FIGURE 6.

THG73 acceptor stem mutations tested at β9′UAG. Individual tRNA (74 mer) (16 ng per oocyte) average current was normalized to THG73 (74 mer) average current. Bar colors are the same as in Fig. 5. Total of oocytes tested is 189 and each bar is 26>n>8 oocytes. All mutations in the acceptor stem lower aminoacylation in vivo relative to THG73. TQAS-1 shows lack of aminoacylation but is not accepted by the translational machinery (Rodriguez et al. 2007, companion paper, this issue). TQAS′ and TQAS are the most orthogonal tRNAs in Xenopus Express and Nasco oocytes, respectively.