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. 1992 Nov;66(5):925–929. doi: 10.1038/bjc.1992.387

Lack of prognostic significance of DNA ploidy and S phase fraction in breast cancer.

P D Stanton 1, T G Cooke 1, S J Oakes 1, J Winstanley 1, S Holt 1, W D George 1, G D Murray 1
PMCID: PMC1977991  PMID: 1419638

Abstract

DNA Ploidy and S phase fraction (SPF) were measured in Stage I and II breast cancers from patients with at least 8 years of follow-up, to assess the prognostic significance of these data. Disaggregated sections of formalin-fixed, paraffin-embedded tumour were analysed by flow cytometry. SPF was calculated using a rectangular model of S phase, after subtraction of background debris using an exponential model. 64% of tumours were DNA aneuploid. The median SPF was 4.5% for DNA diploid, and 10.9% for DNA aneuploid tumours. There were small reductions in survival at 10 years for DNA aneuploid tumours, and for tumours with above median SPF, but these were not statistically significant. The relative hazard for DNA aneuploid tumours was 1.20 (95% CI 0.81-1.76), and for high SPF was 1.31 (95% CI 0.87-1.98). Neither factor was statistically correlated with survival in multivariate analysis. Technical and theoretical factors limit the accuracy and reproducibility of flow cytometric data, and may explain the lack of prognostic information given.

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Selected References

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  1. Clark G. M., Dressler L. G., Owens M. A., Pounds G., Oldaker T., McGuire W. L. Prediction of relapse or survival in patients with node-negative breast cancer by DNA flow cytometry. N Engl J Med. 1989 Mar 9;320(10):627–633. doi: 10.1056/NEJM198903093201003. [DOI] [PubMed] [Google Scholar]
  2. Cornelisse C. J., van de Velde C. J., Caspers R. J., Moolenaar A. J., Hermans J. DNA ploidy and survival in breast cancer patients. Cytometry. 1987 Mar;8(2):225–234. doi: 10.1002/cyto.990080217. [DOI] [PubMed] [Google Scholar]
  3. Fisher B., Gunduz N., Costantino J., Fisher E. R., Redmond C., Mamounas E. P., Siderits R. DNA flow cytometric analysis of primary operable breast cancer. Relation of ploidy and S-phase fraction to outcome of patients in NSABP B-04. Cancer. 1991 Oct 1;68(7):1465–1475. doi: 10.1002/1097-0142(19911001)68:7<1465::aid-cncr2820680702>3.0.co;2-i. [DOI] [PubMed] [Google Scholar]
  4. Frierson H. F., Jr Ploidy analysis and S-phase fraction determination by flow cytometry of invasive adenocarcinomas of the breast. Am J Surg Pathol. 1991 Apr;15(4):358–367. doi: 10.1097/00000478-199104000-00004. [DOI] [PubMed] [Google Scholar]
  5. Fuhr J. E., Frye A., Kattine A. A., Van Meter S. Flow cytometric determination of breast tumor heterogeneity. Cancer. 1991 Mar 1;67(5):1401–1405. doi: 10.1002/1097-0142(19910301)67:5<1401::aid-cncr2820670521>3.0.co;2-4. [DOI] [PubMed] [Google Scholar]
  6. Hedley D. W., Friedlander M. L., Taylor I. W. Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance. Cytometry. 1985 Jul;6(4):327–333. doi: 10.1002/cyto.990060409. [DOI] [PubMed] [Google Scholar]
  7. Hedley D. W., Friedlander M. L., Taylor I. W., Rugg C. A., Musgrove E. A. Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem. 1983 Nov;31(11):1333–1335. doi: 10.1177/31.11.6619538. [DOI] [PubMed] [Google Scholar]
  8. Hedley D. W., Rugg C. A., Gelber R. D. Association of DNA index and S-phase fraction with prognosis of nodes positive early breast cancer. Cancer Res. 1987 Sep 1;47(17):4729–4735. [PubMed] [Google Scholar]
  9. Kallioniemi O. P., Blanco G., Alavaikko M., Hietanen T., Mattila J., Lauslahti K., Lehtinen M., Koivula T. Improving the prognostic value of DNA flow cytometry in breast cancer by combining DNA index and S-phase fraction. A proposed classification of DNA histograms in breast cancer. Cancer. 1988 Nov 15;62(10):2183–2190. doi: 10.1002/1097-0142(19881115)62:10<2183::aid-cncr2820621019>3.0.co;2-b. [DOI] [PubMed] [Google Scholar]
  10. Kallioniemi O. P. Comparison of fresh and paraffin-embedded tissue as starting material for DNA flow cytometry and evaluation of intratumor heterogeneity. Cytometry. 1988 Mar;9(2):164–169. doi: 10.1002/cyto.990090211. [DOI] [PubMed] [Google Scholar]
  11. Merkel D. E., McGuire W. L. Ploidy, proliferative activity and prognosis. DNA flow cytometry of solid tumors. Cancer. 1990 Mar 1;65(5):1194–1205. doi: 10.1002/1097-0142(19900301)65:5<1194::aid-cncr2820650528>3.0.co;2-m. [DOI] [PubMed] [Google Scholar]
  12. Stål O., Wingren S., Carstensen J., Rutqvist L. E., Skoog L., Klintenberg C., Nordenskjöld B. Prognostic value of DNA ploidy and S-phase fraction in relation to estrogen receptor content and clinicopathological variables in primary breast cancer. Eur J Cancer Clin Oncol. 1989 Feb;25(2):301–309. doi: 10.1016/0277-5379(89)90023-0. [DOI] [PubMed] [Google Scholar]
  13. Toikkanen S., Joensuu H., Klemi P. The prognostic significance of nuclear DNA content in invasive breast cancer--a study with long-term follow-up. Br J Cancer. 1989 Nov;60(5):693–700. doi: 10.1038/bjc.1989.342. [DOI] [PMC free article] [PubMed] [Google Scholar]

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