27(1):505–507. doi: 10.1177/27.1.374615. [DOI] [PubMed] [Google Scholar]
  6. Barlogie B., Göhde W., Johnston D. A., Smallwood L., Schumann J., Drewinko B., Freireich E. J. Determination of ploidy and proliferative characteristics of human solid tumors by pulse cytophotometry. Cancer Res. 1978 Oct;38(10):3333–3339. [PubMed] [Google Scholar]
  7. Barlogie B., Raber M. N., Schumann J., Johnson T. S., Drewinko B., Swartzendruber D. E., Göhde W., Andreeff M., Freireich E. J. Flow cytometry in clinical cancer research. Cancer Res. 1983 Sep;43(9):3982–3997. [PubMed] [Google Scholar]
  8. Bunn P. A., Jr, Krasnow S., Makuch R. W., Schlam M. L., Schechter G. P. Flow cytometric analysis of DNA content of bone marrow cells in patients with plasma cell myeloma: clinical implications. Blood. 1982 Mar;59(3):528–535. [PubMed] [Google Scholar]
  9. Diamond L. W., Nathwani B. N., Rappaport H. Flow cytometry in the diagnosis and classification of malignant lymphoma and leukemia. Cancer. 1982 Sep 15;50(6):1122–1135. doi: 10.1002/1097-0142(19820915)50:6<1122::aid-cncr2820500616>3.0.co;2-q. [DOI] [PubMed] [Google Scholar]
  10. Erba E., Ubezio P., Colombo T., Broggini M., Torti L., Vaghi M., D'Incalci M., Morasca L. Flow-cytometric analysis of DNA distribution after VP16-213 treatment of Lewis lung carcinoma. Cancer Chemother Pharmacol. 1983;10(3):208–211. doi: 10.1007/BF00255765. [DOI] [PubMed] [Google Scholar]
  11. Friedlander M. L., Hedley D. W., Taylor I. W., Russell P., Coates A. S., Tattersall M. H. Influence of cellular DNA content on survival in advanced ovarian cancer. Cancer Res. 1984 Jan;44(1):397–400. [PubMed] [Google Scholar]
  12. Friedlander M. L., Taylor I. W., Russell P., Musgrove E. A., Hedley D. H., Tattersall M. H. Ploidy as a prognostic factor in ovarian cancer. Int J Gynecol Pathol. 1983;2(1):55–63. doi: 10.1097/00004347-198301000-00005. [DOI] [PubMed] [Google Scholar]
  13. Friedlander M. L., Taylor I. W., Russell P., Tattersall M. H. Cellular DNA content--a stable feature in epithelial ovarian cancer. Br J Cancer. 1984 Feb;49(2):173–179. doi: 10.1038/bjc.1984.29. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Haskill S., Becker S., Fowler W., Walton L. Mononuclear-cell infiltration in ovarian cancer. I. Inflammatory-cell infiltrates from tumour and ascites material. Br J Cancer. 1982 May;45(5):728–736. doi: 10.1038/bjc.1982.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Haskill S., Kivinen S., Nelson K., Fowler W. C., Jr Detection of intratumor heterogeneity by simultaneous multiparameter flow cytometric analysis with enzyme and DNA markers. Cancer Res. 1983 Mar;43(3):1003–1009. [PubMed] [Google Scholar]
  16. Laerum O. D., Farsund T. Clinical application of flow cytometry: a review. Cytometry. 1981 Jul;2(1):1–13. doi: 10.1002/cyto.990020102. [DOI] [PubMed] [Google Scholar]
  17. Lau C. C., Pardee A. B. Mechanism by which caffeine potentiates lethality of nitrogen mustard. Proc Natl Acad Sci U S A. 1982 May;79(9):2942–2946. doi: 10.1073/pnas.79.9.2942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Long M. E., Sommers S. C. Staging, grading and histochemistry of ovarian epithelial tumors. Clin Obstet Gynecol. 1969 Dec;12(4):937–954. doi: 10.1097/00003081-196912040-00007. [DOI] [PubMed] [Google Scholar]
  19. Maiolo A. T., Foa P., Mozzana R., Chiorboli O., Ciani A., Maisto A., Starace G. Flow cytometric analysis of cellular DNA in human acute nonlymphatic leukemias and dysmyelopoietic syndromes. Cytometry. 1982 Jan;2(4):265–267. doi: 10.1002/cyto.990020411. [DOI] [PubMed] [Google Scholar]
  20. Mantovani A., Peri G., Polentarutti N., Bolis G., Mangioni C., Spreafico F. Effects on in vitro tumor growth of macrophages isolated from human ascitic ovarian tumors. Int J Cancer. 1979 Feb;23(2):157–164. doi: 10.1002/ijc.2910230204. [DOI] [PubMed] [Google Scholar]
  21. Ozols R. F., Garvin A. J., Costa J., Simon R. M., Young R. C. Advanced ovarian cancer: correlation of histologic grade with response to therapy and survival. Cancer. 1980 Feb;45(3):572–581. doi: 10.1002/1097-0142(19800201)45:3<572::aid-cncr2820450325>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
  22. Recchia M., Rocchetti M. The simulated randomization test. Comput Programs Biomed. 1982 Oct;15(2):111–116. doi: 10.1016/0010-468x(82)90062-9. [DOI] [PubMed] [Google Scholar]
  23. Teodori L., Tirindelli-Danesi D., Mauro F., De Vita R., Uccelli R., Botti C., Modini C., Nervi C., Stipa S. Non-small-cell lung carcinoma: tumor characterization on the basis of flow cytometrically determined cellular heterogeneity. Cytometry. 1983 Sep;4(2):174–183. doi: 10.1002/cyto.990040212. [DOI] [PubMed] [Google Scholar]
  24. Von Hoff D. D., Cowan J., Harris G., Reisdorf G. Human tumor cloning: feasibility and clinical correlations. Cancer Chemother Pharmacol. 1981;6(3):265–271. doi: 10.1007/BF00256979. [DOI] [PubMed] [Google Scholar]
  25. Wilson A. P., Neal F. E. In vitro sensitivity of human ovarian tumours to chemotherapeutic agents. Br J Cancer. 1981 Aug;44(2):189–200. doi: 10.1038/bjc.1981.170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. van Haaften-Day C., Russell P., Rugg C., Wills E. J., Tattersall M. H. Flow cytometric and morphological studies of ovarian carcinoma cell lines and xenografts. Cancer Res. 1983 Aug;43(8):3725–3731. [PubMed] [Google Scholar]

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