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. 1991 Nov;64(5):875–879. doi: 10.1038/bjc.1991.417

The detection of phthalocyanine fluorescence in normal rat bladder wall using sensitive digital imaging microscopy.

A J Pope 1, A J MacRobert 1, D Phillips 1, S G Bown 1
PMCID: PMC1977460  PMID: 1931609

Abstract

The ability to detect photosensitisers in tissue at a microscopical level is important when studying photodynamic therapy (PDT) in both normal and malignant tissue. We have studied the fluorescence distribution of aluminium sulphonated phthalocyanine (A1SPc) in the normal rat bladder using a cooled CCD (charge coupled device) imaging system with computerised image processing. This system makes it possible to carry out a quantitative assessment of photosensitiser fluorescence in the various layers of the bladder wall. The highest fluorescence intensities were obtained within 1 h of intravenous administration but there was little selectivity of uptake between layers. A1SPc was eliminated from the deeper muscle layers more quickly than from the superficial layers of the bladder wall so that by 24 h a 4:1 ratio of fluorescence intensity was apparent which persisted at least until 72 h, although the absolute amount of photosensitiser declined. Following irradiation by red light (675 nm), photobleaching of the sensitiser in the deeper layers further increased this ratio. Direct absorption of A1SPc by the bladder wall following intravesical administration proved unreliable.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barr H., Tralau C. J., MacRobert A. J., Krasner N., Boulos P. B., Clark C. G., Bown S. G. Photodynamic therapy in the normal rat colon with phthalocyanine sensitisation. Br J Cancer. 1987 Aug;56(2):111–118. doi: 10.1038/bjc.1987.166. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berg K., Bommer J. C., Moan J. Evaluation of sulfonated aluminum phthalocyanines for use in photochemotherapy. A study on the relative efficiencies of photoinactivation. Photochem Photobiol. 1989 May;49(5):587–594. doi: 10.1111/j.1751-1097.1989.tb08428.x. [DOI] [PubMed] [Google Scholar]
  3. Berns M. W., Hammer-Wilson M., Walter R. J., Wright W., Chow M. H., Nahabedian M., Wile A. Uptake and localization of HPD and "active fraction" in tissue culture and in serially biopsied human tumors. Prog Clin Biol Res. 1984;170:501–520. [PubMed] [Google Scholar]
  4. Bown S. G., Tralau C. J., Smith P. D., Akdemir D., Wieman T. J. Photodynamic therapy with porphyrin and phthalocyanine sensitisation: quantitative studies in normal rat liver. Br J Cancer. 1986 Jul;54(1):43–52. doi: 10.1038/bjc.1986.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harty J. I., Amin M., Wieman T. J., Tseng M. T., Ackerman D., Broghamer W. Complications of whole bladder dihematoporphyrin ether photodynamic therapy. J Urol. 1989 Jun;141(6):1341–1346. doi: 10.1016/s0022-5347(17)41302-4. [DOI] [PubMed] [Google Scholar]
  6. Mang T. S., Dougherty T. J., Potter W. R., Boyle D. G., Somer S., Moan J. Photobleaching of porphyrins used in photodynamic therapy and implications for therapy. Photochem Photobiol. 1987 Apr;45(4):501–506. doi: 10.1111/j.1751-1097.1987.tb05409.x. [DOI] [PubMed] [Google Scholar]
  7. Moan J. Effect of bleaching of porphyrin sensitizers during photodynamic therapy. Cancer Lett. 1986 Oct;33(1):45–53. doi: 10.1016/0304-3835(86)90100-x. [DOI] [PubMed] [Google Scholar]
  8. Nseyo U. O., Dougherty T. J., Sullivan L. Photodynamic therapy in the management of resistant lower urinary tract carcinoma. Cancer. 1987 Dec 15;60(12):3113–3119. doi: 10.1002/1097-0142(19871215)60:12<3113::aid-cncr2820601242>3.0.co;2-2. [DOI] [PubMed] [Google Scholar]
  9. Pope A. J., Bown S. G. The morphological and functional changes in rat bladder following photodynamic therapy with phthalocyanine photosensitization. J Urol. 1991 May;145(5):1064–1070. doi: 10.1016/s0022-5347(17)38536-1. [DOI] [PubMed] [Google Scholar]
  10. Potter W. R., Mang T. S., Dougherty T. J. The theory of photodynamic therapy dosimetry: consequences of photo-destruction of sensitizer. Photochem Photobiol. 1987 Jul;46(1):97–101. doi: 10.1111/j.1751-1097.1987.tb04741.x. [DOI] [PubMed] [Google Scholar]
  11. Profio A. E., Doiron D. R. Dose measurements in photodynamic therapy of cancer. Lasers Surg Med. 1987;7(1):1–5. doi: 10.1002/lsm.1900070102. [DOI] [PubMed] [Google Scholar]
  12. Spikes J. D., Bommer J. C. Zinc tetrasulphophthalocyanine as a photodynamic sensitizer for biomolecules. Int J Radiat Biol Relat Stud Phys Chem Med. 1986 Jul;50(1):41–45. doi: 10.1080/09553008614550421. [DOI] [PubMed] [Google Scholar]
  13. Tralau C. J., Barr H., Sandeman D. R., Barton T., Lewin M. R., Bown S. G. Aluminum sulfonated phthalocyanine distribution in rodent tumors of the colon, brain and pancreas. Photochem Photobiol. 1987 Nov;46(5):777–781. doi: 10.1111/j.1751-1097.1987.tb04847.x. [DOI] [PubMed] [Google Scholar]

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