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. 1991 Oct;64(4):749–752. doi: 10.1038/bjc.1991.392

DNA aneuploidy and low S-phase fraction as favourable prognostic signs in metastatic melanoma.

T Muhonen 1, S Pyrhönen 1, A Laasonen 1, S Asko-Seljavaara 1, K Franssila 1
PMCID: PMC1977691  PMID: 1911224

Abstract

The prognostic value of cellular DNA content in melanoma metastases was investigated by flow cytometric analysis of fresh or paraffin-embedded tumour blocks from 95 consecutive patients referred to the Helsinki University Central Hospital Melanoma Team. Thirty-three per cent of the tumours were DNA diploid and 67% DNA aneuploid. S-phase fractions were lower in DNA diploid than in DNA aneuploid tumours (10.7% and 17.6%). Tumour ploidy and S-phase fraction were shown by multivariate Cox model analysis to be independent prognostic variables and major determinants of survival after first recurrence. Surprisingly, patients with DNA aneuploid tumours and with tumours with low SPF survived significantly longer than those with DNA diploid or high SPF tumours. This exceptional finding of favourable prognosis for DNA aneuploid tumours was more prominent among patients receiving intensive systemic therapy and among patients with stage IV disease, probably indicating a tendency for DNA aneuploid tumours to have higher sensitivity to systemic therapy.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abe S., Tsuneta Y., Makimura S., Itabashi K., Nagai T., Kawakami Y. Nuclear DNA content as an indicator of chemosensitivity in small-cell carcinoma of the lung. Anal Quant Cytol Histol. 1987 Oct;9(5):425–428. [PubMed] [Google Scholar]
  2. Breslow A. Thickness, cross-sectional areas and depth of invasion in the prognosis of cutaneous melanoma. Ann Surg. 1970 Nov;172(5):902–908. doi: 10.1097/00000658-197011000-00017. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Büchner T., Hiddemann W., Wörmann B., Kleinemeier B., Schumann J., Göhde W., Ritter J., Müller K. M., von Bassewitz D. B., Roessner A. Differential pattern of DNA-aneuploidy in human malignancies. Pathol Res Pract. 1985 Jan;179(3):310–317. doi: 10.1016/s0344-0338(85)80140-0. [DOI] [PubMed] [Google Scholar]
  4. Clark W. H., Jr, From L., Bernardino E. A., Mihm M. C. The histogenesis and biologic behavior of primary human malignant melanomas of the skin. Cancer Res. 1969 Mar;29(3):705–727. [PubMed] [Google Scholar]
  5. Dyson J. E., Joslin C. A., Rothwell R. I., Quirke P., Khoury G. G., Bird C. C. Flow cytofluorometric evidence for the differential radioresponsiveness of aneuploid and diploid cervix tumours. Radiother Oncol. 1987 Mar;8(3):263–272. doi: 10.1016/s0167-8140(87)80249-9. [DOI] [PubMed] [Google Scholar]
  6. Franzén G., Olofsson J., Tytor M., Klintenberg C., Risberg B. Preoperative irradiation in oral cavity carcinoma. A study with special reference to DNA pattern, histological response and prognosis. Acta Oncol. 1987;26(5):349–355. doi: 10.3109/02841868709104359. [DOI] [PubMed] [Google Scholar]
  7. Friedlander M. L., Hedley D. W., Taylor I. W. Clinical and biological significance of aneuploidy in human tumours. J Clin Pathol. 1984 Sep;37(9):961–974. doi: 10.1136/jcp.37.9.961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Goldsmith M. M., Cresson D. S., Postma D. S., Askin F. B., Pillsbury H. C. Significance of ploidy in laryngeal cancer. Am J Surg. 1986 Oct;152(4):396–402. doi: 10.1016/0002-9610(86)90311-9. [DOI] [PubMed] [Google Scholar]
  9. Hansson J., Tribukait B., Lewensohn R., Ringborg U. Flow cytofluorometric DNA analyses of metastases of human malignant melanomas. Anal Quant Cytol. 1982 Jun;4(2):99–104. [PubMed] [Google Scholar]
  10. Jacobsen A. B., Fosså S. D., Lunde S., Melvik J. E., Pettersen E. O. Flow cytometric DNA measurements in paraffin-embedded bladder carcinoma tissue before and after precystectomy radiotherapy. Radiother Oncol. 1987 Oct;10(2):149–155. doi: 10.1016/s0167-8140(87)80057-9. [DOI] [PubMed] [Google Scholar]
  11. Kheir S. M., Bines S. D., Vonroenn J. H., Soong S. J., Urist M. M., Coon J. S. Prognostic significance of DNA aneuploidy in stage I cutaneous melanoma. Ann Surg. 1988 Apr;207(4):455–461. doi: 10.1097/00000658-198804000-00014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kouri M., Laasonen A., Mecklin J. P., Järvinen H., Franssila K., Pyrhönen S. Diploid predominance in hereditary nonpolyposis colorectal carcinoma evaluated by flow cytometry. Cancer. 1990 Apr 15;65(8):1825–1829. doi: 10.1002/1097-0142(19900415)65:8<1825::aid-cncr2820650827>3.0.co;2-h. [DOI] [PubMed] [Google Scholar]
  13. Lindholm C., Hofer P. A., Jonsson H., Tribukait B. Flow DNA-cytometric findings of paraffin-embedded primary cutaneous melanomas related to prognosis. Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;58(2):147–151. doi: 10.1007/BF02890065. [DOI] [PubMed] [Google Scholar]
  14. Look A. T., Hayes F. A., Nitschke R., McWilliams N. B., Green A. A. Cellular DNA content as a predictor of response to chemotherapy in infants with unresectable neuroblastoma. N Engl J Med. 1984 Jul 26;311(4):231–235. doi: 10.1056/NEJM198407263110405. [DOI] [PubMed] [Google Scholar]
  15. Pyrhönen S., Laasonen A., Tammilehto L., Rautonen J., Anttila S., Mattson K., Holsti L. R. Diploid predominance and prognostic significance of S-phase cells in malignant mesothelioma. Eur J Cancer. 1991;27(2):197–200. doi: 10.1016/0277-5379(91)90487-x. [DOI] [PubMed] [Google Scholar]
  16. Seckinger D., Sugarbaker E., Frankfurt O. DNA content in human cancer. Application in pathology and clinical medicine. Arch Pathol Lab Med. 1989 Jun;113(6):619–626. [PubMed] [Google Scholar]
  17. Søndergaard K., Larsen J. K., Møller U., Christensen I. J., Hou-Jensen K. DNA ploidy-characteristics of human malignant melanoma analysed by flow cytometry and compared with histology and clinical course. Virchows Arch B Cell Pathol Incl Mol Pathol. 1983;42(1):43–52. doi: 10.1007/BF02890369. [DOI] [PubMed] [Google Scholar]
  18. Tirindelli-Danesi D., Teodori L., Mauro F., Modini C., Botti C., Cicconetti F., Stipa S. Prognostic significance of flow cytometry in lung cancer. A 5-year study. Cancer. 1987 Aug 15;60(4):844–851. doi: 10.1002/1097-0142(19870815)60:4<844::aid-cncr2820600421>3.0.co;2-3. [DOI] [PubMed] [Google Scholar]
  19. Vindeløv L. L., Christensen I. J., Nissen N. I. A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry. 1983 Mar;3(5):323–327. doi: 10.1002/cyto.990030503. [DOI] [PubMed] [Google Scholar]
  20. Volm M., Mattern J., Sonka J., Vogt-Schaden M., Wayss K. DNA distribution in non-small-cell lung carcinomas and its relationship to clinical behavior. Cytometry. 1985 Jul;6(4):348–356. doi: 10.1002/cyto.990060412. [DOI] [PubMed] [Google Scholar]
  21. Zimmerman P. V., Hawson G. A., Bint M. H., Parsons P. G. Ploidy as a prognostic determinant in surgically treated lung cancer. Lancet. 1987 Sep 5;2(8558):530–533. doi: 10.1016/s0140-6736(87)92923-0. [DOI] [PubMed] [Google Scholar]
  22. von Roenn J. H., Kheir S. M., Wolter J. M., Coon J. S. Significance of DNA abnormalities in primary malignant melanoma and nevi, a retrospective flow cytometric study. Cancer Res. 1986 Jun;46(6):3192–3195. [PubMed] [Google Scholar]

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