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tRNA aminoacylation tested at αK145. Total of oocytes tested is 36, where each dose–response is 17>n>5 oocytes. EC50 values are 167 ± 14, 165 ± 14, 134 ± 15, and 144 ± 2 μM ACh in the order listed in the legend, with 7.5 ng of tRNA per oocyte. All tRNAs are still aminoacylated by Gln and not Lys (wild-type EC50 = 50 μM ACh).
