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. 1992 Jul;66(1):88–92. doi: 10.1038/bjc.1992.222

Effect of epidermal growth factor in HLA class I and class II transcription and protein expression in human breast adenocarcinoma cell lines.

D J Bernard 1, F Courjal 1, J C Maurizis 1, Y J Bignon 1, P Chollet 1, R Plagne 1
PMCID: PMC1977879  PMID: 1637682

Abstract

The spontaneous expression of HLA class I and class II molecules in two human breast carcinoma cell lines (MCF7, T47D) and their modulation during epidermal growth factor treatment are reported. Transcription was analysed by Northern blot and hybridisation with HLA class II and class I cDNA specific probes. The expression of cell surface determinants was examined by internal protein labelling with 35s-methionine, immunoprecipitation with monoclonal antibodies specific for HLA class I or class II, followed by isolation of the immune complex on protein A-Sepharose; at least a quantification of glycoprotein was performed by chromatofocusing. Glycoprotein quantification showed a significant increase of HLA class I and class II (DR) antigen expression after stimulation by epidermal growth factor (0.02 microgram ml-1) in the two cell lines, when compared with untreated cell controls. However, with epidermal growth factor treatment of MCF7 and T47D cells, low increases in the amounts of HLA class I and class II RNA were obtained. These differences between expressed antigens and correspondent RNA amounts would be explained by the fact that EGF in these two cell lines acts more in post-transcription for HLA class I and class II antigens.

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Selected References

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  1. Auffray C., Korman A. J., Roux-Dosseto M., Bono R., Strominger J. L. cDNA clone for the heavy chain of the human B cell alloantigen DC1: strong sequence homology to the HLA-DR heavy chain. Proc Natl Acad Sci U S A. 1982 Oct;79(20):6337–6341. doi: 10.1073/pnas.79.20.6337. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Auffray C., Rougeon F. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA. Eur J Biochem. 1980 Jun;107(2):303–314. doi: 10.1111/j.1432-1033.1980.tb06030.x. [DOI] [PubMed] [Google Scholar]
  3. Benacerraf B. Antigen processing and presentation. The biologic role of MHC molecules in determinant selection. J Immunol. 1988 Oct 1;141(7 Suppl):S17–S20. [PubMed] [Google Scholar]
  4. Bernard D. J., Maurizis J. C., Chassagne J., Chollet P., Plagne R. Comparison of Class II HLA antigen expression in normal and carcinomatous human breast cells. Cancer Res. 1985 Mar;45(3):1152–1158. [PubMed] [Google Scholar]
  5. Bernard D. J., Maurizis J. C., Chassagne J., Chollet P., Plagne R. Effect of prolactin on class II HLA antigen expression by MCF7 cell line. Anticancer Res. 1986 Jan-Feb;6(1):79–83. [PubMed] [Google Scholar]
  6. Bernard D. J., Maurizis J. C., Chassagne J., Chollet P., Plagne R. N-nitroso-N-methylurea-induced mammary carcinogenesis: effect of prolactin on expression of Ia antigen by tumor cells. J Natl Cancer Inst. 1986 Jun;76(6):1237–1242. [PubMed] [Google Scholar]
  7. Bernard D. J., Maurizis J. C., Chassagne J., Sauvezie B., Fonck Y., Chollet P., Plagne R. Effects of cyclosporine A on Ia antigen expression in N-nitroso-N-methylurea-induced rat mammary tumors. Cancer Res. 1990 Jun 1;50(11):3301–3305. [PubMed] [Google Scholar]
  8. Bernard D. J., Maurizis J. C., Moyret C., Chassagne J., Chollet P., Plagne R. Ovarian hormones, antiestrogen and pregnancy effects on the expression of class II histocompatibility antigens by N-nitroso-N-methylurea-induced rat mammary carcinomas. Immunopharmacology. 1989 May-Jun;17(3):147–155. doi: 10.1016/0162-3109(89)90043-x. [DOI] [PubMed] [Google Scholar]
  9. Bernard D. J., Maurizis J. C., Sauvezie B., Bignon Y. J., Chassagne J., Chollet P., Plagne R. Antagonism of prolactin binding by cyclosporine A on MCF7 breast tumour cell line. Anticancer Res. 1991 Nov-Dec;11(6):2147–2151. [PubMed] [Google Scholar]
  10. Bernard D., Maurizis J. C., Rusé F., Chassagne J., Chollet P., Sauvezie B., de Latour M., Plagne R. Presence of HLA-D/DR antigens on the membrane of breast tumour cells. Clin Exp Immunol. 1984 Apr;56(1):215–221. [PMC free article] [PubMed] [Google Scholar]
  11. Coppin H. L., Denny D. W., Jr, Weissman S. M., McDevitt H. O. HLA-B locus polymorphism: studies with a specific hybridization probe. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8614–8618. doi: 10.1073/pnas.82.24.8614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Fitzpatrick S. L., LaChance M. P., Schultz G. S. Characterization of epidermal growth factor receptor and action on human breast cancer cells in culture. Cancer Res. 1984 Aug;44(8):3442–3447. [PubMed] [Google Scholar]
  13. Haliotis T., Carlow D. A., Elliott B. E. Nonimmunological aspects of MHC function in the regulation of cell proliferation and the malignant phenotype. Cancer Cells. 1990 Mar;2(3):86–90. [PubMed] [Google Scholar]
  14. Houghton A. N., Eisinger M., Albino A. P., Cairncross J. G., Old L. J. Surface antigens of melanocytes and melanomas. Markers of melanocyte differentiation and melanoma subsets. J Exp Med. 1982 Dec 1;156(6):1755–1766. doi: 10.1084/jem.156.6.1755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Howe A. J., Seeger R. C., Molinaro G. A., Ferrone S. Analysis of human tumor cells for Ia-like antigens with monoclonal antibodies. J Natl Cancer Inst. 1981 May;66(5):827–829. [PubMed] [Google Scholar]
  16. Keydar I., Chen L., Karby S., Weiss F. R., Delarea J., Radu M., Chaitcik S., Brenner H. J. Establishment and characterization of a cell line of human breast carcinoma origin. Eur J Cancer. 1979 May;15(5):659–670. doi: 10.1016/0014-2964(79)90139-7. [DOI] [PubMed] [Google Scholar]
  17. Klareskog L., Forsum U., Peterson P. A. Hormonal regulation of the expression of Ia antigens on mammary gland epithelium. Eur J Immunol. 1980 Dec;10(12):958–963. doi: 10.1002/eji.1830101212. [DOI] [PubMed] [Google Scholar]
  18. Lebeau J., Goubin G. Amplification of the epidermal growth factor receptor gene in the BT20 breast carcinoma cell line. Int J Cancer. 1987 Aug 15;40(2):189–191. doi: 10.1002/ijc.2910400210. [DOI] [PubMed] [Google Scholar]
  19. Libermann T. A., Nusbaum H. R., Razon N., Kris R., Lax I., Soreq H., Whittle N., Waterfield M. D., Ullrich A., Schlessinger J. Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin. Nature. 1985 Jan 10;313(5998):144–147. doi: 10.1038/313144a0. [DOI] [PubMed] [Google Scholar]
  20. Merlino G. T., Xu Y. H., Ishii S., Clark A. J., Semba K., Toyoshima K., Yamamoto T., Pastan I. Amplification and enhanced expression of the epidermal growth factor receptor gene in A431 human carcinoma cells. Science. 1984 Apr 27;224(4647):417–419. doi: 10.1126/science.6200934. [DOI] [PubMed] [Google Scholar]
  21. Natali P. G., De Martino C., Quaranta V., Bigotti A., Pellegrino M. A., Ferrone S. Changes in Ia-like antigen expression on malignant human cells. Immunogenetics. 1981;12(3-4):409–413. doi: 10.1007/BF01561680. [DOI] [PubMed] [Google Scholar]
  22. Newman R. A., Ormerod M. G., Greaves M. F. The presence of HLA-DR antigens on lactating human breast epithelium and milk fat globule membranes. Clin Exp Immunol. 1980 Sep;41(3):478–486. [PMC free article] [PubMed] [Google Scholar]
  23. Ng A. K., Pellegrino M. A., Imai K., Ferrone S. HLA-A,B antigens Ia-like antigens, and tumor-associated antigens on prostate carcinoma cell lines: serologic and immunochemical analysis with monoclonal antibodies. J Immunol. 1981 Aug;127(2):443–447. [PubMed] [Google Scholar]
  24. PULVERTAFT J. V. A STUDY OF MALIGNANT TUMOURS IN NIGERIA BY SHORT-TERM TISSUE CULTURE. J Clin Pathol. 1965 May;18:261–273. doi: 10.1136/jcp.18.3.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pfizenmaier K., Bartsch H., Scheurich P., Seliger B., Ucer U., Vehmeyer K., Nagel G. A. Differential gamma-interferon response of human colon carcinoma cells: inhibition of proliferation and modulation of immunogenicity as independent effects of gamma-interferon on tumor cell growth. Cancer Res. 1985 Aug;45(8):3503–3509. [PubMed] [Google Scholar]
  26. Ploegh H. L., Orr H. T., Strominger J. L. Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules. Cell. 1981 May;24(2):287–299. doi: 10.1016/0092-8674(81)90318-4. [DOI] [PubMed] [Google Scholar]
  27. Radka S. F., Charron D. J., Brodsky F. M. Class II molecules of the major histocompatibility complex considered as differentiation markers. Hum Immunol. 1986 Aug;16(4):390–400. doi: 10.1016/0198-8859(86)90065-0. [DOI] [PubMed] [Google Scholar]
  28. Schreiber A. B., Schlessinger J., Edidin M. Interaction between major histocompatibility complex antigens and epidermal growth factor receptors on human cells. J Cell Biol. 1984 Feb;98(2):725–731. doi: 10.1083/jcb.98.2.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shiu R. P. Prolactin receptors in human breast cancer cells in long-term tissue culture. Cancer Res. 1979 Nov;39(11):4381–4386. [PubMed] [Google Scholar]
  30. Soule H. D., Vazguez J., Long A., Albert S., Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst. 1973 Nov;51(5):1409–1416. doi: 10.1093/jnci/51.5.1409. [DOI] [PubMed] [Google Scholar]
  31. Ullrich A., Coussens L., Hayflick J. S., Dull T. J., Gray A., Tam A. W., Lee J., Yarden Y., Libermann T. A., Schlessinger J. Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. 1984 May 31-Jun 6Nature. 309(5967):418–425. doi: 10.1038/309418a0. [DOI] [PubMed] [Google Scholar]
  32. Wake C. T., Long E. O., Strubin M., Gross N., Accolla R., Carrel S., Mach B. Isolation of cDNA clones encoding HLA-DR alpha chains. Proc Natl Acad Sci U S A. 1982 Nov;79(22):6979–6983. doi: 10.1073/pnas.79.22.6979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Yamamoto T., Kamata N., Kawano H., Shimizu S., Kuroki T., Toyoshima K., Rikimaru K., Nomura N., Ishizaki R., Pastan I. High incidence of amplification of the epidermal growth factor receptor gene in human squamous carcinoma cell lines. Cancer Res. 1986 Jan;46(1):414–416. [PubMed] [Google Scholar]
  34. Yunis J. J., Band H., Bonneville F., Yunis E. J. Differential expression of MHC class II antigens in myelomonocytic leukemia cell lines. Blood. 1989 Mar;73(4):931–937. [PubMed] [Google Scholar]
  35. Zinkernagel R. M., Doherty P. C. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness. Adv Immunol. 1979;27:51–177. doi: 10.1016/s0065-2776(08)60262-x. [DOI] [PubMed] [Google Scholar]
  36. de Préval C., Hadam M. R., Mach B. Regulation of genes for HLA class II antigens in cell lines from patients with severe combined immunodeficiency. N Engl J Med. 1988 May 19;318(20):1295–1300. doi: 10.1056/NEJM198805193182003. [DOI] [PubMed] [Google Scholar]

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