Conserved elements of PilT fold. (A) The NTD of PilT (colored as in Fig. 2A) resembles the well-known PAS domain (grey, represented by the circadian clock protein Period (Yildiz et al., 2005)). Non-canonical PAS elements (PilT αA and loops within Period) are removed for clarity. (B) The core ATPase subdomain of PilT (green) is readily superimposable upon RecA (Story and Steitz, 1992) (grey). In this view the least squares calculation is over P-loop residues only. Type II/IV secretion ATPase family motifs Walker A (blue), Asp Box (lime green), Walker B (magenta) and His Box (orange) neighbor the bound nucleotide. (C) Isolated, magnified view of the four sequence motifs described in (B), with ADP and signature invariant residues Lys149, Glu176, Glu217 and His242 depicted (blue, green, magenta, and orange, respectively).