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. 1992 Nov;66(5):893–899. doi: 10.1038/bjc.1992.381

Prior chemotherapy does not prevent effective mobilisation by G-CSF of peripheral blood progenitor cells.

E DeLuca 1, W P Sheridan 1, D Watson 1, J Szer 1, C G Begley 1
PMCID: PMC1977995  PMID: 1384644

Abstract

In this study we demonstrate that the hemopoietic growth factor, G-CSF successfully mobilised progenitor cell populations into the peripheral blood in a population of patients despite intensive pretreatment with chemotherapy. Administration of G-CSF increased the numbers of peripheral blood progenitor cells (PBPC) by a median of 76-fold above basal levels. Maximal levels of PBPC were observed on days 5 and 6 after G-CSF treatment. In two patients a second cycle of G-CSF mobilised PBPC to levels comparable with those seen after the first cycle of G-CSF treatment. An earlier hemopoietic cell population (pre-CFC's) was also mobilised with levels increased up to 50-fold above basal levels. Using a standard mononuclear cell leukapheresis technique the PBPC were collected extremely efficiently (essentially 100%) and could be further successfully enriched by separation using a Ficoll gradient. For patients who underwent the optimal collection protocol (i.e. leukapheresis on days 5, 6 and 7) a total of 32 +/- 6 x 10(4) GM-CFC kg-1 were collected. The ability to mobilise PBPC using G-CSF alone and to successfully and efficiently harvest these cells has important implications for the future of transplantation and high dose chemotherapy procedures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews R. G., Knitter G. H., Bartelmez S. H., Langley K. E., Farrar D., Hendren R. W., Appelbaum F. R., Bernstein I. D., Zsebo K. M. Recombinant human stem cell factor, a c-kit ligand, stimulates hematopoiesis in primates. Blood. 1991 Oct 15;78(8):1975–1980. [PubMed] [Google Scholar]
  2. Barr R. D., McBride J. A. Haemopoietic engraftment with peripheral blood cells in the treatment of malignant disease. Br J Haematol. 1982 Jun;51(2):181–187. [PubMed] [Google Scholar]
  3. Dührsen U., Villeval J. L., Boyd J., Kannourakis G., Morstyn G., Metcalf D. Effects of recombinant human granulocyte colony-stimulating factor on hematopoietic progenitor cells in cancer patients. Blood. 1988 Dec;72(6):2074–2081. [PubMed] [Google Scholar]
  4. Gabrilove J. L., Jakubowski A., Fain K., Grous J., Scher H., Sternberg C., Yagoda A., Clarkson B., Bonilla M. A., Oettgen H. F. Phase I study of granulocyte colony-stimulating factor in patients with transitional cell carcinoma of the urothelium. J Clin Invest. 1988 Oct;82(4):1454–1461. doi: 10.1172/JCI113751. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gianni A. M., Siena S., Bregni M., Tarella C., Stern A. C., Pileri A., Bonadonna G. Granulocyte-macrophage colony-stimulating factor to harvest circulating haemopoietic stem cells for autotransplantation. Lancet. 1989 Sep 9;2(8663):580–585. doi: 10.1016/s0140-6736(89)90711-3. [DOI] [PubMed] [Google Scholar]
  6. Haas R., Ho A. D., Bredthauer U., Cayeux S., Egerer G., Knauf W., Hunstein W. Successful autologous transplantation of blood stem cells mobilized with recombinant human granulocyte-macrophage colony-stimulating factor. Exp Hematol. 1990 Feb;18(2):94–98. [PubMed] [Google Scholar]
  7. Jacobsen N., Broxmeyer H. E., Grossbard E., Moore M. A. Colony-forming units in diffusion chambers (CFU-d) and colony-forming units in agar culture (CFU-c) obtained from normal human bone marrow: a possible parent-progeny relationship. Cell Tissue Kinet. 1979 Mar;12(2):213–226. doi: 10.1111/j.1365-2184.1979.tb00127.x. [DOI] [PubMed] [Google Scholar]
  8. Metcalf D., Nicola N. A. Proliferative effects of purified granulocyte colony-stimulating factor (G-CSF) on normal mouse hemopoietic cells. J Cell Physiol. 1983 Aug;116(2):198–206. doi: 10.1002/jcp.1041160211. [DOI] [PubMed] [Google Scholar]
  9. Moore M. A., Broxmeyer H. E., Sheridan A. P., Meyers P. A., Jacobsen N., Winchester R. J. Continuous human bone marrow culture: Ia antigen characterization of probable pluripotential stem cells. Blood. 1980 Apr;55(4):682–690. [PubMed] [Google Scholar]
  10. Nagata S., Tsuchiya M., Asano S., Kaziro Y., Yamazaki T., Yamamoto O., Hirata Y., Kubota N., Oheda M., Nomura H. Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor. 1986 Jan 30-Feb 5Nature. 319(6052):415–418. doi: 10.1038/319415a0. [DOI] [PubMed] [Google Scholar]
  11. Nicola N. A., Johnson G. R. The production of committed hemopoietic colony-forming cells from multipotential precursor cells in vitro. Blood. 1982 Oct;60(4):1019–1029. [PubMed] [Google Scholar]
  12. Nicola N. A., Metcalf D., Johnson G. R., Burgess A. W. Separation of functionally distinct human granulocyte-macrophage colony-stimulating factors. Blood. 1979 Sep;54(3):614–627. [PubMed] [Google Scholar]
  13. Ottmann O. G., Ganser A., Seipelt G., Eder M., Schulz G., Hoelzer D. Effects of recombinant human interleukin-3 on human hematopoietic progenitor and precursor cells in vivo. Blood. 1990 Oct 15;76(8):1494–1502. [PubMed] [Google Scholar]
  14. Richman C. M., Weiner R. S., Yankee R. A. Increase in circulating stem cells following chemotherapy in man. Blood. 1976 Jun;47(6):1031–1039. [PubMed] [Google Scholar]
  15. Sheridan W. P., Begley C. G., Juttner C. A., Szer J., To L. B., Maher D., McGrath K. M., Morstyn G., Fox R. M. Effect of peripheral-blood progenitor cells mobilised by filgrastim (G-CSF) on platelet recovery after high-dose chemotherapy. Lancet. 1992 Mar 14;339(8794):640–644. doi: 10.1016/0140-6736(92)90795-5. [DOI] [PubMed] [Google Scholar]
  16. Sheridan W. P., Morstyn G., Wolf M., Dodds A., Lusk J., Maher D., Layton J. E., Green M. D., Souza L., Fox R. M. Granulocyte colony-stimulating factor and neutrophil recovery after high-dose chemotherapy and autologous bone marrow transplantation. Lancet. 1989 Oct 14;2(8668):891–895. doi: 10.1016/s0140-6736(89)91552-3. [DOI] [PubMed] [Google Scholar]
  17. Socinski M. A., Cannistra S. A., Elias A., Antman K. H., Schnipper L., Griffin J. D. Granulocyte-macrophage colony stimulating factor expands the circulating haemopoietic progenitor cell compartment in man. Lancet. 1988 May 28;1(8596):1194–1198. doi: 10.1016/s0140-6736(88)92012-0. [DOI] [PubMed] [Google Scholar]
  18. To L. B., Dyson P. G., Juttner C. A. Cell-dose effect in circulating stem-cell autografting. Lancet. 1986 Aug 16;2(8503):404–405. doi: 10.1016/s0140-6736(86)90096-6. [DOI] [PubMed] [Google Scholar]
  19. To L. B., Haylock D. N., Kimber R. J., Juttner C. A. High levels of circulating haemopoietic stem cells in very early remission from acute non-lymphoblastic leukaemia and their collection and cryopreservation. Br J Haematol. 1984 Nov;58(3):399–410. doi: 10.1111/j.1365-2141.1984.tb03987.x. [DOI] [PubMed] [Google Scholar]
  20. To L. B., Shepperd K. M., Haylock D. N., Dyson P. G., Charles P., Thorp D. L., Dale B. M., Dart G. W., Roberts M. M., Sage R. E. Single high doses of cyclophosphamide enable the collection of high numbers of hemopoietic stem cells from the peripheral blood. Exp Hematol. 1990 Jun;18(5):442–447. [PubMed] [Google Scholar]
  21. Ulich T. R., del Castillo J., McNiece I. K., Yi E. S., Alzona C. P., Yin S. M., Zsebo K. M. Stem cell factor in combination with granulocyte colony-stimulating factor (CSF) or granulocyte-macrophage CSF synergistically increases granulopoiesis in vivo. Blood. 1991 Oct 15;78(8):1954–1962. [PubMed] [Google Scholar]
  22. Villeval J. L., Dührsen U., Morstyn G., Metcalf D. Effect of recombinant human granulocyte-macrophage colony stimulating factor on progenitor cells in patients with advanced malignancies. Br J Haematol. 1990 Jan;74(1):36–44. doi: 10.1111/j.1365-2141.1990.tb02535.x. [DOI] [PubMed] [Google Scholar]
  23. Zsebo K. M., Williams D. A., Geissler E. N., Broudy V. C., Martin F. H., Atkins H. L., Hsu R. Y., Birkett N. C., Okino K. H., Murdock D. C. Stem cell factor is encoded at the Sl locus of the mouse and is the ligand for the c-kit tyrosine kinase receptor. Cell. 1990 Oct 5;63(1):213–224. doi: 10.1016/0092-8674(90)90302-u. [DOI] [PubMed] [Google Scholar]

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