Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1992 Apr;65(4):491–497. doi: 10.1038/bjc.1992.102

Non-invasive monitoring of photodynamic therapy with 99technetium HMPAO scintigraphy.

R B Moore 1, J D Chapman 1, A D Mokrzanowski 1, M R Arnfield 1, M S McPhee 1, A J McEwan 1
PMCID: PMC1977552  PMID: 1562457

Abstract

The effect of photodynamic therapy (PDT) on tumour perfusion in both anaplastic (R3327-AT) and well differentiated (R3327-H) Dunning prostatic tumours was studied using the radiopharmaceutical 99Technetium hexamethylpropyleneamine oxime (99mTc-HMPAO). Tumours in the left flanks of rats (Copenhage x Fischer, F1 hybrids) were treated with interstitial PDT when their volumes reached 2-3 cm3. Qualitative and quantitative data from pre- and post-PDT scintigraphy revealed a light-dose-dependent shut-down of tumour perfusion which was also time-dependent. Maximal shut-down, following a 1,600 J light-dose, occurred about 8 h post-PDT. Light exposure 2 h after the intravenous administration of the photosensitiser (Photofrin II) produced a greater vascular shut-down than did light exposure 24 h after the administration of the drug. Regional differences in perfusion within treated and non-treated tumours were measured by tomographic procedures. Light-dose-dependent volumes of perfusion shut-down were demonstrated in addition to the naturally occurring regional differences in tumour perfusion. This radiopharmaceutical may have future utility for monitoring the clinical treatment of solid tumours with PDT.

Full text

PDF
491

Images in this article

493Figure 1
on p.493
494Figure 2
on p.494
495Figure 3
on p.495
496Figure 6
on p.496

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnfield M. R., Tulip J., Chetner M., McPhee M. S. Optical dosimetry for interstitial photodynamic therapy. Med Phys. 1989 Jul-Aug;16(4):602–608. doi: 10.1118/1.596361. [DOI] [PubMed] [Google Scholar]
  2. Arnfield M., Gonzalez S., Lea P., Tulip J., McPhee M. Cylindrical irradiator fiber tip for photodynamic therapy. Lasers Surg Med. 1986;6(2):150–154. doi: 10.1002/lsm.1900060211. [DOI] [PubMed] [Google Scholar]
  3. Ballinger J. R., Reid R. H., Gulenchyn K. Y. Radiochemical purity of [99mTc]HM-PAO. J Nucl Med. 1988 Apr;29(4):572–573. [PubMed] [Google Scholar]
  4. Berns M. W., Dahlman A., Johnson F. M., Burns R., Sperling D., Guiltinan M., Siemens A., Walter R., Wright W., Hammer-Wilson M. In vitro cellular effects of hematoporphyrin derivative. Cancer Res. 1982 Jun;42(6):2325–2329. [PubMed] [Google Scholar]
  5. Bertoloni G., Salvato B., Dall'Acqua M., Vazzoler M., Jori G. Hematoporphyrin-sensitized photoinactivation of Streptococcus faecalis. Photochem Photobiol. 1984 Jun;39(6):811–816. doi: 10.1111/j.1751-1097.1984.tb08864.x. [DOI] [PubMed] [Google Scholar]
  6. Chapman J. D., Stobbe C. C., Arnfield M. R., Santus R., McPhee M. S. The effectiveness of short-term versus long-term exposures to Photofrin II in killing light-activated tumor cells. Radiat Res. 1991 Oct;128(1):82–89. [PubMed] [Google Scholar]
  7. DUNNING W. F. PROSTATE CANCER IN THE RAT. Natl Cancer Inst Monogr. 1963 Oct;12:351–369. [PubMed] [Google Scholar]
  8. Fingar V. H., Wieman T. J., Doak K. W. Role of thromboxane and prostacyclin release on photodynamic therapy-induced tumor destruction. Cancer Res. 1990 May 1;50(9):2599–2603. [PubMed] [Google Scholar]
  9. Gonzalez S., Arnfield M. R., Meeker B. E., Tulip J., Lakey W. H., Chapman J. D., McPhee M. S. Treatment of Dunning R3327-AT rat prostate tumors with photodynamic therapy in combination with misonidazole. Cancer Res. 1986 Jun;46(6):2858–2862. [PubMed] [Google Scholar]
  10. Henderson B. W., Donovan J. M. Release of prostaglandin E2 from cells by photodynamic treatment in vitro. Cancer Res. 1989 Dec 15;49(24 Pt 1):6896–6900. [PubMed] [Google Scholar]
  11. Henderson B. W., Waldow S. M., Mang T. S., Potter W. R., Malone P. B., Dougherty T. J. Tumor destruction and kinetics of tumor cell death in two experimental mouse tumors following photodynamic therapy. Cancer Res. 1985 Feb;45(2):572–576. [PubMed] [Google Scholar]
  12. Henry J. M., Isaacs J. T. Synergistic enhancement of the efficacy of the bioreductively activated alkylating agent RSU-1164 in the treatment of prostatic cancer by photodynamic therapy. J Urol. 1989 Jul;142(1):165–170. doi: 10.1016/s0022-5347(17)38704-9. [DOI] [PubMed] [Google Scholar]
  13. Hilf R., Gibson S. L., Penney D. P., Ceckler T. L., Bryant R. G. Early biochemical responses to photodynamic therapy monitored by NMR spectroscopy. Photochem Photobiol. 1987 Nov;46(5):809–817. doi: 10.1111/j.1751-1097.1987.tb04852.x. [DOI] [PubMed] [Google Scholar]
  14. Hirsch B. D., Walz N. C., Meeker B. E., Arnfield M. R., Tulip J., McPhee M. S., Chapman J. D. Photodynamic therapy-induced hypoxia in rat tumors and normal tissues. Photochem Photobiol. 1987 Nov;46(5):847–852. doi: 10.1111/j.1751-1097.1987.tb04858.x. [DOI] [PubMed] [Google Scholar]
  15. Mador D., Ritchie B., Meeker B., Moore R., Elliott F. G., McPhee M. S., Chapman J. D., Lakey W. H. Response of the Dunning R3327H prostatic adenocarcinoma to radiation and various chemotherapeutic drugs. Cancer Treat Rep. 1982 Oct;66(10):1837–1843. [PubMed] [Google Scholar]
  16. Mitchell J. B., McPherson S., DeGraff W., Gamson J., Zabell A., Russo A. Oxygen dependence of hematoporphyrin derivative-induced photoinactivation of Chinese hamster cells. Cancer Res. 1985 May;45(5):2008–2011. [PubMed] [Google Scholar]
  17. Moan J., Sommer S. Oxygen dependence of the photosensitizing effect of hematoporphyrin derivative in NHIK 3025 cells. Cancer Res. 1985 Apr;45(4):1608–1610. [PubMed] [Google Scholar]
  18. Reyftmann J. P., Kohen E., Morliere P., Santus R., Kohen C., Mangel W. F., Dubertret L., Hirschberg J. G. A microspectrofluorometric study of porphyrin-photosensitized single living cells--I. Membrane alterations. Photochem Photobiol. 1986 Oct;44(4):461–469. doi: 10.1111/j.1751-1097.1986.tb04693.x. [DOI] [PubMed] [Google Scholar]
  19. Rowell N. P., McCready V. R., Tait D., Flower M. A., Cronin B., Adams G. E., Horwich A. Technetium-99m HMPAO and SPECT in the assessment of blood flow in human lung tumours. Br J Cancer. 1989 Jan;59(1):135–141. doi: 10.1038/bjc.1989.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sandberg S., Romslo I., Høvding G., Bjørndal T. Porphyrin-induced photodamage as related to the subcellular localization of the porphyrins. Acta Derm Venereol Suppl (Stockh) 1982;100:75–80. [PubMed] [Google Scholar]
  21. Selman S. H., Kreimer-Birnbaum M., Klaunig J. E., Goldblatt P. J., Keck R. W., Britton S. L. Blood flow in transplantable bladder tumors treated with hematoporphyrin derivative and light. Cancer Res. 1984 May;44(5):1924–1927. [PubMed] [Google Scholar]
  22. Singh G., Jeeves W. P., Wilson B. C., Jang D. Mitochondrial photosensitization by Photofrin II. Photochem Photobiol. 1987 Nov;46(5):645–649. doi: 10.1111/j.1751-1097.1987.tb04826.x. [DOI] [PubMed] [Google Scholar]
  23. Star W. M., Marijnissen H. P., van den Berg-Blok A. E., Versteeg J. A., Franken K. A., Reinhold H. S. Destruction of rat mammary tumor and normal tissue microcirculation by hematoporphyrin derivative photoradiation observed in vivo in sandwich observation chambers. Cancer Res. 1986 May;46(5):2532–2540. [PubMed] [Google Scholar]
  24. Thorndyke C., Meeker B. E., Thomas G., Lakey W. H., McPhee M. S., Chapman J. D. The radiation sensitivities of R3327-H and R3327-AT rat prostate adenocarcinomas. J Urol. 1985 Jul;134(1):191–198. doi: 10.1016/s0022-5347(17)47055-8. [DOI] [PubMed] [Google Scholar]
  25. Torinuki W., Miura T., Seiji M. Lysosome destruction and lipoperoxide formation due to active oxygen generated from haematoporphyrin and UV irradiation. Br J Dermatol. 1980 Jan;102(1):17–27. doi: 10.1111/j.1365-2133.1980.tb05667.x. [DOI] [PubMed] [Google Scholar]
  26. Tromberg B. J., Kimel S., Orenstein A., Barker S. J., Hyatt J., Nelson J. S., Roberts W. G., Berns M. W. Tumor oxygen tension during photodynamic therapy. J Photochem Photobiol B. 1990 Apr 1;5(1):121–126. doi: 10.1016/1011-1344(90)85011-k. [DOI] [PubMed] [Google Scholar]
  27. Wieman T. J., Mang T. S., Fingar V. H., Hill T. G., Reed M. W., Corey T. S., Nguyen V. Q., Render E. R., Jr Effect of photodynamic therapy on blood flow in normal and tumor vessels. Surgery. 1988 Sep;104(3):512–517. [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES