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. 1991 Jul;64(1):164–168. doi: 10.1038/bjc.1991.262

Raised levels of latent collagenase activating angiogenesis factor (ESAF) are present in actively growing human intracranial tumours.

C M Taylor 1, J B Weiss 1, R H Lye 1
PMCID: PMC1977311  PMID: 1649618

Abstract

Endothelial cell stimulating angiogenesis factor (ESAF) is a potent, low molecular mass mitogen, specific for endothelial cells. In common with various protein growth factors, it displays angiogenic activity in a variety of biological test systems. However, it differs from these other factors by virtue of its low molecular mass and its ability to activate latent matrix metalloproteinases in a dose dependent manner. This activity has been used to quantify the factor in both normal and diseased brain tissue. The concentration of ESAF determined in biopsies from different types of intracranial tumours varied: in some tumour types the level was close to that of control samples whereas in others it rose to levels comparable to those encountered in the pineal gland, the richest source of ESAF in mature mammals. Tumours considered to be benign contained significantly less ESAF than those neoplasms classified as being malignant (P = 0.025). There was also a correlation between the mitotic activity of tumour samples, as determined by conventional H & E histochemical staining and the ESAF concentration present. These findings agree with previous studies in which elevated ESAF levels have been found in tissue where proliferation of vascular elements has been observed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bostwick D. G., Roth K. A., Evans C. J., Barchas J. D., Bensch K. G. Gastrin-releasing peptide, a mammalian analog of bombesin, is present in human neuroendocrine lung tumors. Am J Pathol. 1984 Nov;117(2):195–200. [PMC free article] [PubMed] [Google Scholar]
  2. Coffey R. J., Jr, Shipley G. D., Moses H. L. Production of transforming growth factors by human colon cancer lines. Cancer Res. 1986 Mar;46(3):1164–1169. [PubMed] [Google Scholar]
  3. Elstow S. F., Schor A. M., Weiss J. B. Bovine retinal angiogenesis factor is a small molecule (molecular mass less than 600). Invest Ophthalmol Vis Sci. 1985 Jan;26(1):74–79. [PubMed] [Google Scholar]
  4. Folkman J., Klagsbrun M. Angiogenic factors. Science. 1987 Jan 23;235(4787):442–447. doi: 10.1126/science.2432664. [DOI] [PubMed] [Google Scholar]
  5. Gisslow M. T., McBride B. C. A rapid sensitive collagenase assay. Anal Biochem. 1975 Sep;68(1):70–78. doi: 10.1016/0003-2697(75)90680-6. [DOI] [PubMed] [Google Scholar]
  6. Klagsbrun M., Sasse J., Sullivan R., Smith J. A. Human tumor cells synthesize an endothelial cell growth factor that is structurally related to basic fibroblast growth factor. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2448–2452. doi: 10.1073/pnas.83.8.2448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Korf H. W., Czerwionka M., Reiner J., Schachenmayr W., Schalken J. J., de Grip W., Gery I. Immunocytochemical evidence of molecular photoreceptor markers in cerebellar medulloblastomas. Cancer. 1987 Oct 15;60(8):1763–1766. doi: 10.1002/1097-0142(19871015)60:8<1763::aid-cncr2820600815>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Lopez Pousa S., Vich i Pascuchi J. M., Ferrer I., Domenech J. M., Lopez Pousa A., Real Arribas F. Angiogenic activity in fluid samples from tumoral patients. Cancer. 1983 Oct 15;52(8):1365–1368. doi: 10.1002/1097-0142(19831015)52:8<1365::aid-cncr2820520805>3.0.co;2-i. [DOI] [PubMed] [Google Scholar]
  10. Odedra R., Weiss J. B. A synergistic effect on microvessel cell proliferation between basic fibroblast growth factor (FGFb) and endothelial cell stimulating angiogenesis factor (ESAF). Biochem Biophys Res Commun. 1987 Mar 30;143(3):947–953. doi: 10.1016/0006-291x(87)90342-1. [DOI] [PubMed] [Google Scholar]
  11. Schor A. M., Schor S. L., Weiss J. B., Brown R. A., Kumar S., Phillips P. Stimulation by a low-molecular-weight angiogenic factor of capillary endothelial cells in culture. Br J Cancer. 1980 May;41(5):790–799. doi: 10.1038/bjc.1980.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shing Y., Folkman J., Haudenschild C., Lund D., Crum R., Klagsbrun M. Angiogenesis is stimulated by a tumor-derived endothelial cell growth factor. J Cell Biochem. 1985;29(4):275–287. doi: 10.1002/jcb.240290402. [DOI] [PubMed] [Google Scholar]
  13. Taylor C. M., McLaughlin B., Weiss J. B., Smith I. Bovine and human pineal glands contain substantial quantities of endothelial cell stimulating angiogenic factor. J Neural Transm. 1988;71(1):79–84. doi: 10.1007/BF01259413. [DOI] [PubMed] [Google Scholar]
  14. Weiss J. B., Brown R. A., Kumar S., Phillips P. An angiogenic factor isolated from tumours: a potent low-molecular-weight compound. Br J Cancer. 1979 Sep;40(3):493–496. doi: 10.1038/bjc.1979.206. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weiss J. B., Hill C. R., Davis R. J., McLaughlin B., Sedowofia K. A., Brown R. A. Activation of a procollagenase by low-molecular-weight angiogenesis factor. Biosci Rep. 1983 Feb;3(2):171–177. doi: 10.1007/BF01121948. [DOI] [PubMed] [Google Scholar]
  16. Wharton J., Polak J. M., Bloom S. R., Ghatei M. A., Solcia E., Brown M. R., Pearse A. G. Bombesin-like immunoreactivity in the lung. Nature. 1978 Jun 29;273(5665):769–770. doi: 10.1038/273769a0. [DOI] [PubMed] [Google Scholar]

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