Skip to main content
NCBI home page
British Journal of Pharmacology logoLink to British Journal of Pharmacology
. 1984 Jan;81(1):169–174. doi: 10.1111/j.1476-5381.1984.tb10757.x

The regulation of delta-opiate receptor density on 108CC15 neuroblastoma X glioma hybrid cells.

M A Moses, C R Snell
PMCID: PMC1986939  PMID: 6322893

Abstract

The effect of exogenous substances on the expression of opiate receptors on 108CC15 neuroblastoma X glioma hybrid cells has been studied. Cell differentiation by culture in the presence of N6-O2-dibutyryl adenosine 3',5'-cyclic monophosphate induced a three fold increase in opiate receptor density. When the cells were grown in the presence of 10(-5) M morphine hydrochloride for up to 23 days, opiate receptor densities were reduced by only 30% when compared with matched controls. Culture in the presence of 10(-7) M D-Ala2-D-Leu5-enkephalin produced opiate receptor down regulation of 73% compared to controls after only 4 h of treatment. The down regulation process could be inhibited by continued exposure to D-Ala2 D-Leu5-enkephalin at concentrations greater than 4 nM; below this concentration down regulation was rapid and irreversible. A model to explain these observations is described.

Full text

PDF
169

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amsterdam A., Berkowitz A., Nimrod A., Kohen F. Aggregation of luteinizing hormone receptors in granulosa cells: a possible mechanism of desensitization to the hormone. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3440–3444. doi: 10.1073/pnas.77.6.3440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blanchard S. G., Chang K. J., Cuatrecasas P. Characterization of the association of tritiated enkephalin with neuroblastoma cells under conditions optimal for receptor down regulation. J Biol Chem. 1983 Jan 25;258(2):1092–1097. [PubMed] [Google Scholar]
  3. Brandt M., Fischer K., Moroder L., Wunsch E., Hamprecht B. Enkephalin evokes biochemical correlates of opiate tolerance and dependence in neuroblastoma x glioma hybrid cells. FEBS Lett. 1976 Sep 15;68(1):38–40. doi: 10.1016/0014-5793(76)80399-7. [DOI] [PubMed] [Google Scholar]
  4. Burt D. R., Snyder S. H. Thyrotropin releasing hormone (TRH): apparent receptor binding in rat brain membranes. Brain Res. 1975 Aug 8;93(2):309–328. doi: 10.1016/0006-8993(75)90353-4. [DOI] [PubMed] [Google Scholar]
  5. Carpenter G., Cohen S. 125I-labeled human epidermal growth factor. Binding, internalization, and degradation in human fibroblasts. J Cell Biol. 1976 Oct;71(1):159–171. doi: 10.1083/jcb.71.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chang K. J., Cuatrecasas P. Multiple opiate receptors. Enkephalins and morphine bind to receptors of different specificity. J Biol Chem. 1979 Apr 25;254(8):2610–2618. [PubMed] [Google Scholar]
  7. Chang K. J., Miller R. J., Cuatrecasas P. Interaction of enkephalin with opiate receptors in intact cultured cells. Mol Pharmacol. 1978 Nov;14(6):961–970. [PubMed] [Google Scholar]
  8. Clendeninn N. J., Petraitis M., Simon E. J. Ontological development of opiate receptors in rodent brain. Brain Res. 1976 Dec 10;118(1):157–160. doi: 10.1016/0006-8993(76)90852-0. [DOI] [PubMed] [Google Scholar]
  9. Haigler H., Ash J. F., Singer S. J., Cohen S. Visualization by fluorescence of the binding and internalization of epidermal growth factor in human carcinoma cells A-431. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3317–3321. doi: 10.1073/pnas.75.7.3317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hamprecht B. Structural, electrophysiological, biochemical, and pharmacological properties of neuroblastoma-glioma cell hybrids in cell culture. Int Rev Cytol. 1977;49:99–170. doi: 10.1016/s0074-7696(08)61948-8. [DOI] [PubMed] [Google Scholar]
  11. Hazum E., Chang K. J., Cuatrecasas P. Opiate (Enkephalin) receptors of neuroblastoma cells: occurrence in clusters on the cell surface. Science. 1979 Nov 30;206(4422):1077–1079. doi: 10.1126/science.227058. [DOI] [PubMed] [Google Scholar]
  12. Hazum E., Cuatrecasas P., Marian J., Conn P. M. Receptor-mediated internalization of fluorescent gonadotropin-releasing hormone by pituitary gonadotropes. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6692–6695. doi: 10.1073/pnas.77.11.6692. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Johnson E. M., Jr, Andres R. Y., Bradshaw R. A. Characterization of the retrograde transport of nerve growth factor (NGF) using high specific activity [125I] NGF. Brain Res. 1978 Jul 14;150(2):319–331. doi: 10.1016/0006-8993(78)90283-4. [DOI] [PubMed] [Google Scholar]
  14. Klee W. A., Nirenberg M. A neuroblastoma times glioma hybrid cell line with morphine receptors. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3474–3477. doi: 10.1073/pnas.71.9.3474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  16. Lampert A., Nirenberg M., Klee W. A. Tolerance and dependence evoked by an endogenous opiate peptide. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3165–3167. doi: 10.1073/pnas.73.9.3165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mukherjee C., Caron M. G., Lefkowitz R. J. Catecholamine-induced subsensitivity of adenylate cyclase associated with loss of beta-adrenergic receptor binding sites. Proc Natl Acad Sci U S A. 1975 May;72(5):1945–1949. doi: 10.1073/pnas.72.5.1945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schlessinger J., Shechter Y., Willingham M. C., Pastan I. Direct visualization of binding, aggregation, and internalization of insulin and epidermal growth factor on living fibroblastic cells. Proc Natl Acad Sci U S A. 1978 Jun;75(6):2659–2663. doi: 10.1073/pnas.75.6.2659. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Schlessinger J., Van Obberghen E., Kahn C. R. Insulin and antibodies against insulin receptor cap on the membrane of cultured human lymphocytes. Nature. 1980 Aug 14;286(5774):729–731. doi: 10.1038/286729a0. [DOI] [PubMed] [Google Scholar]
  20. Sharma S. K., Klee W. A., Nirenberg M. Dual regulation of adenylate cyclase accounts for narcotic dependence and tolerance. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3092–3096. doi: 10.1073/pnas.72.8.3092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sharma S. K., Nirenberg M., Klee W. A. Morphine receptors as regulators of adenylate cyclase activity. Proc Natl Acad Sci U S A. 1975 Feb;72(2):590–594. doi: 10.1073/pnas.72.2.590. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Su Y. F., Harden T. K., Perkins J. P. Catecholamine-specific desensitization of adenylate cyclase. Evidence for a multistep process. J Biol Chem. 1980 Aug 10;255(15):7410–7419. [PubMed] [Google Scholar]
  23. Su Y. F., Harden T. K., Perkins J. P. Isoproterenol-induced desensitization of adenylate cyclase in human astrocytoma cells. Relation of loss of hormonal responsiveness and decrement in beta-adrenergic receptors. J Biol Chem. 1979 Jan 10;254(1):38–41. [PubMed] [Google Scholar]
  24. Traber J., Fischer K., Latzin S., Hamprecht B. Morphine antagonises action of prostaglandin in neuroblastoma and neuroblastoma times glioma hybrid cells. Nature. 1975 Jan 10;253(5487):120–122. doi: 10.1038/253120a0. [DOI] [PubMed] [Google Scholar]
  25. Wahlström A., Brandt M., Moroder L., Wünsch E., Lindeberg G., Ragnarsson U., Terenius L., Hamprecht B. Peptides related to beta-lipotropin with opioid activity. Effects on levels of adenosine 3':5'-cyclic monophosphate in neuroblastoma x glioma hybrid cells. FEBS Lett. 1977 May 1;77(1):28–32. doi: 10.1016/0014-5793(77)80186-5. [DOI] [PubMed] [Google Scholar]
  26. Wessels M. R., Mullikin D., Lefkowitz R. J. Differences between agonist and antagonist binding following beta-adrenergic receptor desensitization. J Biol Chem. 1978 May 25;253(10):3371–3373. [PubMed] [Google Scholar]

Articles from British Journal of Pharmacology are provided here courtesy of The British Pharmacological Society

RESOURCES