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. 1984 Jul;82(3):673–677. doi: 10.1111/j.1476-5381.1984.tb10806.x

Effects of neomycin on galactose absorption across rat jejunum.

E S Debnam, C S Thompson
PMCID: PMC1987012  PMID: 6743918

Abstract

The effects of neomycin sulphate on galactose absorption have been studied using in vivo and in vitro preparations of rat small intestine. Neomycin (10(-3)M) produced an increase in the maximum transport capacity (Jmax) for the active component of absorption in vivo. The apparent Kt for absorption was unaffected. The antibiotic caused a dose-dependent increase in the potential difference across the mucosal membrane (Vm) measured in vitro, a maximal effect being seen at a concentration of 10(-4)M. Furthermore, the magnitude of the depolarization induced by the addition of galactose (4 mM) to the mucosal fluid was enhanced by neomycin (10(-4)M). Phlorhizin (10(-4)M) abolished the galactose-induced depolarization in both the absence and presence of the antibiotic. It is concluded that neomycin increases the electrical driving force for Na+ during Na+-coupled galactose entry into the enterocyte.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bieberdorf F. A., Morawski S., Fordtran J. S. Effect of sodium, mannitol, and magnesium on glucose, galactose, 3-O-methylglucose, and fructose absorption in the human ileum. Gastroenterology. 1975 Jan;68(1):58–66. [PubMed] [Google Scholar]
  2. Broitman S. A., Flint D., Zamcheck N. Neomycin feeding in the rat: studies of intestinal absorption of glucose and xylose. J Lab Clin Med. 1967 Jul;70(1):9–15. [PubMed] [Google Scholar]
  3. Carter-Su C., Kimmich G. A. Effect of membrane potential on Na+-dependent sugar transport by ATP-depleted intestinal cells. Am J Physiol. 1980 Mar;238(3):C73–C80. doi: 10.1152/ajpcell.1980.238.3.C73. [DOI] [PubMed] [Google Scholar]
  4. Cremaschi D., James P. S., Meyer G., Peacock M. A., Smith M. W. Membrane potentials of differentiating enterocytes. Biochim Biophys Acta. 1982 May 21;688(1):271–274. doi: 10.1016/0005-2736(82)90603-4. [DOI] [PubMed] [Google Scholar]
  5. Debnam E. S. Effect of sodium concentration and plasma sugar concentration on hexose absorption by the rat jejunum in vivo. Further evidence of two transport mechanisms. Pflugers Arch. 1982 Mar;393(1):104–108. doi: 10.1007/BF00582401. [DOI] [PubMed] [Google Scholar]
  6. Debnam E. S., Levin R. J. An experimental method of identifying and quantifying the active transfer electrogenic component from the diffusive component during sugar absorption measured in vivo. J Physiol. 1975 Mar;246(1):181–196. doi: 10.1113/jphysiol.1975.sp010885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Elsenhans B., Blume R., Lembcke B., Caspary W. F. A new class of inhibitors for in vitro small intestinal transport of sugars and amino acids in the rat. Biochim Biophys Acta. 1983 Jan 5;727(1):135–143. doi: 10.1016/0005-2736(83)90377-2. [DOI] [PubMed] [Google Scholar]
  8. FEINGOLD D. S. ANTIMICROBIAL CHEMOTHERAPEUTIC AGENTS: THE NATURE OF THEIR ACTION AND SELECTIVE TOXICITY. N Engl J Med. 1963 Oct 24;269:900–CONTD. doi: 10.1056/NEJM196310242691706. [DOI] [PubMed] [Google Scholar]
  9. Kessler J. I., Sehgal A. K., Turcotte R. Effect of neomycin on amino acid uptake and on synthesis and release of lipoproteins by rat intestine. Can J Physiol Pharmacol. 1978 Jun;56(3):420–427. doi: 10.1139/y78-063. [DOI] [PubMed] [Google Scholar]
  10. Lemaire J., Maestracci D., Laprade R., Sauvé R. Mechanism of neomycin stimulation of D-glucose uptake in rabbit intestinal brush border membrane. Biochim Biophys Acta. 1982 Mar 23;686(1):119–129. doi: 10.1016/0005-2736(82)90157-2. [DOI] [PubMed] [Google Scholar]
  11. Murakami E., Saito M., Suda M. Contribution of diffusive pathway in intestinal absorption of glucose in rat under normal feeding condition. Experientia. 1977 Nov 15;33(11):1469–1470. doi: 10.1007/BF01918813. [DOI] [PubMed] [Google Scholar]
  12. Robinson J. W., Antonioli J. A., Fasel J. The effect of oral neomycin on the rat intestine. Gastroenterologia. 1966;105(3):129–138. doi: 10.1159/000202042. [DOI] [PubMed] [Google Scholar]
  13. Rogers A. I., Bachorik P. S., Nunn A. S. Neomycin effects on glucose transport by rat small intestine. Digestion. 1968;1(3):159–164. doi: 10.1159/000196848. [DOI] [PubMed] [Google Scholar]
  14. Schultz S. G. Sodium-coupled solute transport of small intestine: a status report. Am J Physiol. 1977 Oct;233(4):E249–E254. doi: 10.1152/ajpendo.1977.233.4.E249. [DOI] [PubMed] [Google Scholar]
  15. Small M. D., Folen V., Abrams G., Bledsoe F., Norris H. T., Sprinz H. High doses of neomycin sulfate and the absorption of glucose and xylose from the rat small intestine. Am J Dig Dis. 1966 Dec;11(12):923–929. doi: 10.1007/BF02233080. [DOI] [PubMed] [Google Scholar]

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