Skip to main content
NCBI home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1992 Nov;66(5):794–799. doi: 10.1038/bjc.1992.362

Chemosensitivity testing of fresh human gastric cancer with highly purified tumour cells using the MTT assay.

H Yamaue 1, H Tanimura 1, K Noguchi 1, T Hotta 1, M Tani 1, T Tsunoda 1, M Iwahashi 1, M Tamai 1, S Iwakura 1
PMCID: PMC1977973  PMID: 1419622

Abstract

A major problem associated with the chemosensitivity testing of fresh human tumour cells using the MTT assay is the contamination of nonmalignant cells in the tumour tissues. Highly purified fresh human gastric cancer cells could be obtained from 43 solid tumours and eight malignant ascites for the MTT assay. The success rate of the MTT assay was 87.9% (51 of the 58 cases), and the purity of tumour cells was greater than 90% after separation on Ficoll-Hypaque and Percoll discontinuous gradients in primary, or metastatic lesions, and also ascites. Cisplatin, mitomycin, and doxorubicin were more potent drugs than etoposide and 5-FU against gastric cancer cells. The chemosensitivity in differentiated cancer was equivalent to that in non-differentiated cancer. Twenty of the 51 patients with gastric cancer had evaluable lesions, and they received chemotherapy according to the results of the MTT assay using highly purified tumour cells. A clinical response was obtained in 12 of these 20 patients (response rate: 60.0%; five with complete response, seven with partial response).

Full text

PDF
794

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Campling B. G., Pym J., Baker H. M., Cole S. P., Lam Y. M. Chemosensitivity testing of small cell lung cancer using the MTT assay. Br J Cancer. 1991 Jan;63(1):75–83. doi: 10.1038/bjc.1991.16. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Carmichael J., DeGraff W. G., Gazdar A. F., Minna J. D., Mitchell J. B. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987 Feb 15;47(4):936–942. [PubMed] [Google Scholar]
  3. Hanson J. A., Bentley D. P., Bean E. A., Nute S. R., Moore J. L. In vitro chemosensitivity testing in chronic lymphocytic leukaemia patients. Leuk Res. 1991;15(7):565–569. doi: 10.1016/0145-2126(91)90024-n. [DOI] [PubMed] [Google Scholar]
  4. Kaspers G. J., Pieters R., Van Zantwijk C. H., De Laat P. A., De Waal F. C., Van Wering E. R., Veerman A. J. In vitro drug sensitivity of normal peripheral blood lymphocytes and childhood leukaemic cells from bone marrow and peripheral blood. Br J Cancer. 1991 Sep;64(3):469–474. doi: 10.1038/bjc.1991.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kohnoe S., Moriguchi S., Emi Y., Sakaguchi Y., Maehara Y., Ishida T., Mitsudomi T., Sugimachi K. Lung adenocarcinoma is more sensitive than gastric adenocarcinoma to anticancer drugs in vitro. Eur J Surg Oncol. 1991 Feb;17(1):47–50. [PubMed] [Google Scholar]
  6. Maehara Y., Anai H., Kusumoto H., Sugimachi K. Poorly differentiated human gastric carcinoma is more sensitive to antitumor drugs than is well differentiated carcinoma. Eur J Surg Oncol. 1987 Jun;13(3):203–206. [PubMed] [Google Scholar]
  7. Maehara Y., Kusumoto H., Kusumoto T., Anai H., Sugimachi K. Tumor tissue is more sensitive to mitomycin C, carboquone, and aclacinomycin A than is adjacent normal tissue in vitro. J Surg Oncol. 1989 Jan;40(1):4–7. doi: 10.1002/jso.2930400104. [DOI] [PubMed] [Google Scholar]
  8. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec 16;65(1-2):55–63. doi: 10.1016/0022-1759(83)90303-4. [DOI] [PubMed] [Google Scholar]
  9. Ohyama S., Tanaka M., Yonemura Y., Kinoshita K., Miyazaki I., Sasaki T. In vitro chemosensitivity test of human gastric carcinomas using collagen gel matrix. Jpn J Cancer Res. 1991 May;82(5):607–612. doi: 10.1111/j.1349-7006.1991.tb01893.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Park J. G., Kramer B. S., Steinberg S. M., Carmichael J., Collins J. M., Minna J. D., Gazdar A. F. Chemosensitivity testing of human colorectal carcinoma cell lines using a tetrazolium-based colorimetric assay. Cancer Res. 1987 Nov 15;47(22):5875–5879. [PubMed] [Google Scholar]
  11. Pieters R., Huismans D. R., Leyva A., Veerman A. J. Comparison of the rapid automated MTT-assay with a dye exclusion assay for chemosensitivity testing in childhood leukaemia. Br J Cancer. 1989 Feb;59(2):217–220. doi: 10.1038/bjc.1989.44. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pieters R., Huismans D. R., Loonen A. H., Hählen K., van der Does-van den Berg A., van Wering E. R., Veerman A. J. Relation of cellular drug resistance to long-term clinical outcome in childhood acute lymphoblastic leukaemia. Lancet. 1991 Aug 17;338(8764):399–403. doi: 10.1016/0140-6736(91)91029-t. [DOI] [PubMed] [Google Scholar]
  13. Santini V., Bernabei P. A., Silvestro L., Dal Pozzo O., Bezzini R., Viano I., Gattei V., Saccardi R., Ferrini P. R. In vitro chemosensitivity testing of leukemic cells: prediction of response to chemotherapy in patients with acute non-lymphocytic leukemia. Hematol Oncol. 1989 Jul-Aug;7(4):287–293. doi: 10.1002/hon.2900070405. [DOI] [PubMed] [Google Scholar]
  14. Sargent J. M., Taylor C. G. Appraisal of the MTT assay as a rapid test of chemosensitivity in acute myeloid leukaemia. Br J Cancer. 1989 Aug;60(2):206–210. doi: 10.1038/bjc.1989.252. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Scheithauer W., Clark G. M., Salmon S. E., Dorda W., Shoemaker R. H., Von Hoff D. D. Model for estimation of clinically achievable plasma concentrations for investigational anticancer drugs in man. Cancer Treat Rep. 1986 Dec;70(12):1379–1382. [PubMed] [Google Scholar]
  16. Schroyens W., Tueni E., Dodion P., Bodecker R., Stoessel F., Klastersky J. Validation of clinical predictive value of in vitro colorimetric chemosensitivity assay in head and neck cancer. Eur J Cancer. 1990;26(7):834–838. doi: 10.1016/0277-5379(90)90165-p. [DOI] [PubMed] [Google Scholar]
  17. Suto A. The influence of stromal cells on the MTT assay (I)--In vitro chemosensitivity of the tumor and stromal cells to mitomycin C. Jpn J Surg. 1991 May;21(3):304–307. doi: 10.1007/BF02470951. [DOI] [PubMed] [Google Scholar]
  18. Twentyman P. R., Fox N. E., Rees J. K. Chemosensitivity testing of fresh leukaemia cells using the MTT colorimetric assay. Br J Haematol. 1989 Jan;71(1):19–24. doi: 10.1111/j.1365-2141.1989.tb06268.x. [DOI] [PubMed] [Google Scholar]
  19. Twentyman P. R., Luscombe M. A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. Br J Cancer. 1987 Sep;56(3):279–285. doi: 10.1038/bjc.1987.190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weisenthal L. M., Dill P. L., Finklestein J. Z., Duarte T. E., Baker J. A., Moran E. M. Laboratory detection of primary and acquired drug resistance in human lymphatic neoplasms. Cancer Treat Rep. 1986 Nov;70(11):1283–1295. [PubMed] [Google Scholar]
  21. Wilson J. K., Sargent J. M., Elgie A. W., Hill J. G., Taylor C. G. A feasibility study of the MTT assay for chemosensitivity testing in ovarian malignancy. Br J Cancer. 1990 Aug;62(2):189–194. doi: 10.1038/bjc.1990.258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Yamauchi M., Satta T., Ito A., Kondo T., Takagi H. A feasibility study of the SDI test for the evaluation of gastrointestinal cancer sensitivity to anticancer drugs. J Surg Oncol. 1991 Aug;47(4):253–260. doi: 10.1002/jso.2930470410. [DOI] [PubMed] [Google Scholar]
  23. Yamaue H., Tanimura H., Terashita S., Iwahashi M., Tani M., Tsunoda T., Tamai M., Mori K. Clinical evaluation of chemotherapy under angiotensin II-induced hypertension in patients with advanced cancer. Nihon Geka Hokan. 1990 Jul 1;59(4):302–309. [PubMed] [Google Scholar]
  24. Yamaue H., Tanimura H., Tsunoda T., Iwahashi M., Tani M., Tamai M., Inoue M. Functional and phenotypic analyses of interleukin 2-activated tumor-infiltrating lymphocytes. Biotherapy. 1990;2(3):247–259. doi: 10.1007/BF02173526. [DOI] [PubMed] [Google Scholar]
  25. Yamaue H., Tanimura H., Tsunoda T., Tani M., Iwahashi M., Noguchi K., Tamai M., Hotta T., Arii K. Chemosensitivity testing with highly purified fresh human tumour cells with the MTT colorimetric assay. Eur J Cancer. 1991;27(10):1258–1263. doi: 10.1016/0277-5379(91)90093-s. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES