Skip to main content
PMC home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1984 Feb;81(2):301–307. doi: 10.1111/j.1476-5381.1984.tb10079.x

Endothelium-dependent relaxation induced by angiotensin II and histamine in isolated arteries of dog.

N Toda
PMCID: PMC1986885  PMID: 6367872

Abstract

In helical strips of dog renal and mesenteric arteries pre-contracted with prostaglandin F2 alpha (PGF2 alpha), endothelium-dependent relaxations were investigated. Removal of the endothelium was shown histologically by staining with silver nitrate and functionally by testing the inability of acetylcholine to induce arterial relaxations. When the endothelium was removed, relaxation of renal arteries to angiotensin (Ang) II was markedly suppressed, whereas relaxations induced by PGI2 or isoprenaline were attenuated only slightly. Removal of the endothelium attenuated the relaxant response of mesenteric arteries to histamine but did not significantly alter the response to PGI2. Treatment with indomethacin caused an additional attenuation of the relaxant response to histamine or a reversal of the Ang II-induced relaxation to a contraction in the arterial strips, from which the endothelium had been removed. Relaxation of renal arteries induced by Ang II and of mesenteric arteries induced by histamine is postulated to result from PGI2 released from the arterial wall. Therefore, it appears that the endothelium is a major site but not the only site responsible for drug-induced release of PGI2.

Full text

PDF
301

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baenziger N. L., Force L. E., Becherer P. R. Histamine stimulate prostacyclin synthesis in cultured human umbilical vein endothelial cells. Biochem Biophys Res Commun. 1980 Feb 27;92(4):1435–1440. doi: 10.1016/0006-291x(80)90447-7. [DOI] [PubMed] [Google Scholar]
  2. Caplan B. A., Gerrity R. G., Schwartz C. J. Endothelial cell morphology in focal areas of in vivo Evans blue uptake in the young pig aorta. I. Quantitative light microscopic findings. Exp Mol Pathol. 1974 Aug;21(1):102–117. doi: 10.1016/0014-4800(74)90082-3. [DOI] [PubMed] [Google Scholar]
  3. Desjardins-Giasson S., Gutkowska J., Garcia R., Genest J. Effect of angiotensin ii and norepinephrine on release of prostaglandins E2 and I2 by the perfused rat mesenteric artery. Prostaglandins. 1982 Jul;24(1):105–114. doi: 10.1016/0090-6980(82)90182-4. [DOI] [PubMed] [Google Scholar]
  4. Dusting G. J. Angiotensin-induced release of a prostacyclin-like substance from the lungs. J Cardiovasc Pharmacol. 1981 Jan-Feb;3(1):197–206. doi: 10.1097/00005344-198101000-00018. [DOI] [PubMed] [Google Scholar]
  5. Eldor A., Falcone D. J., Hajjar D. P., Minick C. R., Weksler B. B. Recovery of prostacyclin production by de-endothelialized rabbit aorta. Critical role of neointimal smooth muscle cells. J Clin Invest. 1981 Mar;67(3):735–741. doi: 10.1172/JCI110090. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Furchgott R. F., Zawadzki J. V. The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature. 1980 Nov 27;288(5789):373–376. doi: 10.1038/288373a0. [DOI] [PubMed] [Google Scholar]
  7. Gimbrone M. A., Jr, Alexander R. W. Angiotensin II stimulation of prostaglandin production in cultured human vascular endothelium. Science. 1975 Jul 18;189(4198):219–220. doi: 10.1126/science.1138377. [DOI] [PubMed] [Google Scholar]
  8. Goldsmith J. C. Contribution of the subendothelium to prostacyclin release after vascular injury. J Lab Clin Med. 1982 Oct;100(4):574–584. [PubMed] [Google Scholar]
  9. Konishi M., Toda N., Yamamoto M. Different mechanisms of action of histamine in isolated arteries of the dog. Br J Pharmacol. 1981 Sep;74(1):111–118. doi: 10.1111/j.1476-5381.1981.tb09961.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Marcus A. J., Weksler B. B., Jaffe E. A. Enzymatic conversion of prostaglandin endoperoxide H2 and arachidonic acid to prostacyclin by cultured human endothelial cells. J Biol Chem. 1978 Oct 25;253(20):7138–7141. [PubMed] [Google Scholar]
  11. Moncada S., Herman A. G., Higgs E. A., Vane J. R. Differential formation of prostacyclin (PGX or PGI2) by layers of the arterial wall. An explanation for the anti-thrombotic properties of vascular endothelium. Thromb Res. 1977 Sep;11(3):323–344. doi: 10.1016/0049-3848(77)90185-2. [DOI] [PubMed] [Google Scholar]
  12. Nolan R. D., Dusting G. J., Martin T. J. Phospholipase inhibition and the mechanism of angiotensin-induced prostacyclin release from rat mesenteric vasculature. Biochem Pharmacol. 1981 Aug 1;30(15):2121–2125. doi: 10.1016/0006-2952(81)90231-8. [DOI] [PubMed] [Google Scholar]
  13. Shebuski R. J., Aiken J. W. Angiotensin II stimulation of renal prostaglandin synthesis elevates circulating prostacyclin in the dog. J Cardiovasc Pharmacol. 1980 Sep-Oct;2(5):667–677. doi: 10.1097/00005344-198009000-00015. [DOI] [PubMed] [Google Scholar]
  14. Shibouta Y., Inada Y., Terashita Z., Nishikawa K., Kikuchi S., Shimamoto K. Angiotensin-II-stimulated release of thromboxane A2 and prostacyclin (PGI2) in isolated, perfused kidneys of spontaneously hypertensive rats. Biochem Pharmacol. 1979 Dec 15;28(24):3601–3609. doi: 10.1016/0006-2952(79)90406-4. [DOI] [PubMed] [Google Scholar]
  15. Toda N., Hatano Y., Hayashi S. Modifications by stretches of the mechanical response of isolated cerebral and extracerebral arteries to vasoactive agents. Pflugers Arch. 1978 Apr 25;374(1):73–77. doi: 10.1007/BF00585699. [DOI] [PubMed] [Google Scholar]
  16. Toda N., Konishi M., Miyazaki M. Involvement of endogenous prostaglandin I2 in the vascular action of histamine in dogs. J Pharmacol Exp Ther. 1982 Oct;223(1):257–262. [PubMed] [Google Scholar]
  17. Toda N., Miyazaki M. Angiotensin-induced relaxation in isolated dog renal and cerebral arteries. Am J Physiol. 1981 Feb;240(2):H247–H254. doi: 10.1152/ajpheart.1981.240.2.H247. [DOI] [PubMed] [Google Scholar]
  18. Toda N. The action of vasodilating drugs on isolated basilar, coronary and mesenteric arteries of the dog. J Pharmacol Exp Ther. 1974 Oct;191(1):139–146. [PubMed] [Google Scholar]
  19. Van de Voorde J., Leusen I. Role of the endothelium in the vasodilator response of rat thoracic aorta to histamine. Eur J Pharmacol. 1983 Jan 28;87(1):113–120. doi: 10.1016/0014-2999(83)90056-0. [DOI] [PubMed] [Google Scholar]
  20. Weksler B. B., Ley C. W., Jaffe E. A. Stimulation of endothelial cell prostacyclin production by thrombin, trypsin, and the ionophore A 23187. J Clin Invest. 1978 Nov;62(5):923–930. doi: 10.1172/JCI109220. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES