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. 1991 Aug;64(2):288–292. doi: 10.1038/bjc.1991.293

Cis dichlorodiammine platinum induced DNA interstrand cross-links in primary cultures of human ovarian cancer.

G Balconi 1, Y Pang 1, M Broggini 1, F Morali 1, M Marzola 1, E Erba 1, M Ponti 1, L Spinelli 1, C Mangioni 1, L Redaelli 1, et al.
PMCID: PMC1977530  PMID: 1892757

Abstract

We quantified and examined the kinetics of DNA interstrand cross links (DNA-ISC) caused by Cis dichlorodiammine platinum (DDP) using the method of alkaline elution in 58 highly purified human ovarian tumours growing in primary culture. A large heterogeneity in both the quantity and kinetics of DDP induced DNA-ISC was observed in cultures derived from neoplasms of different patients and from different lesions of the same patient. In the majority of cases. DNA-ISC lasted for prolonged time intervals after 1 h drug exposure, being significantly repaired only 48 or 72 h following drug washout. The persistence of DNA-ISC is probably due to a prolonged formation of these lesions for up to 24 h as assessed by the change in the repair kinetics that occurred after preventing new DNA-ISC formation by quenching of monoadducts with thiourea. The inefficient repair of DDP monoadducts appears therefore to be a possible reason for the permanence of DNA-ISC. These studies suggest that the long permanence of DNA-ISC in human ovarian cancer could be the basis for the high selectivity of DDP for this human malignancy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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