Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1992 Apr;65(4):519–522. doi: 10.1038/bjc.1992.107

Induction of myogenic differentiation in human rhabdomyosarcoma cells by ionising radiation, N,N-dimethylformamide and their combination.

G Nicoletti 1, C De Giovanni 1, L Landuzzi 1, G Simone 1, P Rocchi 1, P Nanni 1, P L Lollini 1
PMCID: PMC1977576  PMID: 1562460

Abstract

Differentiation-inducing ability of gamma-radiation, N,N-dimethylformamide and their combination has been tested on human rhabdomyosarcoma RMZ-RC2 clone cells. Ionising radiation at 2-5 Gy doses induced a more differentiated morphology, with the appearance of an increased proportion of multinuclear myotube-like cells, and a significant increase in myosin-positive and multinuclear cells. Radiation appeared to act by inducing de novo differentiated elements. N,N-dimethylformamide was able to induce an increased myosin expression, but did not affect multinuclear cell proportion. The combined treatment (ionising radiation and N,N-dimethylformamide) resulted in an additive increase in the proportion of myosin-positive cells, approaching 25-35%, but de novo differentiated elements were not increased above the levels obtained with irradiation alone.

Full text

PDF
519

Images in this article

521Figure 2
on p.521

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aguanno S., Bouchè M., Adamo S., Molinaro M. 12-O-tetradecanoylphorbol-13-acetate-induced differentiation of a human rhabdomyosarcoma cell line. Cancer Res. 1990 Jun 1;50(11):3377–3382. [PubMed] [Google Scholar]
  2. Arundel C., Bock S., Brock W. A., Tofilon P. J. Radiosensitization of primary human tumor cell cultures by N-methylformamide. Int J Radiat Oncol Biol Phys. 1987 May;13(5):753–757. doi: 10.1016/0360-3016(87)90295-1. [DOI] [PubMed] [Google Scholar]
  3. Chen T. R. In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 stain. Exp Cell Res. 1977 Feb;104(2):255–262. doi: 10.1016/0014-4827(77)90089-1. [DOI] [PubMed] [Google Scholar]
  4. De Giovanni C., Nanni P., Nicoletti G., Ceccarelli C., Scotlandi K., Landuzzi L., Lollini P. L. Metastatic ability and differentiative properties of a new cell line of human embryonal rhabdomyosarcoma (CCA). Anticancer Res. 1989 Nov-Dec;9(6):1943–1949. [PubMed] [Google Scholar]
  5. Dexter D. L. N,N-Dimethylformamide-induced morphological differentiation and reduction of tumorigenicity in cultured mouse rhabdomyosarcoma cells. Cancer Res. 1977 Sep;37(9):3136–3140. [PubMed] [Google Scholar]
  6. Garvin A. J., Stanley W. S., Bennett D. D., Sullivan J. L., Sens D. A. The in vitro growth, heterotransplantation, and differentiation of a human rhabdomyosarcoma cell line. Am J Pathol. 1986 Oct;125(1):208–217. [PMC free article] [PubMed] [Google Scholar]
  7. Gerharz C. D., Gabbert H. E., Engers R., Ramp U., Mayer H., Luley C. Heterogeneous response to differentiation induction with different polar compounds in a clonal rat rhabdomyosarcoma cell line (BA-HAN-1C). Br J Cancer. 1989 Oct;60(4):578–584. doi: 10.1038/bjc.1989.317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Iwakawa M., Milas L., Hunter N., Tofilon P. J. Modification of tumor and normal tissue radioresponse in mice by N-methylformamide. Int J Radiat Oncol Biol Phys. 1987 Jan;13(1):55–60. doi: 10.1016/0360-3016(87)90260-4. [DOI] [PubMed] [Google Scholar]
  9. Kelland L. R., Bingle L., Edwards S., Steel G. G. High intrinsic radiosensitivity of a newly established and characterised human embryonal rhabdomyosarcoma cell line. Br J Cancer. 1989 Feb;59(2):160–164. doi: 10.1038/bjc.1989.34. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Leith J. T., Gaskins L. A., Dexter D. L., Calabresi P., Glicksman A. S. Alteration of the survival response of two human colon carcinoma subpopulations to x-irradiation by N,N-dimethylformamide. Cancer Res. 1982 Jan;42(1):30–34. [PubMed] [Google Scholar]
  11. Leith J. T., Lee E. S., Vayer A. J., Jr, Dexter D. L., Glicksman A. S. Enhancement of the responses of human colon adenocarcinoma cells to X-irradiation and cis-platinum by N-methylformamide (NMF). Int J Radiat Oncol Biol Phys. 1985 Nov;11(11):1971–1976. doi: 10.1016/0360-3016(85)90279-2. [DOI] [PubMed] [Google Scholar]
  12. Lollini P. L., De Giovanni C., Del Re B., Landuzzi L., Nicoletti G., Prodi G., Scotlandi K., Nanni P. Myogenic differentiation of human rhabdomyosarcoma cells induced in vitro by antineoplastic drugs. Cancer Res. 1989 Jul 1;49(13):3631–3636. [PubMed] [Google Scholar]
  13. Lollini P. L., De Giovanni C., Landuzzi L., Nicoletti G., Scotlandi K., Nanni P. Reduced metastatic ability of in vitro differentiated human rhabdomyosarcoma cells. Invasion Metastasis. 1991;11(2):116–124. [PubMed] [Google Scholar]
  14. Nanni P., Schiaffino S., De Giovanni C., Nicoletti G., Prodi G., Del Re B., Eusebi V., Ceccarelli C., Saggin L., Lollini P. L. RMZ: a new cell line from a human alveolar rhabdomyosarcoma. In vitro expression of embryonic myosin. Br J Cancer. 1986 Dec;54(6):1009–1014. doi: 10.1038/bjc.1986.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Reiss M., Gamba-Vitalo C., Sartorelli A. C. Induction of tumor cell differentiation as a therapeutic approach: preclinical models for hematopoietic and solid neoplasms. Cancer Treat Rep. 1986 Jan;70(1):201–218. [PubMed] [Google Scholar]
  16. Schiaffino S., Gorza L., Sartore S., Saggin L., Carli M. Embryonic myosin heavy chain as a differentiation marker of developing human skeletal muscle and rhabdomyosarcoma. A monoclonal antibody study. Exp Cell Res. 1986 Mar;163(1):211–220. doi: 10.1016/0014-4827(86)90574-4. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES